Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppiD and hupB are separated by 191 nucleotides hupB and JDDGAC_11975 are separated by 208 nucleotides JDDGAC_11975 and clpX are separated by 187 nucleotides
JDDGAC_11965: ppiD - peptidylprolyl isomerase, at 2,195,100 to 2,196,971
ppiD
JDDGAC_11970: hupB - DNA-binding protein HU-beta, at 2,197,163 to 2,197,435
hupB
JDDGAC_11975: JDDGAC_11975 - Lon protease, at 2,197,644 to 2,199,998
_11975
JDDGAC_11985: clpX - ATP-dependent protease ATP-binding subunit ClpX, at 2,200,186 to 2,201,460
clpX
Position (kb)
2197
2198
2199
2200 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2196.752 kb on - strand, within ppiD at 2196.851 kb on - strand at 2196.895 kb on - strand at 2196.977 kb on - strand at 2197.101 kb on + strand at 2197.118 kb on + strand at 2197.118 kb on + strand at 2197.119 kb on - strand at 2197.164 kb on + strand at 2197.165 kb on - strand at 2197.189 kb on + strand at 2197.310 kb on + strand, within hupB at 2197.310 kb on + strand, within hupB at 2197.310 kb on + strand, within hupB at 2197.310 kb on + strand, within hupB at 2197.311 kb on - strand, within hupB at 2197.311 kb on - strand, within hupB at 2197.351 kb on + strand, within hupB at 2197.352 kb on - strand, within hupB at 2197.354 kb on + strand, within hupB at 2197.354 kb on + strand, within hupB at 2197.452 kb on - strand at 2197.452 kb on - strand at 2197.453 kb on + strand at 2197.454 kb on - strand at 2197.458 kb on + strand at 2197.459 kb on - strand at 2197.461 kb on - strand at 2197.500 kb on + strand at 2197.501 kb on - strand at 2197.501 kb on - strand at 2197.502 kb on + strand at 2197.502 kb on + strand at 2197.502 kb on + strand at 2197.502 kb on + strand at 2197.502 kb on + strand at 2197.523 kb on - strand at 2197.534 kb on + strand at 2197.537 kb on + strand at 2197.561 kb on + strand at 2197.562 kb on - strand at 2197.562 kb on - strand at 2197.562 kb on - strand at 2197.562 kb on - strand at 2197.562 kb on - strand at 2197.567 kb on - strand at 2197.599 kb on + strand at 2197.600 kb on - strand at 2197.623 kb on - strand at 2197.747 kb on - strand at 2197.747 kb on - strand at 2197.747 kb on - strand at 2198.122 kb on + strand, within JDDGAC_11975 at 2198.395 kb on + strand, within JDDGAC_11975 at 2198.395 kb on + strand, within JDDGAC_11975 at 2198.754 kb on - strand, within JDDGAC_11975 at 2198.807 kb on - strand, within JDDGAC_11975 at 2198.870 kb on - strand, within JDDGAC_11975 at 2198.990 kb on - strand, within JDDGAC_11975 at 2199.416 kb on - strand, within JDDGAC_11975 at 2199.483 kb on - strand, within JDDGAC_11975 at 2199.769 kb on - strand at 2199.891 kb on - strand at 2200.064 kb on - strand at 2200.064 kb on - strand at 2200.064 kb on - strand at 2200.064 kb on - strand at 2200.073 kb on + strand at 2200.074 kb on - strand at 2200.074 kb on - strand at 2200.074 kb on - strand at 2200.074 kb on - strand at 2200.082 kb on - strand at 2200.082 kb on - strand at 2200.082 kb on - strand at 2200.084 kb on - strand at 2200.165 kb on + strand at 2200.165 kb on + strand at 2200.165 kb on + strand at 2200.165 kb on + strand at 2200.166 kb on - strand at 2200.166 kb on - strand at 2200.307 kb on + strand at 2200.560 kb on + strand, within clpX at 2200.726 kb on - strand, within clpX at 2200.738 kb on - strand, within clpX at 2200.809 kb on + strand, within clpX at 2200.917 kb on - strand, within clpX
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 2,196,752 - ppiD JDDGAC_11965 0.88 +0.1 2,196,851 - -0.9 2,196,895 - -2.1 2,196,977 - +0.7 2,197,101 + -0.8 2,197,118 + -0.1 2,197,118 + -1.3 2,197,119 - -0.9 2,197,164 + +1.2 2,197,165 - -0.1 2,197,189 + -0.3 2,197,310 + hupB JDDGAC_11970 0.54 +1.7 2,197,310 + hupB JDDGAC_11970 0.54 +0.4 2,197,310 + hupB JDDGAC_11970 0.54 +0.4 2,197,310 + hupB JDDGAC_11970 0.54 +0.1 2,197,311 - hupB JDDGAC_11970 0.54 -0.2 2,197,311 - hupB JDDGAC_11970 0.54 +0.5 2,197,351 + hupB JDDGAC_11970 0.69 +0.2 2,197,352 - hupB JDDGAC_11970 0.69 +0.2 2,197,354 + hupB JDDGAC_11970 0.70 +0.1 2,197,354 + hupB JDDGAC_11970 0.70 +0.0 2,197,452 - -0.0 2,197,452 - -0.2 2,197,453 + -1.8 2,197,454 - -0.4 2,197,458 + +0.3 2,197,459 - +0.9 2,197,461 - +0.1 2,197,500 + +0.2 2,197,501 - +0.0 2,197,501 - +0.1 2,197,502 + -1.5 2,197,502 + +0.2 2,197,502 + +0.1 2,197,502 + -1.5 2,197,502 + -0.7 2,197,523 - -0.0 2,197,534 + +0.8 2,197,537 + -0.5 2,197,561 + -0.9 2,197,562 - -0.1 2,197,562 - +0.7 2,197,562 - +0.5 2,197,562 - -0.3 2,197,562 - -0.3 2,197,567 - -1.0 2,197,599 + -1.8 2,197,600 - -0.4 2,197,623 - -0.9 2,197,747 - -0.8 2,197,747 - -1.1 2,197,747 - -2.4 2,198,122 + JDDGAC_11975 0.20 -1.1 2,198,395 + JDDGAC_11975 0.32 -1.7 2,198,395 + JDDGAC_11975 0.32 -1.3 2,198,754 - JDDGAC_11975 0.47 -0.8 2,198,807 - JDDGAC_11975 0.49 +0.4 2,198,870 - JDDGAC_11975 0.52 +0.8 2,198,990 - JDDGAC_11975 0.57 -2.5 2,199,416 - JDDGAC_11975 0.75 -2.6 2,199,483 - JDDGAC_11975 0.78 -3.7 2,199,769 - -1.5 2,199,891 - -1.6 2,200,064 - -1.8 2,200,064 - -0.2 2,200,064 - -1.1 2,200,064 - +0.3 2,200,073 + -2.0 2,200,074 - -1.1 2,200,074 - -0.0 2,200,074 - -0.4 2,200,074 - +2.0 2,200,082 - +0.6 2,200,082 - -0.2 2,200,082 - +0.0 2,200,084 - +0.2 2,200,165 + +1.4 2,200,165 + -1.0 2,200,165 + -0.4 2,200,165 + -0.8 2,200,166 - -3.4 2,200,166 - -0.3 2,200,307 + -0.4 2,200,560 + clpX JDDGAC_11985 0.29 -1.2 2,200,726 - clpX JDDGAC_11985 0.42 -0.3 2,200,738 - clpX JDDGAC_11985 0.43 -0.2 2,200,809 + clpX JDDGAC_11985 0.49 -1.3 2,200,917 - clpX JDDGAC_11985 0.57 +0.1
Or see this region's nucleotide sequence