Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaG and rfaP overlap by 8 nucleotides rfaP and waaO are separated by 42 nucleotides waaO and rfaY are separated by 25 nucleotides rfaY and rfaJ are separated by 24 nucleotides
JDDGAC_19280: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 3,667,078 to 3,668,202
rfaG
JDDGAC_19285: rfaP - lipopolysaccharide core heptose(I) kinase RfaP, at 3,668,195 to 3,668,992
rfaP
JDDGAC_19290: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 3,669,035 to 3,670,042
waaO
JDDGAC_19295: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 3,670,068 to 3,670,775
rfaY
JDDGAC_19300: rfaJ - glucosyltransferase, at 3,670,800 to 3,671,813
rfaJ
Position (kb)
3669
3670
3671 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3668.128 kb on + strand at 3668.136 kb on + strand at 3668.136 kb on + strand at 3668.180 kb on + strand at 3668.333 kb on + strand, within rfaP at 3668.406 kb on + strand, within rfaP at 3668.467 kb on + strand, within rfaP at 3668.467 kb on + strand, within rfaP at 3668.467 kb on + strand, within rfaP at 3668.467 kb on + strand, within rfaP at 3668.467 kb on + strand, within rfaP at 3668.554 kb on - strand, within rfaP at 3668.614 kb on + strand, within rfaP at 3668.685 kb on - strand, within rfaP at 3668.686 kb on + strand, within rfaP at 3668.873 kb on - strand, within rfaP at 3669.003 kb on - strand at 3669.120 kb on + strand at 3669.228 kb on - strand, within waaO at 3669.383 kb on + strand, within waaO at 3669.445 kb on + strand, within waaO at 3669.763 kb on + strand, within waaO at 3669.819 kb on + strand, within waaO at 3669.820 kb on - strand, within waaO at 3669.824 kb on + strand, within waaO at 3669.925 kb on - strand, within waaO at 3670.067 kb on + strand at 3670.074 kb on + strand at 3670.384 kb on + strand, within rfaY at 3670.757 kb on - strand at 3670.984 kb on + strand, within rfaJ at 3671.010 kb on + strand, within rfaJ
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 3,668,128 + -3.1 3,668,136 + -2.2 3,668,136 + +1.1 3,668,180 + -1.1 3,668,333 + rfaP JDDGAC_19285 0.17 -2.3 3,668,406 + rfaP JDDGAC_19285 0.26 -1.7 3,668,467 + rfaP JDDGAC_19285 0.34 -2.7 3,668,467 + rfaP JDDGAC_19285 0.34 -0.9 3,668,467 + rfaP JDDGAC_19285 0.34 -0.3 3,668,467 + rfaP JDDGAC_19285 0.34 -1.2 3,668,467 + rfaP JDDGAC_19285 0.34 -1.0 3,668,554 - rfaP JDDGAC_19285 0.45 -1.0 3,668,614 + rfaP JDDGAC_19285 0.53 -2.1 3,668,685 - rfaP JDDGAC_19285 0.61 -2.8 3,668,686 + rfaP JDDGAC_19285 0.62 -0.3 3,668,873 - rfaP JDDGAC_19285 0.85 -2.2 3,669,003 - -3.8 3,669,120 + -5.3 3,669,228 - waaO JDDGAC_19290 0.19 -2.9 3,669,383 + waaO JDDGAC_19290 0.35 -4.3 3,669,445 + waaO JDDGAC_19290 0.41 -4.8 3,669,763 + waaO JDDGAC_19290 0.72 -5.2 3,669,819 + waaO JDDGAC_19290 0.78 -3.5 3,669,820 - waaO JDDGAC_19290 0.78 -3.0 3,669,824 + waaO JDDGAC_19290 0.78 -3.2 3,669,925 - waaO JDDGAC_19290 0.88 -1.8 3,670,067 + -0.5 3,670,074 + -0.0 3,670,384 + rfaY JDDGAC_19295 0.45 -0.4 3,670,757 - -4.4 3,670,984 + rfaJ JDDGAC_19300 0.18 -2.7 3,671,010 + rfaJ JDDGAC_19300 0.21 -5.6
Or see this region's nucleotide sequence