Experiment: WM_Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt xseA and guaB are separated by 161 nucleotides guaB and guaA are separated by 68 nucleotides
JDDGAC_25430: xseA - exodeoxyribonuclease VII large subunit, at 4,868,996 to 4,870,366
xseA
JDDGAC_25435: guaB - IMP dehydrogenase, at 4,870,528 to 4,871,994
guaB
JDDGAC_25440: guaA - glutamine-hydrolyzing GMP synthase, at 4,872,063 to 4,873,640
guaA
Position (kb)
4870
4871
4872 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4869.901 kb on + strand, within xseA at 4869.909 kb on - strand, within xseA at 4869.909 kb on - strand, within xseA at 4870.064 kb on - strand, within xseA at 4870.077 kb on - strand, within xseA at 4870.101 kb on + strand, within xseA at 4870.112 kb on - strand, within xseA at 4870.112 kb on - strand, within xseA at 4870.224 kb on + strand, within xseA at 4870.331 kb on - strand at 4870.331 kb on - strand at 4870.361 kb on + strand at 4870.361 kb on + strand at 4870.362 kb on - strand at 4870.410 kb on - strand at 4870.481 kb on + strand at 4870.482 kb on - strand at 4870.499 kb on + strand at 4870.500 kb on - strand at 4870.532 kb on + strand at 4870.532 kb on + strand at 4870.533 kb on - strand at 4870.536 kb on + strand at 4870.537 kb on - strand at 4870.590 kb on + strand at 4870.638 kb on + strand at 4870.927 kb on - strand, within guaB at 4870.982 kb on - strand, within guaB at 4871.160 kb on + strand, within guaB at 4871.325 kb on + strand, within guaB at 4871.436 kb on + strand, within guaB at 4871.437 kb on - strand, within guaB at 4871.437 kb on - strand, within guaB at 4871.443 kb on - strand, within guaB at 4871.452 kb on - strand, within guaB at 4871.465 kb on + strand, within guaB at 4871.523 kb on + strand, within guaB at 4871.606 kb on + strand, within guaB at 4871.606 kb on + strand, within guaB at 4871.656 kb on + strand, within guaB at 4871.657 kb on - strand, within guaB at 4871.672 kb on - strand, within guaB at 4871.688 kb on + strand, within guaB at 4871.789 kb on + strand, within guaB at 4871.789 kb on + strand, within guaB at 4871.794 kb on + strand, within guaB at 4871.795 kb on - strand, within guaB at 4871.795 kb on - strand, within guaB at 4871.796 kb on + strand, within guaB at 4871.797 kb on - strand, within guaB at 4871.872 kb on - strand at 4871.872 kb on - strand at 4871.906 kb on + strand at 4871.907 kb on - strand at 4871.958 kb on + strand at 4871.958 kb on + strand at 4871.978 kb on - strand at 4872.026 kb on + strand at 4872.031 kb on + strand at 4872.031 kb on + strand at 4872.032 kb on - strand at 4872.117 kb on + strand at 4872.248 kb on + strand, within guaA at 4872.248 kb on + strand, within guaA at 4872.409 kb on + strand, within guaA
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas31 remove 4,869,901 + xseA JDDGAC_25430 0.66 -0.9 4,869,909 - xseA JDDGAC_25430 0.67 -0.4 4,869,909 - xseA JDDGAC_25430 0.67 -0.3 4,870,064 - xseA JDDGAC_25430 0.78 -0.7 4,870,077 - xseA JDDGAC_25430 0.79 +0.1 4,870,101 + xseA JDDGAC_25430 0.81 +1.4 4,870,112 - xseA JDDGAC_25430 0.81 -0.5 4,870,112 - xseA JDDGAC_25430 0.81 -1.0 4,870,224 + xseA JDDGAC_25430 0.90 +0.7 4,870,331 - -0.7 4,870,331 - -0.9 4,870,361 + +0.5 4,870,361 + -0.2 4,870,362 - +0.4 4,870,410 - -1.3 4,870,481 + -4.9 4,870,482 - -2.4 4,870,499 + -1.0 4,870,500 - +0.6 4,870,532 + -3.0 4,870,532 + -3.1 4,870,533 - -3.6 4,870,536 + -3.2 4,870,537 - -3.5 4,870,590 + -4.3 4,870,638 + -4.3 4,870,927 - guaB JDDGAC_25435 0.27 -2.2 4,870,982 - guaB JDDGAC_25435 0.31 -2.2 4,871,160 + guaB JDDGAC_25435 0.43 -2.2 4,871,325 + guaB JDDGAC_25435 0.54 -2.7 4,871,436 + guaB JDDGAC_25435 0.62 -4.3 4,871,437 - guaB JDDGAC_25435 0.62 -3.4 4,871,437 - guaB JDDGAC_25435 0.62 -2.2 4,871,443 - guaB JDDGAC_25435 0.62 -2.3 4,871,452 - guaB JDDGAC_25435 0.63 -2.9 4,871,465 + guaB JDDGAC_25435 0.64 -1.2 4,871,523 + guaB JDDGAC_25435 0.68 -3.1 4,871,606 + guaB JDDGAC_25435 0.73 -3.1 4,871,606 + guaB JDDGAC_25435 0.73 -2.4 4,871,656 + guaB JDDGAC_25435 0.77 -5.1 4,871,657 - guaB JDDGAC_25435 0.77 -1.8 4,871,672 - guaB JDDGAC_25435 0.78 -1.0 4,871,688 + guaB JDDGAC_25435 0.79 -3.6 4,871,789 + guaB JDDGAC_25435 0.86 -1.8 4,871,789 + guaB JDDGAC_25435 0.86 -4.2 4,871,794 + guaB JDDGAC_25435 0.86 -3.3 4,871,795 - guaB JDDGAC_25435 0.86 -1.8 4,871,795 - guaB JDDGAC_25435 0.86 -0.2 4,871,796 + guaB JDDGAC_25435 0.86 -4.6 4,871,797 - guaB JDDGAC_25435 0.87 -0.4 4,871,872 - -0.3 4,871,872 - -3.3 4,871,906 + -4.1 4,871,907 - -1.6 4,871,958 + -0.3 4,871,958 + -0.6 4,871,978 - -3.9 4,872,026 + +3.0 4,872,031 + +0.1 4,872,031 + +0.4 4,872,032 - -2.1 4,872,117 + -4.2 4,872,248 + guaA JDDGAC_25440 0.12 -0.9 4,872,248 + guaA JDDGAC_25440 0.12 +0.6 4,872,409 + guaA JDDGAC_25440 0.22 -1.2
Or see this region's nucleotide sequence