Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspS and nudB are separated by 60 nucleotides nudB and JDDGAC_00830 are separated by 28 nucleotides JDDGAC_00830 and JDDGAC_00835 overlap by 1 nucleotides JDDGAC_00835 and ruvC overlap by 1 nucleotides
JDDGAC_00820: aspS - aspartate--tRNA ligase, at 136,787 to 138,559
aspS
JDDGAC_00825: nudB - dihydroneopterin triphosphate diphosphatase, at 138,620 to 139,129
nudB
JDDGAC_00830: JDDGAC_00830 - putative transcriptional regulatory protein YebC, at 139,158 to 139,898
_00830
JDDGAC_00835: JDDGAC_00835 - Crossover junction endodeoxyribonuclease RuvC, at 139,898 to 139,933
_00835
JDDGAC_00840: ruvC - crossover junction endodeoxyribonuclease RuvC, at 139,933 to 140,454
ruvC
Position (kb) 138
139
140 Strain fitness (log2 ratio) -2
-1
0
1
2 at 138.627 kb on + strand at 138.628 kb on - strand at 138.628 kb on - strand at 138.631 kb on + strand at 138.631 kb on + strand at 138.631 kb on + strand at 138.631 kb on + strand at 138.631 kb on + strand at 138.631 kb on + strand at 138.631 kb on + strand at 138.631 kb on + strand at 138.632 kb on - strand at 138.635 kb on + strand at 138.635 kb on + strand at 138.635 kb on + strand at 138.635 kb on + strand at 138.636 kb on - strand at 138.636 kb on - strand at 138.725 kb on + strand, within nudB at 138.936 kb on + strand, within nudB at 139.117 kb on + strand at 139.142 kb on - strand at 139.172 kb on + strand at 139.197 kb on - strand at 139.416 kb on + strand, within JDDGAC_00830 at 139.650 kb on + strand, within JDDGAC_00830 at 139.674 kb on + strand, within JDDGAC_00830 at 139.674 kb on + strand, within JDDGAC_00830 at 139.997 kb on + strand, within ruvC at 140.033 kb on + strand, within ruvC at 140.055 kb on - strand, within ruvC
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 138,627 + +0.1 138,628 - -1.9 138,628 - -1.5 138,631 + -1.0 138,631 + +0.1 138,631 + -2.4 138,631 + +0.7 138,631 + +0.3 138,631 + +1.4 138,631 + -2.4 138,631 + -0.9 138,632 - -2.6 138,635 + -0.1 138,635 + +0.1 138,635 + +0.0 138,635 + +0.6 138,636 - -0.8 138,636 - -2.4 138,725 + nudB JDDGAC_00825 0.21 -1.5 138,936 + nudB JDDGAC_00825 0.62 -0.5 139,117 + +2.3 139,142 - -0.8 139,172 + +0.0 139,197 - -1.2 139,416 + JDDGAC_00830 0.35 +0.2 139,650 + JDDGAC_00830 0.66 -0.5 139,674 + JDDGAC_00830 0.70 -2.3 139,674 + JDDGAC_00830 0.70 -0.6 139,997 + ruvC JDDGAC_00840 0.12 -0.0 140,033 + ruvC JDDGAC_00840 0.19 -0.1 140,055 - ruvC JDDGAC_00840 0.23 -1.8
Or see this region's nucleotide sequence