Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaD and wcaE are separated by 10 nucleotides wcaE and wcaF are separated by 15 nucleotides wcaF and JDDGAC_28455 are separated by 28 nucleotides
JDDGAC_28440: wcaD - putative colanic acid polymerase WcaD, at 5,452,983 to 5,454,200
wcaD
JDDGAC_28445: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 5,454,211 to 5,454,957
wcaE
JDDGAC_28450: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 5,454,973 to 5,455,521
wcaF
JDDGAC_28455: JDDGAC_28455 - GDP-mannose 4,6-dehydratase, at 5,455,550 to 5,456,671
_28455
Position (kb)
5454
5455
5456 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5454.390 kb on - strand, within wcaE at 5454.390 kb on - strand, within wcaE at 5454.390 kb on - strand, within wcaE at 5455.030 kb on - strand, within wcaF at 5455.085 kb on + strand, within wcaF at 5455.112 kb on + strand, within wcaF at 5455.289 kb on + strand, within wcaF at 5455.289 kb on + strand, within wcaF at 5455.544 kb on + strand at 5455.578 kb on + strand at 5455.643 kb on - strand at 5455.643 kb on - strand at 5455.645 kb on + strand at 5455.645 kb on + strand at 5455.646 kb on - strand at 5455.751 kb on - strand, within JDDGAC_28455 at 5455.768 kb on + strand, within JDDGAC_28455 at 5455.800 kb on - strand, within JDDGAC_28455 at 5455.876 kb on + strand, within JDDGAC_28455 at 5456.037 kb on - strand, within JDDGAC_28455 at 5456.072 kb on + strand, within JDDGAC_28455 at 5456.081 kb on + strand, within JDDGAC_28455 at 5456.081 kb on + strand, within JDDGAC_28455 at 5456.082 kb on - strand, within JDDGAC_28455 at 5456.082 kb on - strand, within JDDGAC_28455 at 5456.082 kb on - strand, within JDDGAC_28455 at 5456.082 kb on - strand, within JDDGAC_28455 at 5456.082 kb on - strand, within JDDGAC_28455 at 5456.095 kb on + strand, within JDDGAC_28455 at 5456.209 kb on + strand, within JDDGAC_28455 at 5456.319 kb on - strand, within JDDGAC_28455 at 5456.337 kb on + strand, within JDDGAC_28455
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 5,454,390 - wcaE JDDGAC_28445 0.24 +0.9 5,454,390 - wcaE JDDGAC_28445 0.24 -1.3 5,454,390 - wcaE JDDGAC_28445 0.24 +0.5 5,455,030 - wcaF JDDGAC_28450 0.10 +0.4 5,455,085 + wcaF JDDGAC_28450 0.20 -0.2 5,455,112 + wcaF JDDGAC_28450 0.25 +0.3 5,455,289 + wcaF JDDGAC_28450 0.58 -1.6 5,455,289 + wcaF JDDGAC_28450 0.58 -3.7 5,455,544 + +0.5 5,455,578 + -0.3 5,455,643 - -1.7 5,455,643 - +1.5 5,455,645 + +0.4 5,455,645 + +0.9 5,455,646 - -0.3 5,455,751 - JDDGAC_28455 0.18 +0.2 5,455,768 + JDDGAC_28455 0.19 -0.4 5,455,800 - JDDGAC_28455 0.22 -0.4 5,455,876 + JDDGAC_28455 0.29 +0.5 5,456,037 - JDDGAC_28455 0.43 -0.5 5,456,072 + JDDGAC_28455 0.47 +0.5 5,456,081 + JDDGAC_28455 0.47 -0.1 5,456,081 + JDDGAC_28455 0.47 +0.7 5,456,082 - JDDGAC_28455 0.47 +0.7 5,456,082 - JDDGAC_28455 0.47 +0.1 5,456,082 - JDDGAC_28455 0.47 +0.4 5,456,082 - JDDGAC_28455 0.47 -0.0 5,456,082 - JDDGAC_28455 0.47 +0.2 5,456,095 + JDDGAC_28455 0.49 +0.3 5,456,209 + JDDGAC_28455 0.59 -0.9 5,456,319 - JDDGAC_28455 0.69 -0.4 5,456,337 + JDDGAC_28455 0.70 +0.4
Or see this region's nucleotide sequence