Strain Fitness in Escherichia coli ECRC98 around JDDGAC_19290

Experiment: WM_TP12

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaG and rfaP overlap by 8 nucleotidesrfaP and waaO are separated by 42 nucleotideswaaO and rfaY are separated by 25 nucleotidesrfaY and rfaJ are separated by 24 nucleotides JDDGAC_19280: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 3,667,078 to 3,668,202 rfaG JDDGAC_19285: rfaP - lipopolysaccharide core heptose(I) kinase RfaP, at 3,668,195 to 3,668,992 rfaP JDDGAC_19290: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 3,669,035 to 3,670,042 waaO JDDGAC_19295: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 3,670,068 to 3,670,775 rfaY JDDGAC_19300: rfaJ - glucosyltransferase, at 3,670,800 to 3,671,813 rfaJ Position (kb) 3669 3670 3671Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3668.128 kb on + strandat 3668.136 kb on + strandat 3668.136 kb on + strandat 3668.180 kb on + strandat 3668.333 kb on + strand, within rfaPat 3668.406 kb on + strand, within rfaPat 3668.467 kb on + strand, within rfaPat 3668.467 kb on + strand, within rfaPat 3668.467 kb on + strand, within rfaPat 3668.467 kb on + strand, within rfaPat 3668.467 kb on + strand, within rfaPat 3668.554 kb on - strand, within rfaPat 3668.614 kb on + strand, within rfaPat 3668.685 kb on - strand, within rfaPat 3668.686 kb on + strand, within rfaPat 3668.873 kb on - strand, within rfaPat 3669.003 kb on - strandat 3669.120 kb on + strandat 3669.228 kb on - strand, within waaOat 3669.383 kb on + strand, within waaOat 3669.445 kb on + strand, within waaOat 3669.763 kb on + strand, within waaOat 3669.819 kb on + strand, within waaOat 3669.820 kb on - strand, within waaOat 3669.824 kb on + strand, within waaOat 3669.925 kb on - strand, within waaOat 3670.067 kb on + strandat 3670.074 kb on + strandat 3670.384 kb on + strand, within rfaYat 3670.757 kb on - strandat 3670.984 kb on + strand, within rfaJat 3671.010 kb on + strand, within rfaJ

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP12
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3,668,128 + -3.1
3,668,136 + -2.2
3,668,136 + +1.1
3,668,180 + -1.1
3,668,333 + rfaP JDDGAC_19285 0.17 -2.3
3,668,406 + rfaP JDDGAC_19285 0.26 -1.7
3,668,467 + rfaP JDDGAC_19285 0.34 -2.7
3,668,467 + rfaP JDDGAC_19285 0.34 -0.9
3,668,467 + rfaP JDDGAC_19285 0.34 -0.3
3,668,467 + rfaP JDDGAC_19285 0.34 -1.2
3,668,467 + rfaP JDDGAC_19285 0.34 -1.0
3,668,554 - rfaP JDDGAC_19285 0.45 -1.0
3,668,614 + rfaP JDDGAC_19285 0.53 -2.1
3,668,685 - rfaP JDDGAC_19285 0.61 -2.8
3,668,686 + rfaP JDDGAC_19285 0.62 -0.3
3,668,873 - rfaP JDDGAC_19285 0.85 -2.2
3,669,003 - -3.8
3,669,120 + -5.3
3,669,228 - waaO JDDGAC_19290 0.19 -2.9
3,669,383 + waaO JDDGAC_19290 0.35 -4.3
3,669,445 + waaO JDDGAC_19290 0.41 -4.8
3,669,763 + waaO JDDGAC_19290 0.72 -5.2
3,669,819 + waaO JDDGAC_19290 0.78 -3.5
3,669,820 - waaO JDDGAC_19290 0.78 -3.0
3,669,824 + waaO JDDGAC_19290 0.78 -3.2
3,669,925 - waaO JDDGAC_19290 0.88 -1.8
3,670,067 + -0.5
3,670,074 + -0.0
3,670,384 + rfaY JDDGAC_19295 0.45 -0.4
3,670,757 - -4.4
3,670,984 + rfaJ JDDGAC_19300 0.18 -2.7
3,671,010 + rfaJ JDDGAC_19300 0.21 -5.6

Or see this region's nucleotide sequence