Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Lys_trna and Lys_trna are separated by 146 nucleotides Lys_trna and Val_trna are separated by 3 nucleotides Val_trna and Lys_trna are separated by 51 nucleotides Lys_trna and Val_trna are separated by 2 nucleotides Val_trna and Lys_trna are separated by 35 nucleotides Lys_trna and cpoB are separated by 164 nucleotides cpoB and pal are separated by 9 nucleotides pal and tolB are separated by 34 nucleotides
JDDGAC_10420: Lys_trna - tRNA-Lys(ttt), at 1,859,643 to 1,859,718
_trna
JDDGAC_10425: Lys_trna - tRNA-Lys(ttt), at 1,859,865 to 1,859,940
_trna
JDDGAC_10430: Val_trna - tRNA-Val(tac), at 1,859,944 to 1,860,019
_trna
JDDGAC_10435: Lys_trna - tRNA-Lys(ttt), at 1,860,071 to 1,860,146
_trna
JDDGAC_10440: Val_trna - tRNA-Val(tac), at 1,860,149 to 1,860,224
_trna
JDDGAC_10445: Lys_trna - tRNA-Lys(ttt), at 1,860,260 to 1,860,335
_trna
JDDGAC_10450: cpoB - cell division protein CpoB, at 1,860,500 to 1,861,291
cpoB
JDDGAC_10455: pal - peptidoglycan-associated lipoprotein Pal, at 1,861,301 to 1,861,822
pal
JDDGAC_10460: tolB - Tol-Pal system protein TolB, at 1,861,857 to 1,863,149
tolB
Position (kb) 1860
1861
1862 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1859.526 kb on - strand at 1859.539 kb on - strand at 1859.566 kb on + strand at 1859.639 kb on - strand at 1859.730 kb on - strand at 1859.920 kb on + strand, within Lys_trna at 1860.045 kb on - strand at 1860.045 kb on - strand at 1860.069 kb on - strand at 1860.240 kb on - strand at 1860.243 kb on - strand at 1860.428 kb on + strand at 1860.428 kb on + strand at 1860.429 kb on - strand at 1860.471 kb on + strand at 1860.501 kb on + strand at 1860.502 kb on - strand at 1860.533 kb on - strand at 1860.544 kb on + strand at 1860.544 kb on + strand at 1860.552 kb on + strand at 1860.552 kb on + strand at 1860.553 kb on - strand at 1860.556 kb on + strand at 1860.556 kb on + strand at 1860.557 kb on - strand at 1860.576 kb on - strand at 1860.629 kb on - strand, within cpoB at 1860.691 kb on - strand, within cpoB at 1860.720 kb on + strand, within cpoB at 1860.720 kb on + strand, within cpoB at 1860.726 kb on - strand, within cpoB at 1860.741 kb on + strand, within cpoB at 1861.145 kb on - strand, within cpoB at 1861.240 kb on + strand at 1861.240 kb on + strand at 1861.240 kb on + strand at 1861.250 kb on + strand at 1861.266 kb on - strand at 1861.282 kb on + strand at 1861.302 kb on + strand at 1861.306 kb on - strand at 1861.534 kb on + strand, within pal at 1861.591 kb on - strand, within pal at 1861.591 kb on - strand, within pal at 1861.609 kb on - strand, within pal at 1861.935 kb on - strand at 1862.047 kb on - strand, within tolB at 1862.171 kb on + strand, within tolB at 1862.172 kb on - strand, within tolB at 1862.172 kb on - strand, within tolB at 1862.172 kb on - strand, within tolB at 1862.172 kb on - strand, within tolB at 1862.172 kb on - strand, within tolB at 1862.205 kb on - strand, within tolB at 1862.207 kb on - strand, within tolB at 1862.238 kb on - strand, within tolB
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 1,859,526 - +1.0 1,859,539 - +0.2 1,859,566 + +1.1 1,859,639 - -0.1 1,859,730 - -0.1 1,859,920 + Lys_trna JDDGAC_10425 0.72 -1.2 1,860,045 - +0.9 1,860,045 - +0.3 1,860,069 - +0.4 1,860,240 - -0.8 1,860,243 - -0.3 1,860,428 + -1.0 1,860,428 + +0.6 1,860,429 - -1.9 1,860,471 + -1.3 1,860,501 + -0.9 1,860,502 - +0.3 1,860,533 - -0.8 1,860,544 + +0.2 1,860,544 + -0.0 1,860,552 + -0.5 1,860,552 + +0.7 1,860,553 - -1.1 1,860,556 + +0.6 1,860,556 + -1.1 1,860,557 - +0.3 1,860,576 - -1.6 1,860,629 - cpoB JDDGAC_10450 0.16 +0.3 1,860,691 - cpoB JDDGAC_10450 0.24 +1.2 1,860,720 + cpoB JDDGAC_10450 0.28 -0.1 1,860,720 + cpoB JDDGAC_10450 0.28 -0.2 1,860,726 - cpoB JDDGAC_10450 0.29 -0.1 1,860,741 + cpoB JDDGAC_10450 0.30 +0.5 1,861,145 - cpoB JDDGAC_10450 0.81 +0.5 1,861,240 + +0.9 1,861,240 + +0.4 1,861,240 + +0.2 1,861,250 + +0.4 1,861,266 - +0.1 1,861,282 + +0.2 1,861,302 + +0.3 1,861,306 - +0.9 1,861,534 + pal JDDGAC_10455 0.45 -1.2 1,861,591 - pal JDDGAC_10455 0.56 -1.1 1,861,591 - pal JDDGAC_10455 0.56 -1.0 1,861,609 - pal JDDGAC_10455 0.59 -1.6 1,861,935 - -2.1 1,862,047 - tolB JDDGAC_10460 0.15 -1.7 1,862,171 + tolB JDDGAC_10460 0.24 +2.2 1,862,172 - tolB JDDGAC_10460 0.24 -1.4 1,862,172 - tolB JDDGAC_10460 0.24 -0.3 1,862,172 - tolB JDDGAC_10460 0.24 -1.8 1,862,172 - tolB JDDGAC_10460 0.24 -1.6 1,862,172 - tolB JDDGAC_10460 0.24 -0.8 1,862,205 - tolB JDDGAC_10460 0.27 -2.0 1,862,207 - tolB JDDGAC_10460 0.27 -0.6 1,862,238 - tolB JDDGAC_10460 0.29 -2.0
Or see this region's nucleotide sequence