Experiment: WM_Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pheT and JDDGAC_01665 are separated by 4 nucleotides JDDGAC_01665 and btuC are separated by 100 nucleotides btuC and btuE are separated by 62 nucleotides btuE and btuD overlap by 1 nucleotides
JDDGAC_01660: pheT - phenylalanine--tRNA ligase subunit beta, at 291,582 to 293,969
pheT
JDDGAC_01665: JDDGAC_01665 - Integration host factor subunit alpha, at 293,974 to 294,273
_01665
JDDGAC_01675: btuC - vitamin B12 ABC transporter permease BtuC, at 294,374 to 295,354
btuC
JDDGAC_01680: btuE - bifunctional thioredoxin/glutathione peroxidase, at 295,417 to 295,968
btuE
JDDGAC_01685: btuD - vitamin B12 ABC transporter ATP-binding protein BtuD, at 295,968 to 296,717
btuD
Position (kb) 294
295
296 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 294.551 kb on - strand, within btuC at 294.841 kb on + strand, within btuC at 294.849 kb on + strand, within btuC at 294.850 kb on - strand, within btuC at 294.863 kb on - strand, within btuC at 294.866 kb on + strand, within btuC at 294.888 kb on + strand, within btuC at 295.010 kb on - strand, within btuC at 295.159 kb on + strand, within btuC at 295.197 kb on + strand, within btuC at 295.286 kb on - strand at 295.377 kb on + strand at 295.544 kb on - strand, within btuE at 295.660 kb on + strand, within btuE at 295.660 kb on + strand, within btuE at 295.665 kb on + strand, within btuE at 295.966 kb on + strand at 295.973 kb on + strand at 296.088 kb on - strand, within btuD at 296.092 kb on + strand, within btuD at 296.092 kb on + strand, within btuD
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas31 remove 294,551 - btuC JDDGAC_01675 0.18 +2.2 294,841 + btuC JDDGAC_01675 0.48 -1.3 294,849 + btuC JDDGAC_01675 0.48 -1.6 294,850 - btuC JDDGAC_01675 0.49 +0.0 294,863 - btuC JDDGAC_01675 0.50 -2.2 294,866 + btuC JDDGAC_01675 0.50 -0.6 294,888 + btuC JDDGAC_01675 0.52 +0.1 295,010 - btuC JDDGAC_01675 0.65 +1.1 295,159 + btuC JDDGAC_01675 0.80 +0.1 295,197 + btuC JDDGAC_01675 0.84 -1.9 295,286 - -1.2 295,377 + +0.3 295,544 - btuE JDDGAC_01680 0.23 -0.3 295,660 + btuE JDDGAC_01680 0.44 -0.6 295,660 + btuE JDDGAC_01680 0.44 +1.0 295,665 + btuE JDDGAC_01680 0.45 +0.9 295,966 + -1.8 295,973 + -0.5 296,088 - btuD JDDGAC_01685 0.16 -3.1 296,092 + btuD JDDGAC_01685 0.17 -0.4 296,092 + btuD JDDGAC_01685 0.17 -1.1
Or see this region's nucleotide sequence