Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proS and yaeF are separated by 52 nucleotides yaeF and nlpE are separated by 199 nucleotides nlpE and arfB are separated by 13 nucleotides
JDDGAC_13670: proS - proline--tRNA ligase, at 2,523,877 to 2,525,595
proS
JDDGAC_13675: yaeF - YaeF family permuted papain-like enzyme, at 2,525,648 to 2,526,472
yaeF
JDDGAC_13685: nlpE - envelope stress response activation lipoprotein NlpE, at 2,526,672 to 2,527,382
nlpE
JDDGAC_13690: arfB - aminoacyl-tRNA hydrolase, at 2,527,396 to 2,527,806
arfB
Position (kb)
2525
2526
2527 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2525.631 kb on + strand at 2525.667 kb on - strand at 2525.698 kb on - strand at 2525.711 kb on - strand at 2525.752 kb on - strand, within yaeF at 2525.752 kb on - strand, within yaeF at 2525.818 kb on - strand, within yaeF at 2525.881 kb on - strand, within yaeF at 2526.013 kb on + strand, within yaeF at 2526.014 kb on - strand, within yaeF at 2526.014 kb on - strand, within yaeF at 2526.033 kb on + strand, within yaeF at 2526.100 kb on + strand, within yaeF at 2526.109 kb on + strand, within yaeF at 2526.109 kb on + strand, within yaeF at 2526.109 kb on + strand, within yaeF at 2526.110 kb on - strand, within yaeF at 2526.110 kb on - strand, within yaeF at 2526.110 kb on - strand, within yaeF at 2526.110 kb on - strand, within yaeF at 2526.113 kb on + strand, within yaeF at 2526.113 kb on + strand, within yaeF at 2526.142 kb on + strand, within yaeF at 2526.314 kb on - strand, within yaeF at 2526.359 kb on - strand, within yaeF at 2526.359 kb on - strand, within yaeF at 2526.359 kb on - strand, within yaeF at 2526.359 kb on - strand, within yaeF at 2526.364 kb on + strand, within yaeF at 2526.402 kb on + strand at 2526.407 kb on + strand at 2526.407 kb on + strand at 2526.407 kb on + strand at 2526.407 kb on + strand at 2526.408 kb on - strand at 2526.408 kb on - strand at 2526.408 kb on - strand at 2526.408 kb on - strand at 2526.434 kb on + strand at 2526.435 kb on - strand at 2526.436 kb on + strand at 2526.479 kb on - strand at 2526.479 kb on - strand at 2526.496 kb on + strand at 2526.497 kb on - strand at 2526.524 kb on + strand at 2526.524 kb on + strand at 2526.686 kb on + strand at 2526.686 kb on + strand at 2526.686 kb on + strand at 2526.687 kb on - strand at 2526.735 kb on + strand at 2526.735 kb on + strand at 2526.736 kb on - strand at 2526.736 kb on - strand at 2526.789 kb on + strand, within nlpE at 2526.789 kb on + strand, within nlpE at 2526.789 kb on + strand, within nlpE at 2526.790 kb on - strand, within nlpE at 2526.798 kb on + strand, within nlpE at 2526.799 kb on - strand, within nlpE at 2526.799 kb on - strand, within nlpE at 2526.857 kb on + strand, within nlpE at 2526.918 kb on - strand, within nlpE at 2526.925 kb on + strand, within nlpE at 2526.958 kb on - strand, within nlpE at 2526.983 kb on + strand, within nlpE at 2526.985 kb on + strand, within nlpE at 2526.986 kb on - strand, within nlpE at 2526.986 kb on - strand, within nlpE at 2526.986 kb on - strand, within nlpE at 2527.058 kb on - strand, within nlpE at 2527.086 kb on + strand, within nlpE at 2527.086 kb on + strand, within nlpE at 2527.116 kb on - strand, within nlpE at 2527.116 kb on - strand, within nlpE at 2527.160 kb on - strand, within nlpE at 2527.320 kb on - strand at 2527.334 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 2,525,631 + +0.2 2,525,667 - -0.8 2,525,698 - +1.0 2,525,711 - -0.1 2,525,752 - yaeF JDDGAC_13675 0.13 -0.0 2,525,752 - yaeF JDDGAC_13675 0.13 +1.4 2,525,818 - yaeF JDDGAC_13675 0.21 -1.1 2,525,881 - yaeF JDDGAC_13675 0.28 +1.7 2,526,013 + yaeF JDDGAC_13675 0.44 +0.3 2,526,014 - yaeF JDDGAC_13675 0.44 -1.2 2,526,014 - yaeF JDDGAC_13675 0.44 +0.6 2,526,033 + yaeF JDDGAC_13675 0.47 -0.3 2,526,100 + yaeF JDDGAC_13675 0.55 -0.2 2,526,109 + yaeF JDDGAC_13675 0.56 +0.9 2,526,109 + yaeF JDDGAC_13675 0.56 -0.5 2,526,109 + yaeF JDDGAC_13675 0.56 -1.6 2,526,110 - yaeF JDDGAC_13675 0.56 +0.4 2,526,110 - yaeF JDDGAC_13675 0.56 +0.4 2,526,110 - yaeF JDDGAC_13675 0.56 -0.2 2,526,110 - yaeF JDDGAC_13675 0.56 +0.5 2,526,113 + yaeF JDDGAC_13675 0.56 -0.2 2,526,113 + yaeF JDDGAC_13675 0.56 +0.4 2,526,142 + yaeF JDDGAC_13675 0.60 +0.1 2,526,314 - yaeF JDDGAC_13675 0.81 -0.7 2,526,359 - yaeF JDDGAC_13675 0.86 -0.1 2,526,359 - yaeF JDDGAC_13675 0.86 +0.1 2,526,359 - yaeF JDDGAC_13675 0.86 +0.5 2,526,359 - yaeF JDDGAC_13675 0.86 -0.8 2,526,364 + yaeF JDDGAC_13675 0.87 -0.1 2,526,402 + +0.4 2,526,407 + +0.2 2,526,407 + -0.2 2,526,407 + -0.2 2,526,407 + -0.6 2,526,408 - -0.6 2,526,408 - +0.1 2,526,408 - +0.5 2,526,408 - -0.5 2,526,434 + +0.7 2,526,435 - -0.4 2,526,436 + +0.0 2,526,479 - -0.1 2,526,479 - -0.8 2,526,496 + +2.7 2,526,497 - +1.0 2,526,524 + +0.2 2,526,524 + -0.2 2,526,686 + -1.4 2,526,686 + -0.6 2,526,686 + +2.4 2,526,687 - -1.8 2,526,735 + +1.2 2,526,735 + +1.5 2,526,736 - -1.0 2,526,736 - +0.0 2,526,789 + nlpE JDDGAC_13685 0.16 -0.4 2,526,789 + nlpE JDDGAC_13685 0.16 +0.4 2,526,789 + nlpE JDDGAC_13685 0.16 +1.4 2,526,790 - nlpE JDDGAC_13685 0.17 +0.2 2,526,798 + nlpE JDDGAC_13685 0.18 -0.6 2,526,799 - nlpE JDDGAC_13685 0.18 +0.0 2,526,799 - nlpE JDDGAC_13685 0.18 +1.6 2,526,857 + nlpE JDDGAC_13685 0.26 +0.3 2,526,918 - nlpE JDDGAC_13685 0.35 -0.7 2,526,925 + nlpE JDDGAC_13685 0.36 +0.2 2,526,958 - nlpE JDDGAC_13685 0.40 -0.8 2,526,983 + nlpE JDDGAC_13685 0.44 +0.2 2,526,985 + nlpE JDDGAC_13685 0.44 -0.1 2,526,986 - nlpE JDDGAC_13685 0.44 -0.6 2,526,986 - nlpE JDDGAC_13685 0.44 -0.5 2,526,986 - nlpE JDDGAC_13685 0.44 +0.3 2,527,058 - nlpE JDDGAC_13685 0.54 -0.2 2,527,086 + nlpE JDDGAC_13685 0.58 +0.8 2,527,086 + nlpE JDDGAC_13685 0.58 +0.2 2,527,116 - nlpE JDDGAC_13685 0.62 +0.0 2,527,116 - nlpE JDDGAC_13685 0.62 +1.2 2,527,160 - nlpE JDDGAC_13685 0.69 +0.4 2,527,320 - -0.3 2,527,334 + -0.8
Or see this region's nucleotide sequence