Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bisC and yiaC overlap by 32 nucleotides yiaC and tag overlap by 4 nucleotides tag and yhjY are separated by 157 nucleotides
JDDGAC_19660: bisC - biotin sulfoxide reductase, at 3,754,040 to 3,756,373
bisC
JDDGAC_19665: yiaC - N-acetyltransferase, at 3,756,342 to 3,756,782
yiaC
JDDGAC_19670: tag - DNA-3-methyladenine glycosylase I, at 3,756,779 to 3,757,342
tag
JDDGAC_19675: yhjY - Uncharacterized protein YhjY, at 3,757,500 to 3,758,198
yhjY
Position (kb)
3756
3757
3758 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3755.833 kb on + strand, within bisC at 3755.858 kb on - strand, within bisC at 3755.867 kb on + strand, within bisC at 3755.868 kb on - strand, within bisC at 3755.946 kb on + strand, within bisC at 3755.947 kb on - strand, within bisC at 3755.952 kb on + strand, within bisC at 3755.997 kb on - strand, within bisC at 3755.997 kb on - strand, within bisC at 3756.017 kb on + strand, within bisC at 3756.017 kb on + strand, within bisC at 3756.078 kb on + strand, within bisC at 3756.078 kb on + strand, within bisC at 3756.154 kb on + strand at 3756.154 kb on + strand at 3756.154 kb on + strand at 3756.154 kb on + strand at 3756.155 kb on - strand at 3756.155 kb on - strand at 3756.217 kb on + strand at 3756.217 kb on + strand at 3756.217 kb on + strand at 3756.218 kb on - strand at 3756.295 kb on + strand at 3756.296 kb on - strand at 3756.320 kb on + strand at 3756.343 kb on + strand at 3756.344 kb on - strand at 3756.384 kb on + strand at 3756.384 kb on + strand at 3756.384 kb on + strand at 3756.384 kb on + strand at 3756.385 kb on - strand at 3756.509 kb on - strand, within yiaC at 3756.509 kb on - strand, within yiaC at 3756.509 kb on - strand, within yiaC at 3756.509 kb on - strand, within yiaC at 3756.509 kb on - strand, within yiaC at 3756.509 kb on - strand, within yiaC at 3756.509 kb on - strand, within yiaC at 3756.588 kb on - strand, within yiaC at 3756.652 kb on + strand, within yiaC at 3756.652 kb on + strand, within yiaC at 3756.653 kb on - strand, within yiaC at 3756.722 kb on + strand, within yiaC at 3756.722 kb on + strand, within yiaC at 3756.723 kb on - strand, within yiaC at 3756.723 kb on - strand, within yiaC at 3756.788 kb on - strand at 3756.847 kb on + strand, within tag at 3756.928 kb on + strand, within tag at 3756.928 kb on + strand, within tag at 3757.038 kb on + strand, within tag at 3757.058 kb on - strand, within tag at 3757.288 kb on + strand at 3757.288 kb on + strand at 3757.303 kb on + strand at 3757.304 kb on - strand at 3757.304 kb on - strand at 3757.306 kb on - strand at 3757.306 kb on - strand at 3757.342 kb on + strand at 3757.355 kb on + strand at 3757.355 kb on + strand at 3757.355 kb on + strand at 3757.356 kb on - strand at 3757.356 kb on - strand at 3757.356 kb on - strand at 3757.356 kb on - strand at 3757.356 kb on - strand at 3757.360 kb on - strand at 3757.418 kb on + strand at 3757.418 kb on + strand at 3757.419 