Strain Fitness in Escherichia coli ECRC98 around JDDGAC_10465

Experiment: WM_TP12

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttolB and tolA are separated by 132 nucleotidestolA and tolR are separated by 64 nucleotidestolR and tolQ are separated by 3 nucleotides JDDGAC_10460: tolB - Tol-Pal system protein TolB, at 1,861,857 to 1,863,149 tolB JDDGAC_10465: tolA - cell envelope integrity protein TolA, at 1,863,282 to 1,864,496 tolA JDDGAC_10470: tolR - colicin uptake protein TolR, at 1,864,561 to 1,864,989 tolR JDDGAC_10475: tolQ - Tol-Pal system protein TolQ, at 1,864,993 to 1,865,685 tolQ Position (kb) 1863 1864 1865Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1862.335 kb on - strand, within tolBat 1862.335 kb on - strand, within tolBat 1862.339 kb on - strand, within tolBat 1862.339 kb on - strand, within tolBat 1862.443 kb on - strand, within tolBat 1862.503 kb on + strand, within tolBat 1862.504 kb on - strand, within tolBat 1862.598 kb on + strand, within tolBat 1862.644 kb on + strand, within tolBat 1862.645 kb on - strand, within tolBat 1862.672 kb on + strand, within tolBat 1862.673 kb on - strand, within tolBat 1862.767 kb on - strand, within tolBat 1862.815 kb on + strand, within tolBat 1862.816 kb on - strand, within tolBat 1862.853 kb on + strand, within tolBat 1862.893 kb on - strand, within tolBat 1862.960 kb on + strand, within tolBat 1863.058 kb on - strandat 1863.112 kb on - strandat 1863.152 kb on + strandat 1863.153 kb on - strandat 1863.162 kb on - strandat 1863.322 kb on + strandat 1863.490 kb on + strand, within tolAat 1863.490 kb on + strand, within tolAat 1863.533 kb on + strand, within tolAat 1863.534 kb on - strand, within tolAat 1863.534 kb on - strand, within tolAat 1863.635 kb on + strand, within tolAat 1863.660 kb on - strand, within tolAat 1864.422 kb on + strandat 1864.449 kb on - strandat 1864.453 kb on - strandat 1864.562 kb on + strandat 1864.563 kb on - strandat 1864.566 kb on + strandat 1864.567 kb on - strandat 1864.567 kb on - strandat 1864.609 kb on + strand, within tolRat 1864.609 kb on + strand, within tolRat 1864.626 kb on + strand, within tolRat 1864.770 kb on - strand, within tolRat 1864.918 kb on + strand, within tolRat 1864.933 kb on + strand, within tolRat 1864.934 kb on - strand, within tolRat 1865.111 kb on + strand, within tolQat 1865.270 kb on + strand, within tolQat 1865.271 kb on - strand, within tolQat 1865.271 kb on - strand, within tolQat 1865.346 kb on + strand, within tolQ

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP12
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1,862,335 - tolB JDDGAC_10460 0.37 -2.7
1,862,335 - tolB JDDGAC_10460 0.37 -0.6
1,862,339 - tolB JDDGAC_10460 0.37 -3.1
1,862,339 - tolB JDDGAC_10460 0.37 +1.0
1,862,443 - tolB JDDGAC_10460 0.45 -2.6
1,862,503 + tolB JDDGAC_10460 0.50 -1.3
1,862,504 - tolB JDDGAC_10460 0.50 -2.3
1,862,598 + tolB JDDGAC_10460 0.57 +1.6
1,862,644 + tolB JDDGAC_10460 0.61 -0.7
1,862,645 - tolB JDDGAC_10460 0.61 -2.9
1,862,672 + tolB JDDGAC_10460 0.63 -1.7
1,862,673 - tolB JDDGAC_10460 0.63 -0.8
1,862,767 - tolB JDDGAC_10460 0.70 +0.5
1,862,815 + tolB JDDGAC_10460 0.74 -1.3
1,862,816 - tolB JDDGAC_10460 0.74 -4.2
1,862,853 + tolB JDDGAC_10460 0.77 -0.1
1,862,893 - tolB JDDGAC_10460 0.80 +0.4
1,862,960 + tolB JDDGAC_10460 0.85 +1.6
1,863,058 - -3.2
1,863,112 - -0.0
1,863,152 + -1.3
1,863,153 - -1.8
1,863,162 - -0.3
1,863,322 + -1.3
1,863,490 + tolA JDDGAC_10465 0.17 -1.6
1,863,490 + tolA JDDGAC_10465 0.17 -0.5
1,863,533 + tolA JDDGAC_10465 0.21 -1.2
1,863,534 - tolA JDDGAC_10465 0.21 -2.2
1,863,534 - tolA JDDGAC_10465 0.21 -1.5
1,863,635 + tolA JDDGAC_10465 0.29 -0.6
1,863,660 - tolA JDDGAC_10465 0.31 -1.0
1,864,422 + -1.5
1,864,449 - -1.7
1,864,453 - +1.0
1,864,562 + -0.3
1,864,563 - +0.3
1,864,566 + -0.0
1,864,567 - -0.3
1,864,567 - +0.9
1,864,609 + tolR JDDGAC_10470 0.11 -0.8
1,864,609 + tolR JDDGAC_10470 0.11 -0.3
1,864,626 + tolR JDDGAC_10470 0.15 -2.5
1,864,770 - tolR JDDGAC_10470 0.49 -0.0
1,864,918 + tolR JDDGAC_10470 0.83 -3.8
1,864,933 + tolR JDDGAC_10470 0.87 +1.0
1,864,934 - tolR JDDGAC_10470 0.87 +1.0
1,865,111 + tolQ JDDGAC_10475 0.17 -1.6
1,865,270 + tolQ JDDGAC_10475 0.40 -1.6
1,865,271 - tolQ JDDGAC_10475 0.40 -1.5
1,865,271 - tolQ JDDGAC_10475 0.40 -1.6
1,865,346 + tolQ JDDGAC_10475 0.51 -0.6

Or see this region's nucleotide sequence