Strain Fitness in Escherichia coli ECRC98 around JDDGAC_19185

Experiment: WM_Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntspoT and rpoZ are separated by 18 nucleotidesrpoZ and gmk are separated by 54 nucleotidesgmk and ligB are separated by 257 nucleotides JDDGAC_19180: spoT - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 3,649,639 to 3,651,747 spoT JDDGAC_19185: rpoZ - DNA-directed RNA polymerase subunit omega, at 3,651,766 to 3,652,041 rpoZ JDDGAC_19190: gmk - guanylate kinase, at 3,652,096 to 3,652,719 gmk JDDGAC_19195: ligB - NAD-dependent DNA ligase LigB, at 3,652,977 to 3,654,659 ligB Position (kb) 3651 3652 3653Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 3651.803 kb on - strand, within rpoZat 3652.046 kb on - strandat 3652.760 kb on - strandat 3652.760 kb on - strandat 3652.760 kb on - strandat 3652.865 kb on + strandat 3652.873 kb on + strandat 3652.943 kb on + strandat 3652.943 kb on + strandat 3652.943 kb on + strandat 3652.943 kb on + strandat 3652.943 kb on + strandat 3652.943 kb on + strandat 3652.943 kb on + strandat 3652.943 kb on + strandat 3652.943 kb on + strandat 3652.944 kb on - strandat 3652.944 kb on - strandat 3652.944 kb on - strandat 3652.944 kb on - strandat 3652.945 kb on + strandat 3652.946 kb on - strandat 3652.946 kb on - strandat 3652.984 kb on + strandat 3652.985 kb on - strandat 3652.985 kb on - strandat 3652.985 kb on - strandat 3653.000 kb on - strandat 3653.002 kb on + strandat 3653.002 kb on + strandat 3653.003 kb on - strandat 3653.003 kb on - strandat 3653.003 kb on - strandat 3653.003 kb on - strandat 3653.006 kb on + strandat 3653.007 kb on - strandat 3653.007 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas31
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3,651,803 - rpoZ JDDGAC_19185 0.13 -0.6
3,652,046 - -0.3
3,652,760 - +0.3
3,652,760 - -0.6
3,652,760 - +0.3
3,652,865 + +1.5
3,652,873 + -0.4
3,652,943 + +0.6
3,652,943 + +4.1
3,652,943 + -1.5
3,652,943 + -0.2
3,652,943 + -2.8
3,652,943 + -0.4
3,652,943 + -0.8
3,652,943 + +0.4
3,652,943 + +0.2
3,652,944 - -0.0
3,652,944 - -0.2
3,652,944 - -2.9
3,652,944 - -0.5
3,652,945 + +0.7
3,652,946 - -1.5
3,652,946 - -0.1
3,652,984 + -1.1
3,652,985 - +0.6
3,652,985 - +0.9
3,652,985 - -0.2
3,653,000 - +1.7
3,653,002 + -2.7
3,653,002 + +0.4
3,653,003 - -0.8
3,653,003 - -0.2
3,653,003 - +0.2
3,653,003 - +0.7
3,653,006 + -1.1
3,653,007 - +1.4
3,653,007 - -0.8

Or see this region's nucleotide sequence