Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiE and thiF overlap by 8 nucleotides thiF and thiS overlap by 17 nucleotides thiS and thiG are separated by 1 nucleotides thiG and thiH overlap by 4 nucleotides
JDDGAC_17005: thiE - thiamine phosphate synthase, at 3,214,947 to 3,215,582
thiE
JDDGAC_17010: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 3,215,575 to 3,216,330
thiF
JDDGAC_17015: thiS - sulfur carrier protein ThiS, at 3,216,314 to 3,216,514
thiS
JDDGAC_17020: thiG - thiazole synthase, at 3,216,516 to 3,217,286
thiG
JDDGAC_17025: thiH - 2-iminoacetate synthase ThiH, at 3,217,283 to 3,218,416
thiH
Position (kb)
3216
3217
3218 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3215.601 kb on + strand at 3215.709 kb on - strand, within thiF at 3215.808 kb on + strand, within thiF at 3215.882 kb on + strand, within thiF at 3215.882 kb on + strand, within thiF at 3215.882 kb on + strand, within thiF at 3215.883 kb on - strand, within thiF at 3215.883 kb on - strand, within thiF at 3215.883 kb on - strand, within thiF at 3215.909 kb on + strand, within thiF at 3215.909 kb on + strand, within thiF at 3215.959 kb on - strand, within thiF at 3215.979 kb on + strand, within thiF at 3215.980 kb on - strand, within thiF at 3216.013 kb on - strand, within thiF at 3216.054 kb on - strand, within thiF at 3216.082 kb on + strand, within thiF at 3216.192 kb on + strand, within thiF at 3216.200 kb on + strand, within thiF at 3216.376 kb on - strand, within thiS at 3216.460 kb on + strand, within thiS at 3216.460 kb on + strand, within thiS at 3216.461 kb on - strand, within thiS at 3216.461 kb on - strand, within thiS at 3216.461 kb on - strand, within thiS at 3216.487 kb on - strand, within thiS at 3216.521 kb on - strand at 3216.521 kb on - strand at 3216.524 kb on + strand at 3216.524 kb on + strand at 3216.524 kb on + strand at 3216.524 kb on + strand at 3216.719 kb on + strand, within thiG at 3216.719 kb on + strand, within thiG at 3216.719 kb on + strand, within thiG at 3216.720 kb on - strand, within thiG at 3216.797 kb on - strand, within thiG at 3216.874 kb on + strand, within thiG at 3217.339 kb on + strand at 3217.377 kb on + strand at 3217.405 kb on + strand, within thiH at 3217.501 kb on + strand, within thiH at 3217.509 kb on - strand, within thiH at 3217.509 kb on - strand, within thiH at 3217.509 kb on - strand, within thiH at 3217.746 kb on + strand, within thiH at 3217.759 kb on - strand, within thiH at 3217.781 kb on + strand, within thiH at 3217.781 kb on + strand, within thiH at 3217.792 kb on - strand, within thiH at 3217.835 kb on + strand, within thiH at 3217.836 kb on - strand, within thiH at 3217.836 kb on - strand, within thiH at 3217.836 kb on - strand, within thiH at 3217.850 kb on + strand, within thiH at 3217.942 kb on - strand, within thiH at 3217.991 kb on + strand, within thiH at 3217.991 kb on + strand, within thiH at 3217.991 kb on + strand, within thiH at 3217.991 kb on + strand, within thiH at 3217.991 kb on + strand, within thiH at 3217.991 kb on + strand, within thiH at 3217.992 kb on - strand, within thiH at 3217.992 kb on - strand, within thiH at 3217.992 kb on - strand, within thiH at 3217.992 kb on - strand, within thiH at 3217.992 kb on - strand, within thiH at 3217.992 kb on - strand, within thiH at 3217.993 kb on + strand, within thiH at 3217.993 kb on + strand, within thiH at 3217.994 kb on - strand, within thiH at 3217.994 kb on - strand, within thiH at 3218.077 kb on + strand, within thiH at 3218.077 kb on + strand, within thiH at 3218.078 kb on - strand, within thiH at 3218.121 kb on + strand, within thiH at 3218.122 kb on - strand, within thiH at 3218.212 kb on + strand, within thiH at 3218.227 kb on + strand, within thiH at 3218.227 kb on + strand, within thiH at 3218.228 kb on - strand, within thiH
