Experiment: WM_Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amyA and fliT are separated by 77 nucleotides fliT and fliS overlap by 1 nucleotides fliS and fliD are separated by 24 nucleotides
JDDGAC_00345: amyA - alpha-amylase, at 59,071 to 60,558
amyA
JDDGAC_00350: fliT - flagella biosynthesis regulatory protein FliT, at 60,636 to 61,001
fliT
JDDGAC_00355: fliS - flagellar export chaperone FliS, at 61,001 to 61,411
fliS
JDDGAC_00360: fliD - flagellar filament capping protein FliD, at 61,436 to 62,833
fliD
Position (kb) 60
61
62 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 59.841 kb on + strand, within amyA at 60.111 kb on - strand, within amyA at 60.254 kb on - strand, within amyA at 60.282 kb on + strand, within amyA at 60.333 kb on + strand, within amyA at 60.334 kb on - strand, within amyA at 60.367 kb on + strand, within amyA at 60.376 kb on + strand, within amyA at 60.424 kb on - strand at 60.425 kb on + strand at 60.425 kb on + strand at 60.425 kb on + strand at 60.447 kb on + strand at 60.538 kb on + strand at 60.538 kb on + strand at 60.539 kb on - strand at 60.586 kb on - strand at 60.586 kb on - strand at 60.760 kb on - strand, within fliT at 60.768 kb on + strand, within fliT at 60.769 kb on - strand, within fliT at 60.879 kb on - strand, within fliT at 60.884 kb on - strand, within fliT at 60.884 kb on - strand, within fliT at 60.979 kb on + strand at 60.982 kb on - strand at 60.982 kb on - strand at 61.060 kb on + strand, within fliS at 61.141 kb on - strand, within fliS at 61.141 kb on - strand, within fliS at 61.141 kb on - strand, within fliS at 61.141 kb on - strand, within fliS at 61.141 kb on - strand, within fliS at 61.141 kb on - strand, within fliS at 61.276 kb on + strand, within fliS at 61.319 kb on + strand, within fliS at 61.320 kb on - strand, within fliS at 61.431 kb on - strand at 61.447 kb on + strand at 61.448 kb on - strand at 61.448 kb on - strand at 61.472 kb on + strand at 61.476 kb on + strand at 61.477 kb on - strand at 61.477 kb on - strand at 61.536 kb on + strand at 61.724 kb on + strand, within fliD at 61.725 kb on - strand, within fliD at 61.808 kb on - strand, within fliD at 61.849 kb on + strand, within fliD at 61.982 kb on - strand, within fliD at 61.982 kb on - strand, within fliD at 61.986 kb on - strand, within fliD
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas31 remove 59,841 + amyA JDDGAC_00345 0.52 -0.5 60,111 - amyA JDDGAC_00345 0.70 -0.1 60,254 - amyA JDDGAC_00345 0.80 +0.5 60,282 + amyA JDDGAC_00345 0.81 -0.1 60,333 + amyA JDDGAC_00345 0.85 +0.9 60,334 - amyA JDDGAC_00345 0.85 +0.5 60,367 + amyA JDDGAC_00345 0.87 -0.3 60,376 + amyA JDDGAC_00345 0.88 -0.1 60,424 - +0.4 60,425 + -1.6 60,425 + -0.6 60,425 + +0.5 60,447 + +0.4 60,538 + +0.6 60,538 + -2.3 60,539 - +0.1 60,586 - -0.1 60,586 - +0.6 60,760 - fliT JDDGAC_00350 0.34 +0.2 60,768 + fliT JDDGAC_00350 0.36 -2.9 60,769 - fliT JDDGAC_00350 0.36 +0.9 60,879 - fliT JDDGAC_00350 0.66 -1.9 60,884 - fliT JDDGAC_00350 0.68 +1.7 60,884 - fliT JDDGAC_00350 0.68 -0.3 60,979 + -0.4 60,982 - +0.4 60,982 - -0.1 61,060 + fliS JDDGAC_00355 0.14 -2.4 61,141 - fliS JDDGAC_00355 0.34 +0.6 61,141 - fliS JDDGAC_00355 0.34 +0.9 61,141 - fliS JDDGAC_00355 0.34 +0.1 61,141 - fliS JDDGAC_00355 0.34 -1.6 61,141 - fliS JDDGAC_00355 0.34 +0.9 61,141 - fliS JDDGAC_00355 0.34 +0.9 61,276 + fliS JDDGAC_00355 0.67 +0.4 61,319 + fliS JDDGAC_00355 0.77 +0.1 61,320 - fliS JDDGAC_00355 0.78 +2.4 61,431 - -0.5 61,447 + -0.9 61,448 - +0.3 61,448 - -2.9 61,472 + -0.8 61,476 + +0.1 61,477 - -0.6 61,477 - +0.1 61,536 + -1.0 61,724 + fliD JDDGAC_00360 0.21 +0.3 61,725 - fliD JDDGAC_00360 0.21 +0.5 61,808 - fliD JDDGAC_00360 0.27 +0.7 61,849 + fliD JDDGAC_00360 0.30 +0.6 61,982 - fliD JDDGAC_00360 0.39 +1.0 61,982 - fliD JDDGAC_00360 0.39 -0.9 61,986 - fliD JDDGAC_00360 0.39 +0.2
Or see this region's nucleotide sequence