Strain Fitness in Escherichia coli ECRC98 around JDDGAC_11485

Experiment: WM_TP12

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntppiB and lpxH are separated by 2 nucleotideslpxH and purE are separated by 117 nucleotidespurE and purK overlap by 4 nucleotides JDDGAC_11475: ppiB - peptidylprolyl isomerase B, at 2,078,357 to 2,078,851 ppiB JDDGAC_11480: lpxH - UDP-2,3-diacylglucosamine diphosphatase, at 2,078,854 to 2,079,576 lpxH JDDGAC_11485: purE - N5-carboxyaminoimidazole ribonucleotide mutase, at 2,079,694 to 2,080,203 purE JDDGAC_11490: purK - 5-(carboxyamino)imidazole ribonucleotide synthase, at 2,080,200 to 2,081,267 purK Position (kb) 2079 2080 2081Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2079.686 kb on + strandat 2079.709 kb on - strandat 2079.846 kb on - strand, within purEat 2079.892 kb on - strand, within purEat 2079.971 kb on + strand, within purEat 2080.006 kb on + strand, within purEat 2080.016 kb on + strand, within purEat 2080.116 kb on + strand, within purEat 2080.242 kb on - strandat 2080.372 kb on + strand, within purKat 2080.496 kb on - strand, within purKat 2080.496 kb on - strand, within purKat 2080.537 kb on + strand, within purKat 2080.537 kb on + strand, within purKat 2080.649 kb on + strand, within purKat 2080.649 kb on + strand, within purKat 2080.650 kb on - strand, within purKat 2080.667 kb on - strand, within purKat 2080.761 kb on - strand, within purKat 2080.932 kb on - strand, within purKat 2080.981 kb on + strand, within purKat 2080.982 kb on - strand, within purKat 2081.060 kb on + strand, within purKat 2081.138 kb on + strand, within purK

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP12
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2,079,686 + +0.1
2,079,709 - -3.2
2,079,846 - purE JDDGAC_11485 0.30 -4.1
2,079,892 - purE JDDGAC_11485 0.39 -4.3
2,079,971 + purE JDDGAC_11485 0.54 -3.4
2,080,006 + purE JDDGAC_11485 0.61 -3.9
2,080,016 + purE JDDGAC_11485 0.63 -1.7
2,080,116 + purE JDDGAC_11485 0.83 -3.4
2,080,242 - -3.3
2,080,372 + purK JDDGAC_11490 0.16 -1.6
2,080,496 - purK JDDGAC_11490 0.28 -4.4
2,080,496 - purK JDDGAC_11490 0.28 -3.3
2,080,537 + purK JDDGAC_11490 0.32 -1.2
2,080,537 + purK JDDGAC_11490 0.32 -2.4
2,080,649 + purK JDDGAC_11490 0.42 -2.9
2,080,649 + purK JDDGAC_11490 0.42 -1.6
2,080,650 - purK JDDGAC_11490 0.42 -3.9
2,080,667 - purK JDDGAC_11490 0.44 -4.6
2,080,761 - purK JDDGAC_11490 0.53 -2.8
2,080,932 - purK JDDGAC_11490 0.69 -3.1
2,080,981 + purK JDDGAC_11490 0.73 -3.1
2,080,982 - purK JDDGAC_11490 0.73 -2.1
2,081,060 + purK JDDGAC_11490 0.81 -2.9
2,081,138 + purK JDDGAC_11490 0.88 -0.6

Or see this region's nucleotide sequence