Strain Fitness in Escherichia coli ECRC98 around JDDGAC_19300

Experiment: WM_Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwaaO and rfaY are separated by 25 nucleotidesrfaY and rfaJ are separated by 24 nucleotidesrfaJ and rfaB are separated by 8 nucleotides JDDGAC_19290: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 3,669,035 to 3,670,042 waaO JDDGAC_19295: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 3,670,068 to 3,670,775 rfaY JDDGAC_19300: rfaJ - glucosyltransferase, at 3,670,800 to 3,671,813 rfaJ JDDGAC_19305: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 3,671,822 to 3,672,964 rfaB Position (kb) 3670 3671 3672Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3669.819 kb on + strand, within waaOat 3669.820 kb on - strand, within waaOat 3669.824 kb on + strand, within waaOat 3669.925 kb on - strand, within waaOat 3670.067 kb on + strandat 3670.074 kb on + strandat 3670.384 kb on + strand, within rfaYat 3670.757 kb on - strandat 3670.984 kb on + strand, within rfaJat 3671.010 kb on + strand, within rfaJat 3671.179 kb on + strand, within rfaJat 3671.310 kb on - strand, within rfaJat 3671.513 kb on + strand, within rfaJat 3671.593 kb on + strand, within rfaJat 3671.598 kb on + strand, within rfaJat 3671.637 kb on + strand, within rfaJat 3671.904 kb on - strandat 3672.072 kb on - strand, within rfaBat 3672.198 kb on + strand, within rfaBat 3672.386 kb on - strand, within rfaBat 3672.601 kb on + strand, within rfaBat 3672.601 kb on + strand, within rfaBat 3672.601 kb on + strand, within rfaBat 3672.602 kb on - strand, within rfaBat 3672.607 kb on - strand, within rfaBat 3672.712 kb on + strand, within rfaBat 3672.779 kb on - strand, within rfaBat 3672.806 kb on - strand, within rfaB

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas31
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3,669,819 + waaO JDDGAC_19290 0.78 -1.1
3,669,820 - waaO JDDGAC_19290 0.78 -2.9
3,669,824 + waaO JDDGAC_19290 0.78 -2.1
3,669,925 - waaO JDDGAC_19290 0.88 -1.7
3,670,067 + +1.4
3,670,074 + +0.1
3,670,384 + rfaY JDDGAC_19295 0.45 +0.7
3,670,757 - -1.9
3,670,984 + rfaJ JDDGAC_19300 0.18 -2.5
3,671,010 + rfaJ JDDGAC_19300 0.21 -2.3
3,671,179 + rfaJ JDDGAC_19300 0.37 -1.7
3,671,310 - rfaJ JDDGAC_19300 0.50 -2.6
3,671,513 + rfaJ JDDGAC_19300 0.70 -3.0
3,671,593 + rfaJ JDDGAC_19300 0.78 -1.4
3,671,598 + rfaJ JDDGAC_19300 0.79 -1.6
3,671,637 + rfaJ JDDGAC_19300 0.83 -1.9
3,671,904 - -3.9
3,672,072 - rfaB JDDGAC_19305 0.22 -1.7
3,672,198 + rfaB JDDGAC_19305 0.33 -1.4
3,672,386 - rfaB JDDGAC_19305 0.49 +0.1
3,672,601 + rfaB JDDGAC_19305 0.68 -3.4
3,672,601 + rfaB JDDGAC_19305 0.68 -2.9
3,672,601 + rfaB JDDGAC_19305 0.68 -1.6
3,672,602 - rfaB JDDGAC_19305 0.68 -2.7
3,672,607 - rfaB JDDGAC_19305 0.69 -1.7
3,672,712 + rfaB JDDGAC_19305 0.78 -0.0
3,672,779 - rfaB JDDGAC_19305 0.84 -0.7
3,672,806 - rfaB JDDGAC_19305 0.86 -4.6

Or see this region's nucleotide sequence