kb on - strand at 3757.429 kb on + strand at 3757.429 kb on + strand at 3757.429 kb on + strand at 3757.430 kb on - strand at 3757.432 kb on + strand at 3757.450 kb on + strand at 3757.450 kb on + strand at 3757.451 kb on - strand at 3757.461 kb on + strand at 3757.461 kb on + strand at 3757.462 kb on - strand at 3757.462 kb on - strand at 3757.462 kb on - strand at 3757.499 kb on - strand at 3757.534 kb on + strand at 3757.535 kb on - strand at 3757.535 kb on - strand at 3757.602 kb on + strand, within yhjY at 3757.602 kb on + strand, within yhjY at 3757.602 kb on + strand, within yhjY at 3757.603 kb on - strand, within yhjY at 3757.603 kb on - strand, within yhjY at 3757.603 kb on - strand, within yhjY at 3757.603 kb on - strand, within yhjY at 3757.604 kb on + strand, within yhjY at 3757.604 kb on + strand, within yhjY at 3757.604 kb on + strand, within yhjY at 3757.614 kb on - strand, within yhjY at 3757.641 kb on + strand, within yhjY at 3757.653 kb on - strand, within yhjY at 3757.695 kb on - strand, within yhjY at 3757.795 kb on + strand, within yhjY at 3757.798 kb on - strand, within yhjY at 3757.808 kb on + strand, within yhjY at 3757.832 kb on - strand, within yhjY at 3757.837 kb on - strand, within yhjY at 3757.860 kb on + strand, within yhjY at 3757.860 kb on + strand, within yhjY at 3757.860 kb on + strand, within yhjY at 3757.861 kb on - strand, within yhjY at 3757.861 kb on - strand, within yhjY at 3757.880 kb on + strand, within yhjY at 3757.881 kb on - strand, within yhjY at 3757.983 kb on + strand, within yhjY at 3757.983 kb on + strand, within yhjY at 3757.984 kb on - strand, within yhjY at 3757.985 kb on + strand, within yhjY at 3757.986 kb on - strand, within yhjY at 3758.090 kb on + strand, within yhjY at 3758.090 kb on + strand, within yhjY at 3758.091 kb on - strand, within yhjY at 3758.091 kb on - strand, within yhjY at 3758.129 kb on - strand at 3758.144 kb on + strand at 3758.150 kb on + strand at 3758.163 kb on + strand at 3758.170 kb on - strand at 3758.171 kb on + strand at 3758.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 3,755,833 + bisC JDDGAC_19660 0.77 -1.2 3,755,858 - bisC JDDGAC_19660 0.78 -0.3 3,755,867 + bisC JDDGAC_19660 0.78 -0.2 3,755,868 - bisC JDDGAC_19660 0.78 -0.1 3,755,946 + bisC JDDGAC_19660 0.82 +0.9 3,755,947 - bisC JDDGAC_19660 0.82 -0.6 3,755,952 + bisC JDDGAC_19660 0.82 -0.1 3,755,997 - bisC JDDGAC_19660 0.84 +0.0 3,755,997 - bisC JDDGAC_19660 0.84 -0.2 3,756,017 + bisC JDDGAC_19660 0.85 -0.2 3,756,017 + bisC JDDGAC_19660 0.85 +0.3 3,756,078 + bisC JDDGAC_19660 0.87 +2.5 3,756,078 + bisC JDDGAC_19660 0.87 -1.8 3,756,154 + +0.4 3,756,154 + -0.3 3,756,154 + +0.3 3,756,154 + +0.9 3,756,155 - -0.9 3,756,155 - -0.2 3,756,217 + +1.2 3,756,217 + +0.4 3,756,217 + +1.2 3,756,218 - -0.9 3,756,295 + -0.5 3,756,296 - -0.2 3,756,320 + -0.0 3,756,343 + +2.0 3,756,344 - -0.8 3,756,384 + -1.1 3,756,384 + -1.0 3,756,384 + -0.2 3,756,384 + +0.2 3,756,385 - -0.7 3,756,509 - yiaC