Experiment: WM_Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sctN and JDDGAC_23410 overlap by 11 nucleotides JDDGAC_23410 and JDDGAC_23415 overlap by 23 nucleotides JDDGAC_23415 and JDDGAC_23420 are separated by 72 nucleotides JDDGAC_23420 and fliN overlap by 41 nucleotides fliN and escR overlap by 11 nucleotides
JDDGAC_23405: sctN - type III secretion system ATPase SctN, at 4,488,792 to 4,490,084
sctN
JDDGAC_23410: JDDGAC_23410 - type III secretion protein, at 4,490,074 to 4,490,532
_23410
JDDGAC_23415: JDDGAC_23415 - Type III secretion protein, at 4,490,510 to 4,490,743
_23415
JDDGAC_23420: JDDGAC_23420 - type III secretion protein, at 4,490,816 to 4,491,385
_23420
JDDGAC_23425: fliN - YscQ/HrcQ family type III secretion apparatus protein, at 4,491,345 to 4,492,331
fliN
JDDGAC_23430: escR - EscR/YscR/HrcR family type III secretion system export apparatus protein, at 4,492,321 to 4,492,986
escR
Position (kb)
4490
4491
4492 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4489.877 kb on + strand, within sctN at 4489.878 kb on - strand, within sctN at 4489.921 kb on + strand, within sctN at 4489.922 kb on - strand, within sctN at 4489.939 kb on + strand, within sctN at 4490.098 kb on - strand at 4490.098 kb on - strand at 4491.173 kb on - strand, within JDDGAC_23420 at 4491.193 kb on + strand, within JDDGAC_23420 at 4491.268 kb on + strand, within JDDGAC_23420 at 4491.776 kb on + strand, within fliN
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas31 remove 4,489,877 + sctN JDDGAC_23405 0.84 +0.2 4,489,878 - sctN JDDGAC_23405 0.84 -0.1 4,489,921 + sctN JDDGAC_23405 0.87 +0.3 4,489,922 - sctN JDDGAC_23405 0.87 -1.1 4,489,939 + sctN JDDGAC_23405 0.89 +0.5 4,490,098 - +1.3 4,490,098 - +0.2 4,491,173 - JDDGAC_23420 0.63 -0.0 4,491,193 + JDDGAC_23420 0.66 -1.7 4,491,268 + JDDGAC_23420 0.79 -3.8 4,491,776 + fliN JDDGAC_23425 0.44 -1.6
Or see this region's nucleotide sequence