Strain Fitness in Escherichia coli ECRC98 around JDDGAC_14700

Experiment: WM_Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrC and thrB are separated by 0 nucleotidesthrB and thrA are separated by 1 nucleotides JDDGAC_14695: thrC - threonine synthase, at 2,740,948 to 2,742,234 thrC JDDGAC_14700: thrB - homoserine kinase, at 2,742,235 to 2,743,167 thrB JDDGAC_14705: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 2,743,169 to 2,745,631 thrA Position (kb) 2742 2743 2744Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2741.252 kb on + strand, within thrCat 2741.252 kb on + strand, within thrCat 2741.427 kb on + strand, within thrCat 2741.559 kb on - strand, within thrCat 2741.862 kb on + strand, within thrCat 2741.916 kb on + strand, within thrCat 2741.917 kb on - strand, within thrCat 2742.217 kb on - strandat 2742.225 kb on - strandat 2742.279 kb on + strandat 2742.279 kb on + strandat 2742.280 kb on - strandat 2742.308 kb on + strandat 2742.309 kb on - strandat 2742.394 kb on - strand, within thrBat 2742.480 kb on + strand, within thrBat 2742.481 kb on - strand, within thrBat 2742.627 kb on + strand, within thrBat 2742.749 kb on + strand, within thrBat 2742.749 kb on + strand, within thrBat 2742.750 kb on - strand, within thrBat 2742.881 kb on - strand, within thrBat 2742.900 kb on + strand, within thrBat 2742.972 kb on - strand, within thrBat 2742.978 kb on + strand, within thrBat 2743.063 kb on + strand, within thrBat 2743.063 kb on + strand, within thrBat 2743.063 kb on + strand, within thrBat 2743.178 kb on + strandat 2743.179 kb on - strandat 2743.179 kb on - strandat 2743.195 kb on + strandat 2743.199 kb on + strandat 2743.200 kb on - strandat 2743.200 kb on - strandat 2743.200 kb on - strandat 2743.278 kb on + strandat 2743.281 kb on + strandat 2743.282 kb on - strandat 2743.290 kb on + strandat 2743.290 kb on + strandat 2743.339 kb on + strandat 2743.340 kb on - strandat 2743.388 kb on - strandat 2743.398 kb on + strandat 2743.399 kb on - strandat 2743.580 kb on + strand, within thrAat 2743.583 kb on + strand, within thrAat 2743.584 kb on - strand, within thrAat 2743.596 kb on - strand, within thrAat 2743.603 kb on + strand, within thrAat 2743.604 kb on - strand, within thrAat 2743.691 kb on + strand, within thrAat 2743.692 kb on - strand, within thrAat 2743.707 kb on + strand, within thrAat 2743.708 kb on - strand, within thrAat 2743.870 kb on - strand, within thrAat 2743.942 kb on - strand, within thrAat 2744.002 kb on - strand, within thrAat 2744.022 kb on - strand, within thrAat 2744.084 kb on + strand, within thrAat 2744.119 kb on + strand, within thrAat 2744.138 kb on + strand, within thrAat 2744.139 kb on - strand, within thrAat 2744.147 kb on - strand, within thrA

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas31
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2,741,252 + thrC JDDGAC_14695 0.24 -1.9
2,741,252 + thrC JDDGAC_14695 0.24 -1.9
2,741,427 + thrC JDDGAC_14695 0.37 -0.6
2,741,559 - thrC JDDGAC_14695 0.47 -2.5
2,741,862 + thrC JDDGAC_14695 0.71 -0.5
2,741,916 + thrC JDDGAC_14695 0.75 -0.9
2,741,917 - thrC JDDGAC_14695 0.75 +1.9
2,742,217 - -1.8
2,742,225 - -0.7
2,742,279 + -0.2
2,742,279 + -0.9
2,742,280 - -1.5
2,742,308 + -0.6
2,742,309 - -0.5
2,742,394 - thrB JDDGAC_14700 0.17 -1.4
2,742,480 + thrB JDDGAC_14700 0.26 -0.7
2,742,481 - thrB JDDGAC_14700 0.26 -1.4
2,742,627 + thrB JDDGAC_14700 0.42 -0.9
2,742,749 + thrB JDDGAC_14700 0.55 -1.9
2,742,749 + thrB JDDGAC_14700 0.55 -0.1
2,742,750 - thrB JDDGAC_14700 0.55 -1.9
2,742,881 - thrB JDDGAC_14700 0.69 +0.1
2,742,900 + thrB JDDGAC_14700 0.71 -0.3
2,742,972 - thrB JDDGAC_14700 0.79 -0.9
2,742,978 + thrB JDDGAC_14700 0.80 -0.8
2,743,063 + thrB JDDGAC_14700 0.89 -0.1
2,743,063 + thrB JDDGAC_14700 0.89 -1.3
2,743,063 + thrB JDDGAC_14700 0.89 -2.4
2,743,178 + -1.9
2,743,179 - +0.1
2,743,179 - -0.7
2,743,195 + -2.2
2,743,199 + -2.6
2,743,200 - -0.8
2,743,200 - +0.2
2,743,200 - -0.5
2,743,278 + -1.8
2,743,281 + +0.3
2,743,282 - +1.0
2,743,290 + -0.9
2,743,290 + -1.1
2,743,339 + -3.0
2,743,340 - -0.5
2,743,388 - -2.1
2,743,398 + -1.5
2,743,399 - -0.6
2,743,580 + thrA JDDGAC_14705 0.17 +0.5
2,743,583 + thrA JDDGAC_14705 0.17 -1.9
2,743,584 - thrA JDDGAC_14705 0.17 -2.2
2,743,596 - thrA JDDGAC_14705 0.17 -2.8
2,743,603 + thrA JDDGAC_14705 0.18 -0.5
2,743,604 - thrA JDDGAC_14705 0.18 +1.1
2,743,691 + thrA JDDGAC_14705 0.21 -1.2
2,743,692 - thrA JDDGAC_14705 0.21 -1.4
2,743,707 + thrA JDDGAC_14705 0.22 -1.0
2,743,708 - thrA JDDGAC_14705 0.22 -1.9
2,743,870 - thrA JDDGAC_14705 0.28 -1.4
2,743,942 - thrA JDDGAC_14705 0.31 -1.7
2,744,002 - thrA JDDGAC_14705 0.34 -1.1
2,744,022 - thrA JDDGAC_14705 0.35 -0.9
2,744,084 + thrA JDDGAC_14705 0.37 -1.5
2,744,119 + thrA JDDGAC_14705 0.39 -2.3
2,744,138 + thrA JDDGAC_14705 0.39 -1.2
2,744,139 - thrA JDDGAC_14705 0.39 -2.6
2,744,147 - thrA JDDGAC_14705 0.40 +0.6

Or see this region's nucleotide sequence