Experiment: WM_Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt guaB and guaA are separated by 68 nucleotides guaA and JDDGAC_25445 are separated by 192 nucleotides
JDDGAC_25435: guaB - IMP dehydrogenase, at 4,870,528 to 4,871,994
guaB
JDDGAC_25440: guaA - glutamine-hydrolyzing GMP synthase, at 4,872,063 to 4,873,640
guaA
JDDGAC_25445: JDDGAC_25445 - integrase, at 4,873,833 to 4,875,083
_25445
Position (kb)
4872
4873
4874 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4871.160 kb on + strand, within guaB at 4871.325 kb on + strand, within guaB at 4871.436 kb on + strand, within guaB at 4871.437 kb on - strand, within guaB at 4871.437 kb on - strand, within guaB at 4871.443 kb on - strand, within guaB at 4871.452 kb on - strand, within guaB at 4871.465 kb on + strand, within guaB at 4871.523 kb on + strand, within guaB at 4871.606 kb on + strand, within guaB at 4871.606 kb on + strand, within guaB at 4871.656 kb on + strand, within guaB at 4871.657 kb on - strand, within guaB at 4871.672 kb on - strand, within guaB at 4871.688 kb on + strand, within guaB at 4871.789 kb on + strand, within guaB at 4871.789 kb on + strand, within guaB at 4871.794 kb on + strand, within guaB at 4871.795 kb on - strand, within guaB at 4871.795 kb on - strand, within guaB at 4871.796 kb on + strand, within guaB at 4871.797 kb on - strand, within guaB at 4871.872 kb on - strand at 4871.872 kb on - strand at 4871.906 kb on + strand at 4871.907 kb on - strand at 4871.958 kb on + strand at 4871.958 kb on + strand at 4871.978 kb on - strand at 4872.026 kb on + strand at 4872.031 kb on + strand at 4872.031 kb on + strand at 4872.032 kb on - strand at 4872.117 kb on + strand at 4872.248 kb on + strand, within guaA at 4872.248 kb on + strand, within guaA at 4872.409 kb on + strand, within guaA at 4873.015 kb on + strand, within guaA at 4873.370 kb on + strand, within guaA at 4873.454 kb on + strand, within guaA at 4873.458 kb on + strand, within guaA at 4873.778 kb on - strand at 4873.788 kb on + strand at 4873.790 kb on + strand at 4873.909 kb on - strand at 4873.912 kb on + strand at 4873.923 kb on - strand at 4873.948 kb on - strand at 4874.148 kb on + strand, within JDDGAC_25445 at 4874.149 kb on - strand, within JDDGAC_25445 at 4874.149 kb on - strand, within JDDGAC_25445 at 4874.215 kb on + strand, within JDDGAC_25445 at 4874.215 kb on + strand, within JDDGAC_25445 at 4874.344 kb on + strand, within JDDGAC_25445 at 4874.344 kb on + strand, within JDDGAC_25445 at 4874.344 kb on + strand, within JDDGAC_25445 at 4874.345 kb on - strand, within JDDGAC_25445 at 4874.346 kb on + strand, within JDDGAC_25445 at 4874.347 kb on - strand, within JDDGAC_25445 at 4874.420 kb on - strand, within JDDGAC_25445 at 4874.518 kb on + strand, within JDDGAC_25445 at 4874.532 kb on - strand, within JDDGAC_25445 at 4874.537 kb on + strand, within JDDGAC_25445 at 4874.538 kb on - strand, within JDDGAC_25445
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas31 remove 4,871,160 + guaB JDDGAC_25435 0.43 -2.2 4,871,325 + guaB JDDGAC_25435 0.54 -2.7 4,871,436 + guaB JDDGAC_25435 0.62 -4.3 4,871,437 - guaB JDDGAC_25435 0.62 -3.4 4,871,437 - guaB JDDGAC_25435 0.62 -2.2 4,871,443 - guaB JDDGAC_25435 0.62 -2.3 4,871,452 - guaB JDDGAC_25435 0.63 -2.9 4,871,465 + guaB JDDGAC_25435 0.64 -1.2 4,871,523 + guaB JDDGAC_25435 0.68 -3.1 4,871,606 + guaB JDDGAC_25435 0.73 -3.1 4,871,606 + guaB JDDGAC_25435 0.73 -2.4 4,871,656 + guaB JDDGAC_25435 0.77 -5.1 4,871,657 - guaB JDDGAC_25435 0.77 -1.8 4,871,672 - guaB JDDGAC_25435 0.78 -1.0 4,871,688 + guaB JDDGAC_25435 0.79 -3.6 4,871,789 + guaB JDDGAC_25435 0.86 -1.8 4,871,789 + guaB JDDGAC_25435 0.86 -4.2 4,871,794 + guaB JDDGAC_25435 0.86 -3.3 4,871,795 - guaB JDDGAC_25435 0.86 -1.8 4,871,795 - guaB JDDGAC_25435 0.86 -0.2 4,871,796 + guaB JDDGAC_25435 0.86 -4.6 4,871,797 - guaB JDDGAC_25435 0.87 -0.4 4,871,872 - -0.3 4,871,872 - -3.3 4,871,906 + -4.1 4,871,907 - -1.6 4,871,958 + -0.3 4,871,958 + -0.6 4,871,978 - -3.9 4,872,026 + +3.0 4,872,031 + +0.1 4,872,031 + +0.4 4,872,032 - -2.1 4,872,117 + -4.2 4,872,248 + guaA JDDGAC_25440 0.12 -0.9 4,872,248 + guaA JDDGAC_25440 0.12 +0.6 4,872,409 + guaA JDDGAC_25440 0.22 -1.2 4,873,015 + guaA JDDGAC_25440 0.60 -0.4 4,873,370 + guaA JDDGAC_25440 0.83 -1.0 4,873,454 + guaA JDDGAC_25440 0.88 -1.0 4,873,458 + guaA JDDGAC_25440 0.88 -1.4 4,873,778 - -0.9 4,873,788 + -0.7 4,873,790 + -2.7 4,873,909 - -0.4 4,873,912 + +0.6 4,873,923 - -0.9 4,873,948 - -1.7 4,874,148 + JDDGAC_25445 0.25 +1.4 4,874,149 - JDDGAC_25445 0.25 +0.8 4,874,149 - JDDGAC_25445 0.25 +0.8 4,874,215 + JDDGAC_25445 0.31 +0.4 4,874,215 + JDDGAC_25445 0.31 -1.8 4,874,344 + JDDGAC_25445 0.41 +0.1 4,874,344 + JDDGAC_25445 0.41 -0.1 4,874,344 + JDDGAC_25445 0.41 -0.8 4,874,345 - JDDGAC_25445 0.41 -0.8 4,874,346 + JDDGAC_25445 0.41 +0.8 4,874,347 - JDDGAC_25445 0.41 +0.1 4,874,420 - JDDGAC_25445 0.47 +1.4 4,874,518 + JDDGAC_25445 0.55 +0.6 4,874,532 - JDDGAC_25445 0.56 +2.1 4,874,537 + JDDGAC_25445 0.56 +0.3 4,874,538 - JDDGAC_25445 0.56 +0.3
Or see this region's nucleotide sequence