Experiment: WM_Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt spoT and rpoZ are separated by 18 nucleotides rpoZ and gmk are separated by 54 nucleotides gmk and ligB are separated by 257 nucleotides
JDDGAC_19180: spoT - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 3,649,639 to 3,651,747
spoT
JDDGAC_19185: rpoZ - DNA-directed RNA polymerase subunit omega, at 3,651,766 to 3,652,041
rpoZ
JDDGAC_19190: gmk - guanylate kinase, at 3,652,096 to 3,652,719
gmk
JDDGAC_19195: ligB - NAD-dependent DNA ligase LigB, at 3,652,977 to 3,654,659
ligB
Position (kb)
3651
3652
3653 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3651.803 kb on - strand, within rpoZ at 3652.046 kb on - strand at 3652.760 kb on - strand at 3652.760 kb on - strand at 3652.760 kb on - strand at 3652.865 kb on + strand at 3652.873 kb on + strand at 3652.943 kb on + strand at 3652.943 kb on + strand at 3652.943 kb on + strand at 3652.943 kb on + strand at 3652.943 kb on + strand at 3652.943 kb on + strand at 3652.943 kb on + strand at 3652.943 kb on + strand at 3652.943 kb on + strand at 3652.944 kb on - strand at 3652.944 kb on - strand at 3652.944 kb on - strand at 3652.944 kb on - strand at 3652.945 kb on + strand at 3652.946 kb on - strand at 3652.946 kb on - strand at 3652.984 kb on + strand at 3652.985 kb on - strand at 3652.985 kb on - strand at 3652.985 kb on - strand at 3653.000 kb on - strand at 3653.002 kb on + strand at 3653.002 kb on + strand at 3653.003 kb on - strand at 3653.003 kb on - strand at 3653.003 kb on - strand at 3653.003 kb on - strand at 3653.006 kb on + strand at 3653.007 kb on - strand at 3653.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas31 remove 3,651,803 - rpoZ JDDGAC_19185 0.13 -0.6 3,652,046 - -0.3 3,652,760 - +0.3 3,652,760 - -0.6 3,652,760 - +0.3 3,652,865 + +1.5 3,652,873 + -0.4 3,652,943 + +0.6 3,652,943 + +4.1 3,652,943 + -1.5 3,652,943 + -0.2 3,652,943 + -2.8 3,652,943 + -0.4 3,652,943 + -0.8 3,652,943 + +0.4 3,652,943 + +0.2 3,652,944 - -0.0 3,652,944 - -0.2 3,652,944 - -2.9 3,652,944 - -0.5 3,652,945 + +0.7 3,652,946 - -1.5 3,652,946 - -0.1 3,652,984 + -1.1 3,652,985 - +0.6 3,652,985 - +0.9 3,652,985 - -0.2 3,653,000 - +1.7 3,653,002 + -2.7 3,653,002 + +0.4 3,653,003 - -0.8 3,653,003 - -0.2 3,653,003 - +0.2 3,653,003 - +0.7 3,653,006 + -1.1 3,653,007 - +1.4 3,653,007 - -0.8
Or see this region's nucleotide sequence