Experiment: WM_Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt waaO and rfaY are separated by 25 nucleotides rfaY and rfaJ are separated by 24 nucleotides rfaJ and rfaB are separated by 8 nucleotides
JDDGAC_19290: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 3,669,035 to 3,670,042
waaO
JDDGAC_19295: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 3,670,068 to 3,670,775
rfaY
JDDGAC_19300: rfaJ - glucosyltransferase, at 3,670,800 to 3,671,813
rfaJ
JDDGAC_19305: rfaB - UDP-glucose--(glucosyl)LPS alpha-1,2-glucosyltransferase, at 3,671,822 to 3,672,964
rfaB
Position (kb)
3670
3671
3672 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3669.819 kb on + strand, within waaO at 3669.820 kb on - strand, within waaO at 3669.824 kb on + strand, within waaO at 3669.925 kb on - strand, within waaO at 3670.067 kb on + strand at 3670.074 kb on + strand at 3670.384 kb on + strand, within rfaY at 3670.757 kb on - strand at 3670.984 kb on + strand, within rfaJ at 3671.010 kb on + strand, within rfaJ at 3671.179 kb on + strand, within rfaJ at 3671.310 kb on - strand, within rfaJ at 3671.513 kb on + strand, within rfaJ at 3671.593 kb on + strand, within rfaJ at 3671.598 kb on + strand, within rfaJ at 3671.637 kb on + strand, within rfaJ at 3671.904 kb on - strand at 3672.072 kb on - strand, within rfaB at 3672.198 kb on + strand, within rfaB at 3672.386 kb on - strand, within rfaB at 3672.601 kb on + strand, within rfaB at 3672.601 kb on + strand, within rfaB at 3672.601 kb on + strand, within rfaB at 3672.602 kb on - strand, within rfaB at 3672.607 kb on - strand, within rfaB at 3672.712 kb on + strand, within rfaB at 3672.779 kb on - strand, within rfaB at 3672.806 kb on - strand, within rfaB
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas31 remove 3,669,819 + waaO JDDGAC_19290 0.78 -1.1 3,669,820 - waaO JDDGAC_19290 0.78 -2.9 3,669,824 + waaO JDDGAC_19290 0.78 -2.1 3,669,925 - waaO JDDGAC_19290 0.88 -1.7 3,670,067 + +1.4 3,670,074 + +0.1 3,670,384 + rfaY JDDGAC_19295 0.45 +0.7 3,670,757 - -1.9 3,670,984 + rfaJ JDDGAC_19300 0.18 -2.5 3,671,010 + rfaJ JDDGAC_19300 0.21 -2.3 3,671,179 + rfaJ JDDGAC_19300 0.37 -1.7 3,671,310 - rfaJ JDDGAC_19300 0.50 -2.6 3,671,513 + rfaJ JDDGAC_19300 0.70 -3.0 3,671,593 + rfaJ JDDGAC_19300 0.78 -1.4 3,671,598 + rfaJ JDDGAC_19300 0.79 -1.6 3,671,637 + rfaJ JDDGAC_19300 0.83 -1.9 3,671,904 - -3.9 3,672,072 - rfaB JDDGAC_19305 0.22 -1.7 3,672,198 + rfaB JDDGAC_19305 0.33 -1.4 3,672,386 - rfaB JDDGAC_19305 0.49 +0.1 3,672,601 + rfaB JDDGAC_19305 0.68 -3.4 3,672,601 + rfaB JDDGAC_19305 0.68 -2.9 3,672,601 + rfaB JDDGAC_19305 0.68 -1.6 3,672,602 - rfaB JDDGAC_19305 0.68 -2.7 3,672,607 - rfaB JDDGAC_19305 0.69 -1.7 3,672,712 + rfaB JDDGAC_19305 0.78 -0.0 3,672,779 - rfaB JDDGAC_19305 0.84 -0.7 3,672,806 - rfaB JDDGAC_19305 0.86 -4.6
Or see this region's nucleotide sequence