Strain Fitness in Escherichia coli ECRC98 around JDDGAC_03645

Experiment: WM_TP12

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuxaB and tam are separated by 277 nucleotidestam and lsrG are separated by 56 nucleotideslsrG and lsrF are separated by 23 nucleotides JDDGAC_03640: uxaB - tagaturonate reductase, at 605,932 to 607,383 uxaB JDDGAC_03645: tam - trans-aconitate 2-methyltransferase, at 607,661 to 607,927 tam JDDGAC_03650: lsrG - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 607,984 to 608,274 lsrG JDDGAC_03655: lsrF - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 608,298 to 609,173 lsrF Position (kb) 607 608Strain fitness (log2 ratio) -1 0 1at 606.664 kb on + strand, within uxaBat 606.665 kb on - strand, within uxaBat 606.842 kb on + strand, within uxaBat 606.895 kb on + strand, within uxaBat 607.384 kb on + strandat 607.576 kb on - strandat 607.650 kb on + strandat 607.827 kb on + strand, within tamat 607.828 kb on - strand, within tamat 607.902 kb on + strandat 607.905 kb on - strandat 608.108 kb on + strand, within lsrGat 608.318 kb on - strandat 608.327 kb on - strandat 608.558 kb on - strand, within lsrFat 608.617 kb on + strand, within lsrFat 608.920 kb on - strand, within lsrFat 608.923 kb on - strand, within lsrF

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP12
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606,664 + uxaB JDDGAC_03640 0.50 -1.5
606,665 - uxaB JDDGAC_03640 0.50 -1.2
606,842 + uxaB JDDGAC_03640 0.63 +0.2
606,895 + uxaB JDDGAC_03640 0.66 +0.1
607,384 + -0.1
607,576 - +0.5
607,650 + +0.8
607,827 + tam JDDGAC_03645 0.62 -1.5
607,828 - tam JDDGAC_03645 0.63 -0.1
607,902 + -0.5
607,905 - +1.3
608,108 + lsrG JDDGAC_03650 0.43 +0.3
608,318 - -1.1
608,327 - +1.6
608,558 - lsrF JDDGAC_03655 0.30 +0.3
608,617 + lsrF JDDGAC_03655 0.36 -0.6
608,920 - lsrF JDDGAC_03655 0.71 +1.1
608,923 - lsrF JDDGAC_03655 0.71 +0.3

Or see this region's nucleotide sequence