Strain Fitness in Escherichia coli ECRC98 around JDDGAC_23420

Experiment: WM_Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsctN and JDDGAC_23410 overlap by 11 nucleotidesJDDGAC_23410 and JDDGAC_23415 overlap by 23 nucleotidesJDDGAC_23415 and JDDGAC_23420 are separated by 72 nucleotidesJDDGAC_23420 and fliN overlap by 41 nucleotidesfliN and escR overlap by 11 nucleotides JDDGAC_23405: sctN - type III secretion system ATPase SctN, at 4,488,792 to 4,490,084 sctN JDDGAC_23410: JDDGAC_23410 - type III secretion protein, at 4,490,074 to 4,490,532 _23410 JDDGAC_23415: JDDGAC_23415 - Type III secretion protein, at 4,490,510 to 4,490,743 _23415 JDDGAC_23420: JDDGAC_23420 - type III secretion protein, at 4,490,816 to 4,491,385 _23420 JDDGAC_23425: fliN - YscQ/HrcQ family type III secretion apparatus protein, at 4,491,345 to 4,492,331 fliN JDDGAC_23430: escR - EscR/YscR/HrcR family type III secretion system export apparatus protein, at 4,492,321 to 4,492,986 escR Position (kb) 4490 4491 4492Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4489.877 kb on + strand, within sctNat 4489.878 kb on - strand, within sctNat 4489.921 kb on + strand, within sctNat 4489.922 kb on - strand, within sctNat 4489.939 kb on + strand, within sctNat 4490.098 kb on - strandat 4490.098 kb on - strandat 4491.173 kb on - strand, within JDDGAC_23420at 4491.193 kb on + strand, within JDDGAC_23420at 4491.268 kb on + strand, within JDDGAC_23420at 4491.776 kb on + strand, within fliN

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas31
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4,489,877 + sctN JDDGAC_23405 0.84 +0.2
4,489,878 - sctN JDDGAC_23405 0.84 -0.1
4,489,921 + sctN JDDGAC_23405 0.87 +0.3
4,489,922 - sctN JDDGAC_23405 0.87 -1.1
4,489,939 + sctN JDDGAC_23405 0.89 +0.5
4,490,098 - +1.3
4,490,098 - +0.2
4,491,173 - JDDGAC_23420 0.63 -0.0
4,491,193 + JDDGAC_23420 0.66 -1.7
4,491,268 + JDDGAC_23420 0.79 -3.8
4,491,776 + fliN JDDGAC_23425 0.44 -1.6

Or see this region's nucleotide sequence