Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaQ and rnhA are separated by 55 nucleotides rnhA and yafS overlap by 4 nucleotides yafS and gloB are separated by 33 nucleotides gloB and mltD are separated by 209 nucleotides
JDDGAC_13560: dnaQ - DNA polymerase III subunit epsilon, at 2,505,633 to 2,506,373
dnaQ
JDDGAC_13565: rnhA - ribonuclease HI, at 2,506,429 to 2,506,896
rnhA
JDDGAC_13570: yafS - Uncharacterized protein YafS, at 2,506,893 to 2,507,615
yafS
JDDGAC_13575: gloB - hydroxyacylglutathione hydrolase, at 2,507,649 to 2,508,404
gloB
JDDGAC_13580: mltD - murein transglycosylase D, at 2,508,614 to 2,509,834
mltD
Position (kb)
2506
2507
2508 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2506.365 kb on - strand at 2506.376 kb on - strand at 2506.395 kb on - strand at 2506.411 kb on - strand at 2506.435 kb on - strand at 2506.505 kb on + strand, within rnhA at 2506.506 kb on - strand, within rnhA at 2506.506 kb on - strand, within rnhA at 2506.544 kb on - strand, within rnhA at 2506.646 kb on - strand, within rnhA at 2506.660 kb on - strand, within rnhA at 2506.678 kb on - strand, within rnhA at 2506.894 kb on + strand at 2506.895 kb on - strand at 2506.895 kb on - strand at 2506.958 kb on + strand at 2507.012 kb on + strand, within yafS at 2507.012 kb on + strand, within yafS at 2507.012 kb on + strand, within yafS at 2507.013 kb on - strand, within yafS at 2507.013 kb on - strand, within yafS at 2507.020 kb on - strand, within yafS at 2507.103 kb on + strand, within yafS at 2507.154 kb on - strand, within yafS at 2507.168 kb on + strand, within yafS at 2507.171 kb on - strand, within yafS at 2507.222 kb on + strand, within yafS at 2507.289 kb on + strand, within yafS at 2507.321 kb on + strand, within yafS at 2507.321 kb on + strand, within yafS at 2507.321 kb on + strand, within yafS at 2507.321 kb on + strand, within yafS at 2507.321 kb on + strand, within yafS at 2507.321 kb on + strand, within yafS at 2507.321 kb on + strand, within yafS at 2507.321 kb on + strand, within yafS at 2507.321 kb on + strand, within yafS at 2507.322 kb on - strand, within yafS at 2507.322 kb on - strand, within yafS at 2507.322 kb on - strand, within yafS at 2507.322 kb on - strand, within yafS at 2507.322 kb on - strand, within yafS at 2507.322 kb on - strand, within yafS at 2507.477 kb on + strand, within yafS at 2507.478 kb on - strand, within yafS at 2507.649 kb on - strand at 2507.657 kb on + strand at 2507.664 kb on - strand at 2507.664 kb on - strand at 2507.685 kb on + strand at 2507.752 kb on + strand, within gloB at 2507.774 kb on + strand, within gloB at 2507.871 kb on + strand, within gloB at 2507.871 kb on + strand, within gloB at 2507.872 kb on - strand, within gloB at 2507.872 kb on - strand, within gloB at 2507.894 kb on + strand, within gloB at 2507.960 kb on - strand, within gloB at 2507.960 kb on - strand, within gloB at 2507.983 kb on + strand, within gloB at 2507.990 kb on + strand, within gloB at 2508.016 kb on + strand, within gloB at 2508.123 kb on - strand, within gloB at 2508.131 kb on - strand, within gloB at 2508.149 kb on + strand, within gloB at 2508.149 kb on + strand, within gloB at 2508.149 kb on + strand, within gloB at 2508.149 kb on + strand, within gloB at 2508.150 kb on - strand, within gloB at 2508.263 kb on - strand, within gloB at 2508.263 kb on - strand, within gloB at 2508.395 kb on + strand at 2508.495 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 2,506,365 - -0.0 2,506,376 - -0.1 2,506,395 - +0.8 2,506,411 - +1.0 2,506,435 - -2.9 2,506,505 + rnhA JDDGAC_13565 0.16 +0.1 2,506,506 - rnhA JDDGAC_13565 0.16 +1.0 2,506,506 - rnhA JDDGAC_13565 0.16 +0.4 2,506,544 - rnhA JDDGAC_13565 0.25 +0.5 2,506,646 - rnhA JDDGAC_13565 0.46 -0.8 2,506,660 - rnhA JDDGAC_13565 0.49 -0.1 2,506,678 - rnhA JDDGAC_13565 0.53 -0.2 2,506,894 + -0.9 2,506,895 - -0.1 2,506,895 - +0.6 2,506,958 + +0.5 2,507,012 + yafS JDDGAC_13570 0.16 +0.6 2,507,012 + yafS JDDGAC_13570 0.16 -0.6 2,507,012 + yafS JDDGAC_13570 0.16 +0.3 2,507,013 - yafS JDDGAC_13570 0.17 +0.9 2,507,013 - yafS JDDGAC_13570 0.17 +0.1 2,507,020 - yafS JDDGAC_13570 0.18 +1.2 2,507,103 + yafS JDDGAC_13570 0.29 -0.3 2,507,154 - yafS JDDGAC_13570 0.36 +0.6 2,507,168 + yafS JDDGAC_13570 0.38 +1.6 2,507,171 - yafS JDDGAC_13570 0.38 +1.5 2,507,222 + yafS JDDGAC_13570 0.46 -1.0 2,507,289 + yafS JDDGAC_13570 0.55 +0.0 2,507,321 + yafS JDDGAC_13570 0.59 +0.2 2,507,321 + yafS JDDGAC_13570 0.59 +0.5 2,507,321 + yafS JDDGAC_13570 0.59 +0.1 2,507,321 + yafS JDDGAC_13570 0.59 -0.1 2,507,321 + yafS JDDGAC_13570 0.59 +1.1 2,507,321 + yafS JDDGAC_13570 0.59 -1.3 2,507,321 + yafS JDDGAC_13570 0.59 +1.8 2,507,321 + yafS JDDGAC_13570 0.59 +1.0 2,507,321 + yafS JDDGAC_13570 0.59 -0.1 2,507,322 - yafS JDDGAC_13570 0.59 -0.8 2,507,322 - yafS JDDGAC_13570 0.59 -2.6 2,507,322 - yafS JDDGAC_13570 0.59 +0.3 2,507,322 - yafS JDDGAC_13570 0.59 +0.4 2,507,322 - yafS JDDGAC_13570 0.59 +0.3 2,507,322 - yafS JDDGAC_13570 0.59 +0.0 2,507,477 + yafS JDDGAC_13570 0.81 +0.2 2,507,478 - yafS JDDGAC_13570 0.81 -0.3 2,507,649 - +0.0 2,507,657 + +0.7 2,507,664 - +0.1 2,507,664 - -0.3 2,507,685 + +0.3 2,507,752 + gloB JDDGAC_13575 0.14 -0.2 2,507,774 + gloB JDDGAC_13575 0.17 +0.3 2,507,871 + gloB JDDGAC_13575 0.29 +0.1 2,507,871 + gloB JDDGAC_13575 0.29 -0.5 2,507,872 - gloB JDDGAC_13575 0.29 -0.6 2,507,872 - gloB JDDGAC_13575 0.29 +0.3 2,507,894 + gloB JDDGAC_13575 0.32 -0.5 2,507,960 - gloB JDDGAC_13575 0.41 +0.2 2,507,960 - gloB JDDGAC_13575 0.41 +0.5 2,507,983 + gloB JDDGAC_13575 0.44 -2.1 2,507,990 + gloB JDDGAC_13575 0.45 +0.7 2,508,016 + gloB JDDGAC_13575 0.49 +0.3 2,508,123 - gloB JDDGAC_13575 0.63 +0.4 2,508,131 - gloB JDDGAC_13575 0.64 -0.6 2,508,149 + gloB JDDGAC_13575 0.66 -0.1 2,508,149 + gloB JDDGAC_13575 0.66 -0.3 2,508,149 + gloB JDDGAC_13575 0.66 -1.2 2,508,149 + gloB JDDGAC_13575 0.66 -1.7 2,508,150 - gloB JDDGAC_13575 0.66 -0.8 2,508,263 - gloB JDDGAC_13575 0.81 +0.2 2,508,263 - gloB JDDGAC_13575 0.81 +0.1 2,508,395 + -0.1 2,508,495 + -0.1
Or see this region's nucleotide sequence