Strain Fitness in Escherichia coli ECRC98 around JDDGAC_25410

Experiment: WM_Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisS and yfgM are separated by 17 nucleotidesyfgM and bamB are separated by 10 nucleotides JDDGAC_25405: hisS - histidine--tRNA ligase, at 4,864,024 to 4,865,298 hisS JDDGAC_25410: yfgM - ancillary SecYEG translocon subunit YfgM, at 4,865,316 to 4,865,936 yfgM JDDGAC_25415: bamB - outer membrane protein assembly factor BamB, at 4,865,947 to 4,867,125 bamB Position (kb) 4865 4866Strain fitness (log2 ratio) -2 -1 0 1at 4865.267 kb on + strandat 4865.626 kb on + strand, within yfgMat 4865.762 kb on + strand, within yfgMat 4865.834 kb on + strand, within yfgMat 4865.882 kb on + strandat 4865.882 kb on + strandat 4865.918 kb on + strandat 4866.035 kb on + strandat 4866.163 kb on + strand, within bamBat 4866.185 kb on + strand, within bamBat 4866.276 kb on - strand, within bamBat 4866.381 kb on + strand, within bamBat 4866.381 kb on + strand, within bamBat 4866.381 kb on + strand, within bamBat 4866.458 kb on + strand, within bamBat 4866.639 kb on + strand, within bamBat 4866.640 kb on - strand, within bamBat 4866.777 kb on + strand, within bamB

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas31
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4,865,267 + +0.3
4,865,626 + yfgM JDDGAC_25410 0.50 -0.9
4,865,762 + yfgM JDDGAC_25410 0.72 -1.4
4,865,834 + yfgM JDDGAC_25410 0.83 -1.8
4,865,882 + +0.5
4,865,882 + -0.2
4,865,918 + -1.3
4,866,035 + -0.2
4,866,163 + bamB JDDGAC_25415 0.18 -0.9
4,866,185 + bamB JDDGAC_25415 0.20 -0.7
4,866,276 - bamB JDDGAC_25415 0.28 +0.1
4,866,381 + bamB JDDGAC_25415 0.37 -0.7
4,866,381 + bamB JDDGAC_25415 0.37 -1.2
4,866,381 + bamB JDDGAC_25415 0.37 -2.4
4,866,458 + bamB JDDGAC_25415 0.43 -0.3
4,866,639 + bamB JDDGAC_25415 0.59 -0.3
4,866,640 - bamB JDDGAC_25415 0.59 -1.2
4,866,777 + bamB JDDGAC_25415 0.70 +1.5

Or see this region's nucleotide sequence