Strain Fitness in Escherichia coli ECRC98 around JDDGAC_10475

Experiment: WM_Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttolA and tolR are separated by 64 nucleotidestolR and tolQ are separated by 3 nucleotidestolQ and ybgC overlap by 4 nucleotidesybgC and ybgE are separated by 149 nucleotidesybgE and cydX overlap by 1 nucleotidescydX and cydB are separated by 14 nucleotides JDDGAC_10465: tolA - cell envelope integrity protein TolA, at 1,863,282 to 1,864,496 tolA JDDGAC_10470: tolR - colicin uptake protein TolR, at 1,864,561 to 1,864,989 tolR JDDGAC_10475: tolQ - Tol-Pal system protein TolQ, at 1,864,993 to 1,865,685 tolQ JDDGAC_10480: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 1,865,682 to 1,866,086 ybgC JDDGAC_10485: ybgE - cyd operon protein YbgE, at 1,866,236 to 1,866,529 ybgE JDDGAC_10490: cydX - cytochrome bd-I oxidase subunit CydX, at 1,866,529 to 1,866,642 cydX JDDGAC_10495: cydB - cytochrome d ubiquinol oxidase subunit II, at 1,866,657 to 1,867,796 cydB Position (kb) 1864 1865 1866Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1864.422 kb on + strandat 1864.449 kb on - strandat 1864.453 kb on - strandat 1864.562 kb on + strandat 1864.563 kb on - strandat 1864.566 kb on + strandat 1864.567 kb on - strandat 1864.567 kb on - strandat 1864.609 kb on + strand, within tolRat 1864.609 kb on + strand, within tolRat 1864.626 kb on + strand, within tolRat 1864.770 kb on - strand, within tolRat 1864.918 kb on + strand, within tolRat 1864.933 kb on + strand, within tolRat 1864.934 kb on - strand, within tolRat 1865.111 kb on + strand, within tolQat 1865.270 kb on + strand, within tolQat 1865.271 kb on - strand, within tolQat 1865.271 kb on - strand, within tolQat 1865.346 kb on + strand, within tolQat 1865.802 kb on - strand, within ybgCat 1865.842 kb on - strand, within ybgCat 1865.854 kb on - strand, within ybgCat 1865.907 kb on - strand, within ybgCat 1865.907 kb on - strand, within ybgCat 1866.004 kb on + strand, within ybgCat 1866.005 kb on - strand, within ybgCat 1866.046 kb on + strandat 1866.046 kb on + strandat 1866.047 kb on - strandat 1866.047 kb on - strandat 1866.049 kb on + strandat 1866.080 kb on + strandat 1866.081 kb on - strandat 1866.090 kb on - strandat 1866.114 kb on + strandat 1866.133 kb on + strandat 1866.137 kb on - strandat 1866.138 kb on + strandat 1866.436 kb on + strand, within ybgEat 1866.505 kb on - strandat 1866.505 kb on - strandat 1866.523 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas31
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1,864,422 + -1.4
1,864,449 - -2.2
1,864,453 - -0.0
1,864,562 + -0.8
1,864,563 - +0.2
1,864,566 + -1.5
1,864,567 - +0.0
1,864,567 - +0.2
1,864,609 + tolR JDDGAC_10470 0.11 +1.8
1,864,609 + tolR JDDGAC_10470 0.11 -0.3
1,864,626 + tolR JDDGAC_10470 0.15 -0.8
1,864,770 - tolR JDDGAC_10470 0.49 +0.1
1,864,918 + tolR JDDGAC_10470 0.83 -2.9
1,864,933 + tolR JDDGAC_10470 0.87 -0.5
1,864,934 - tolR JDDGAC_10470 0.87 -2.5
1,865,111 + tolQ JDDGAC_10475 0.17 -3.1
1,865,270 + tolQ JDDGAC_10475 0.40 -2.5
1,865,271 - tolQ JDDGAC_10475 0.40 -1.4
1,865,271 - tolQ JDDGAC_10475 0.40 -1.5
1,865,346 + tolQ JDDGAC_10475 0.51 -0.5
1,865,802 - ybgC JDDGAC_10480 0.30 +0.1
1,865,842 - ybgC JDDGAC_10480 0.40 -0.1
1,865,854 - ybgC JDDGAC_10480 0.42 +0.1
1,865,907 - ybgC JDDGAC_10480 0.56 +0.6
1,865,907 - ybgC JDDGAC_10480 0.56 -0.1
1,866,004 + ybgC JDDGAC_10480 0.80 -0.4
1,866,005 - ybgC JDDGAC_10480 0.80 -0.0
1,866,046 + -2.6
1,866,046 + -1.3
1,866,047 - +0.4
1,866,047 - +0.8
1,866,049 + -1.1
1,866,080 + -0.0
1,866,081 - -1.1
1,866,090 - +0.3
1,866,114 + -0.3
1,866,133 + +0.3
1,866,137 - -2.8
1,866,138 + +1.1
1,866,436 + ybgE JDDGAC_10485 0.68 -1.3
1,866,505 - +0.0
1,866,505 - -0.1
1,866,523 + +0.2

Or see this region's nucleotide sequence