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 3,215,601 + -0.3 3,215,709 - thiF JDDGAC_17010 0.18 +0.7 3,215,808 + thiF JDDGAC_17010 0.31 -0.4 3,215,882 + thiF JDDGAC_17010 0.41 +1.0 3,215,882 + thiF JDDGAC_17010 0.41 +2.4 3,215,882 + thiF JDDGAC_17010 0.41 +1.3 3,215,883 - thiF JDDGAC_17010 0.41 -1.6 3,215,883 - thiF JDDGAC_17010 0.41 -0.8 3,215,883 - thiF JDDGAC_17010 0.41 +0.1 3,215,909 + thiF JDDGAC_17010 0.44 +1.5 3,215,909 + thiF JDDGAC_17010 0.44 +0.8 3,215,959 - thiF JDDGAC_17010 0.51 +0.7 3,215,979 + thiF JDDGAC_17010 0.53 +1.2 3,215,980 - thiF JDDGAC_17010 0.54 +0.7 3,216,013 - thiF JDDGAC_17010 0.58 -1.2 3,216,054 - thiF JDDGAC_17010 0.63 -0.4 3,216,082 + thiF JDDGAC_17010 0.67 +1.2 3,216,192 + thiF JDDGAC_17010 0.82 +0.8 3,216,200 + thiF JDDGAC_17010 0.83 +0.2 3,216,376 - thiS JDDGAC_17015 0.31 +0.3 3,216,460 + thiS JDDGAC_17015 0.73 -0.7 3,216,460 + thiS JDDGAC_17015 0.73 -0.3 3,216,461 - thiS JDDGAC_17015 0.73 -1.9 3,216,461 - thiS JDDGAC_17015 0.73 +0.1 3,216,461 - thiS JDDGAC_17015 0.73 +1.5 3,216,487 - thiS JDDGAC_17015 0.86 +0.3 3,216,521 - -0.5 3,216,521 - +0.6 3,216,524 + +0.2 3,216,524 + +0.7 3,216,524 + +1.1 3,216,524 + +0.5 3,216,719 + thiG JDDGAC_17020 0.26 -0.3 3,216,719 + thiG JDDGAC_17020 0.26 -0.2 3,216,719 + thiG JDDGAC_17020 0.26 -0.9 3,216,720 - thiG JDDGAC_17020 0.26 +0.8 3,216,797 - thiG JDDGAC_17020 0.36 -0.4 3,216,874 + thiG JDDGAC_17020 0.46 -1.1 3,217,339 + -0.1 3,217,377 + -0.6 3,217,405 + thiH JDDGAC_17025 0.11 -0.3 3,217,501 + thiH JDDGAC_17025 0.19 -0.9 3,217,509 - thiH JDDGAC_17025 0.20 -0.8 3,217,509 - thiH JDDGAC_17025 0.20 -0.2 3,217,509 - thiH JDDGAC_17025 0.20 -0.4 3,217,746 + thiH JDDGAC_17025 0.41 -0.7 3,217,759 - thiH JDDGAC_17025 0.42 +0.0 3,217,781 + thiH JDDGAC_17025 0.44 -1.6 3,217,781 + thiH JDDGAC_17025 0.44 -0.7 3,217,792 - thiH JDDGAC_17025 0.45 +0.2 3,217,835 + thiH JDDGAC_17025 0.49 -0.4 3,217,836 - thiH JDDGAC_17025 0.49 -0.7 3,217,836 - thiH JDDGAC_17025 0.49 +0.3 3,217,836 - thiH JDDGAC_17025 0.49 -0.1 3,217,850 + thiH JDDGAC_17025 0.50 -0.9 3,217,942 - thiH JDDGAC_17025 0.58 -0.5 3,217,991 + thiH JDDGAC_17025 0.62 +1.0 3,217,991 + thiH JDDGAC_17025 0.62 +0.3 3,217,991 + thiH JDDGAC_17025 0.62 -0.2 3,217,991 + thiH JDDGAC_17025 0.62 -1.0 3,217,991 + thiH JDDGAC_17025 0.62 +0.8 3,217,991 + thiH JDDGAC_17025 0.62 +0.8 3,217,992 - thiH JDDGAC_17025 0.63 -0.6 3,217,992 - thiH JDDGAC_17025 0.63 -1.3 3,217,992 - thiH JDDGAC_17025 0.63 -0.6 3,217,992 - thiH JDDGAC_17025 0.63 -1.3 3,217,992 - thiH JDDGAC_17025 0.63 +0.5 3,217,992 - thiH JDDGAC_17025 0.63 -0.5 3,217,993 + thiH JDDGAC_17025 0.63 -0.8 3,217,993 + thiH JDDGAC_17025 0.63 +1.2 3,217,994 - thiH JDDGAC_17025 0.63 -0.2 3,217,994 - thiH JDDGAC_17025 0.63 -1.2 3,218,077 + thiH JDDGAC_17025 0.70 -0.1 3,218,077 + thiH JDDGAC_17025 0.70 -0.1 3,218,078 - thiH JDDGAC_17025 0.70 -0.7 3,218,121 + thiH JDDGAC_17025 0.74 +0.5 3,218,122 - thiH JDDGAC_17025 0.74 -0.1 3,218,212 + thiH JDDGAC_17025 0.82 +0.5 3,218,227 + thiH JDDGAC_17025 0.83 -0.3 3,218,227 + thiH JDDGAC_17025 0.83 -0.1 3,218,228 - thiH JDDGAC_17025 0.83 +0.1
Or see this region's nucleotide sequence