JDDGAC_19665 0.38 -0.8 3,756,509 - yiaC JDDGAC_19665 0.38 +0.2 3,756,509 - yiaC JDDGAC_19665 0.38 +0.2 3,756,509 - yiaC JDDGAC_19665 0.38 -1.2 3,756,509 - yiaC JDDGAC_19665 0.38 -0.4 3,756,509 - yiaC JDDGAC_19665 0.38 +0.1 3,756,509 - yiaC JDDGAC_19665 0.38 -0.5 3,756,588 - yiaC JDDGAC_19665 0.56 +0.2 3,756,652 + yiaC JDDGAC_19665 0.70 -2.2 3,756,652 + yiaC JDDGAC_19665 0.70 -0.8 3,756,653 - yiaC JDDGAC_19665 0.71 +1.0 3,756,722 + yiaC JDDGAC_19665 0.86 -0.5 3,756,722 + yiaC JDDGAC_19665 0.86 +0.3 3,756,723 - yiaC JDDGAC_19665 0.86 -0.4 3,756,723 - yiaC JDDGAC_19665 0.86 +0.0 3,756,788 - +0.6 3,756,847 + tag JDDGAC_19670 0.12 -0.3 3,756,928 + tag JDDGAC_19670 0.26 -0.3 3,756,928 + tag JDDGAC_19670 0.26 -0.7 3,757,038 + tag JDDGAC_19670 0.46 -0.0 3,757,058 - tag JDDGAC_19670 0.49 -1.5 3,757,288 + +0.3 3,757,288 + +0.5 3,757,303 + +0.6 3,757,304 - +0.3 3,757,304 - +0.1 3,757,306 - +0.4 3,757,306 - +0.2 3,757,342 + -0.7 3,757,355 + -0.8 3,757,355 + +0.4 3,757,355 + -1.1 3,757,356 - +0.6 3,757,356 - +0.1 3,757,356 - -0.9 3,757,356 - -0.3 3,757,356 - -3.9 3,757,360 - -1.5 3,757,418 + +0.1 3,757,418 + -0.1 3,757,419 - -0.0 3,757,429 + -2.5 3,757,429 + +0.5 3,757,429 + -0.3 3,757,430 - -1.5 3,757,432 + +1.0 3,757,450 + +0.4 3,757,450 + -0.4 3,757,451 - -0.2 3,757,461 + +0.6 3,757,461 + -0.3 3,757,462 - +0.3 3,757,462 - +0.4 3,757,462 - +0.4 3,757,499 - -1.9 3,757,534 + -0.6 3,757,535 - -2.3 3,757,535 - +0.2 3,757,602 + yhjY JDDGAC_19675 0.15 -1.7 3,757,602 + yhjY JDDGAC_19675 0.15 +0.1 3,757,602 + yhjY JDDGAC_19675 0.15 +1.3 3,757,603 - yhjY JDDGAC_19675 0.15 +0.6 3,757,603 - yhjY JDDGAC_19675 0.15 +0.1 3,757,603 - yhjY JDDGAC_19675 0.15 -0.2 3,757,603 - yhjY JDDGAC_19675 0.15 +0.3 3,757,604 + yhjY JDDGAC_19675 0.15 -0.3 3,757,604 + yhjY JDDGAC_19675 0.15 +1.0 3,757,604 + yhjY JDDGAC_19675 0.15 -0.5 3,757,614 - yhjY JDDGAC_19675 0.16 -0.9 3,757,641 + yhjY JDDGAC_19675 0.20 +2.1 3,757,653 - yhjY JDDGAC_19675 0.22 -0.8 3,757,695 - yhjY JDDGAC_19675 0.28 +0.5 3,757,795 + yhjY JDDGAC_19675 0.42 -0.1 3,757,798 - yhjY JDDGAC_19675 0.43 +1.7 3,757,808 + yhjY JDDGAC_19675 0.44 -0.4 3,757,832 - yhjY JDDGAC_19675 0.47 +0.2 3,757,837 - yhjY JDDGAC_19675 0.48 -0.6 3,757,860 + yhjY JDDGAC_19675 0.52 -0.5 3,757,860 + yhjY JDDGAC_19675 0.52 -0.4 3,757,860 + yhjY JDDGAC_19675 0.52 -1.1 3,757,861 - yhjY JDDGAC_19675 0.52 -0.2 3,757,861 - yhjY JDDGAC_19675 0.52 -1.3 3,757,880 + yhjY JDDGAC_19675 0.54 -2.6 3,757,881 - yhjY JDDGAC_19675 0.55 -0.5 3,757,983 + yhjY JDDGAC_19675 0.69 +0.4 3,757,983 + yhjY JDDGAC_19675 0.69 -1.4 3,757,984 - yhjY JDDGAC_19675 0.69 +0.2 3,757,985 + yhjY JDDGAC_19675 0.69 +0.5 3,757,986 - yhjY JDDGAC_19675 0.70 -0.6 3,758,090 + yhjY JDDGAC_19675 0.84 -0.3 3,758,090 + yhjY JDDGAC_19675 0.84 -0.1 3,758,091 - yhjY JDDGAC_19675 0.85 -1.2 3,758,091 - yhjY JDDGAC_19675 0.85 +0.3 3,758,129 - -0.1 3,758,144 + +3.2 3,758,150 + -0.1 3,758,163 + -2.1 3,758,170 - +0.0 3,758,171 + -0.7 3,758,172 - -2.2
Or see this region's nucleotide sequence