Experiment: WM_Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaJ and wzxC are separated by 1 nucleotides wzxC and wcaK are separated by 179 nucleotides
JDDGAC_28485: wcaJ - undecaprenyl-phosphate glucose phosphotransferase, at 5,462,398 to 5,463,792
wcaJ
JDDGAC_28490: wzxC - colanic acid undecaprenyl disphosphate flippase WzxC, at 5,463,794 to 5,465,272
wzxC
JDDGAC_28495: wcaK - colanic acid biosynthesis pyruvyl transferase WcaK, at 5,465,452 to 5,466,732
wcaK
Position (kb) 5463
5464
5465
5466 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5462.821 kb on + strand, within wcaJ at 5462.823 kb on + strand, within wcaJ at 5462.824 kb on - strand, within wcaJ at 5462.856 kb on - strand, within wcaJ at 5462.997 kb on + strand, within wcaJ at 5463.016 kb on - strand, within wcaJ at 5463.181 kb on + strand, within wcaJ at 5463.589 kb on + strand, within wcaJ at 5463.603 kb on + strand, within wcaJ at 5463.604 kb on - strand, within wcaJ at 5463.707 kb on - strand at 5463.802 kb on - strand at 5463.802 kb on - strand at 5463.958 kb on + strand, within wzxC at 5463.958 kb on + strand, within wzxC at 5463.982 kb on + strand, within wzxC at 5464.331 kb on - strand, within wzxC at 5464.464 kb on - strand, within wzxC at 5464.530 kb on - strand, within wzxC at 5464.810 kb on - strand, within wzxC at 5464.969 kb on + strand, within wzxC at 5465.034 kb on - strand, within wzxC at 5465.090 kb on + strand, within wzxC at 5465.090 kb on + strand, within wzxC at 5465.091 kb on - strand, within wzxC at 5465.151 kb on + strand at 5465.314 kb on + strand at 5465.324 kb on + strand at 5465.365 kb on + strand at 5465.460 kb on - strand at 5465.464 kb on - strand at 5465.520 kb on - strand at 5465.551 kb on - strand at 5465.714 kb on + strand, within wcaK at 5465.715 kb on - strand, within wcaK at 5465.715 kb on - strand, within wcaK at 5465.815 kb on - strand, within wcaK at 5465.815 kb on - strand, within wcaK at 5465.886 kb on + strand, within wcaK at 5466.115 kb on + strand, within wcaK at 5466.169 kb on - strand, within wcaK at 5466.223 kb on + strand, within wcaK
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas31 remove 5,462,821 + wcaJ JDDGAC_28485 0.30 +0.3 5,462,823 + wcaJ JDDGAC_28485 0.30 +0.4 5,462,824 - wcaJ JDDGAC_28485 0.31 +0.8 5,462,856 - wcaJ JDDGAC_28485 0.33 -0.7 5,462,997 + wcaJ JDDGAC_28485 0.43 +0.4 5,463,016 - wcaJ JDDGAC_28485 0.44 +1.1 5,463,181 + wcaJ JDDGAC_28485 0.56 +1.4 5,463,589 + wcaJ JDDGAC_28485 0.85 -0.8 5,463,603 + wcaJ JDDGAC_28485 0.86 +0.2 5,463,604 - wcaJ JDDGAC_28485 0.86 -1.3 5,463,707 - +2.0 5,463,802 - -0.2 5,463,802 - -0.0 5,463,958 + wzxC JDDGAC_28490 0.11 -0.8 5,463,958 + wzxC JDDGAC_28490 0.11 -0.0 5,463,982 + wzxC JDDGAC_28490 0.13 -0.7 5,464,331 - wzxC JDDGAC_28490 0.36 +0.2 5,464,464 - wzxC JDDGAC_28490 0.45 -0.9 5,464,530 - wzxC JDDGAC_28490 0.50 -0.4 5,464,810 - wzxC JDDGAC_28490 0.69 +0.5 5,464,969 + wzxC JDDGAC_28490 0.79 -0.3 5,465,034 - wzxC JDDGAC_28490 0.84 +1.2 5,465,090 + wzxC JDDGAC_28490 0.88 +0.0 5,465,090 + wzxC JDDGAC_28490 0.88 -0.4 5,465,091 - wzxC JDDGAC_28490 0.88 -1.2 5,465,151 + -1.4 5,465,314 + +0.1 5,465,324 + +0.4 5,465,365 + +0.7 5,465,460 - -0.3 5,465,464 - -1.9 5,465,520 - -1.3 5,465,551 - -0.1 5,465,714 + wcaK JDDGAC_28495 0.20 -1.6 5,465,715 - wcaK JDDGAC_28495 0.21 +0.7 5,465,715 - wcaK JDDGAC_28495 0.21 -0.7 5,465,815 - wcaK JDDGAC_28495 0.28 +1.5 5,465,815 - wcaK JDDGAC_28495 0.28 +0.3 5,465,886 + wcaK JDDGAC_28495 0.34 -0.3 5,466,115 + wcaK JDDGAC_28495 0.52 +0.0 5,466,169 - wcaK JDDGAC_28495 0.56 +0.9 5,466,223 + wcaK JDDGAC_28495 0.60 -0.2
Or see this region's nucleotide sequence