Experiment: WM_Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cpoB and pal are separated by 9 nucleotides pal and tolB are separated by 34 nucleotides tolB and tolA are separated by 132 nucleotides
JDDGAC_10450: cpoB - cell division protein CpoB, at 1,860,500 to 1,861,291
cpoB
JDDGAC_10455: pal - peptidoglycan-associated lipoprotein Pal, at 1,861,301 to 1,861,822
pal
JDDGAC_10460: tolB - Tol-Pal system protein TolB, at 1,861,857 to 1,863,149
tolB
JDDGAC_10465: tolA - cell envelope integrity protein TolA, at 1,863,282 to 1,864,496
tolA
Position (kb) 1861
1862
1863
1864 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1861.145 kb on - strand, within cpoB at 1861.240 kb on + strand at 1861.240 kb on + strand at 1861.240 kb on + strand at 1861.250 kb on + strand at 1861.266 kb on - strand at 1861.282 kb on + strand at 1861.302 kb on + strand at 1861.306 kb on - strand at 1861.380 kb on + strand, within pal at 1861.534 kb on + strand, within pal at 1861.591 kb on - strand, within pal at 1861.591 kb on - strand, within pal at 1861.609 kb on - strand, within pal at 1861.935 kb on - strand at 1862.047 kb on - strand, within tolB at 1862.171 kb on + strand, within tolB at 1862.172 kb on - strand, within tolB at 1862.172 kb on - strand, within tolB at 1862.172 kb on - strand, within tolB at 1862.172 kb on - strand, within tolB at 1862.172 kb on - strand, within tolB at 1862.172 kb on - strand, within tolB at 1862.205 kb on - strand, within tolB at 1862.238 kb on - strand, within tolB at 1862.335 kb on - strand, within tolB at 1862.335 kb on - strand, within tolB at 1862.339 kb on - strand, within tolB at 1862.339 kb on - strand, within tolB at 1862.443 kb on - strand, within tolB at 1862.503 kb on + strand, within tolB at 1862.504 kb on - strand, within tolB at 1862.598 kb on + strand, within tolB at 1862.644 kb on + strand, within tolB at 1862.645 kb on - strand, within tolB at 1862.672 kb on + strand, within tolB at 1862.673 kb on - strand, within tolB at 1862.767 kb on - strand, within tolB at 1862.815 kb on + strand, within tolB at 1862.816 kb on - strand, within tolB at 1862.853 kb on + strand, within tolB at 1862.893 kb on - strand, within tolB at 1862.960 kb on + strand, within tolB at 1863.058 kb on - strand at 1863.112 kb on - strand at 1863.152 kb on + strand at 1863.153 kb on - strand at 1863.162 kb on - strand at 1863.322 kb on + strand at 1863.490 kb on + strand, within tolA at 1863.490 kb on + strand, within tolA at 1863.533 kb on + strand, within tolA at 1863.534 kb on - strand, within tolA at 1863.534 kb on - strand, within tolA at 1863.635 kb on + strand, within tolA at 1863.660 kb on - strand, within tolA
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas31 remove 1,861,145 - cpoB JDDGAC_10450 0.81 -0.2 1,861,240 + -1.5 1,861,240 + -0.3 1,861,240 + -0.2 1,861,250 + -0.5 1,861,266 - +0.1 1,861,282 + +0.9 1,861,302 + -0.4 1,861,306 - -0.3 1,861,380 + pal JDDGAC_10455 0.15 -0.7 1,861,534 + pal JDDGAC_10455 0.45 -1.9 1,861,591 - pal JDDGAC_10455 0.56 -0.8 1,861,591 - pal JDDGAC_10455 0.56 -1.1 1,861,609 - pal JDDGAC_10455 0.59 -0.9 1,861,935 - +0.3 1,862,047 - tolB JDDGAC_10460 0.15 -0.3 1,862,171 + tolB JDDGAC_10460 0.24 +3.1 1,862,172 - tolB JDDGAC_10460 0.24 -2.6 1,862,172 - tolB JDDGAC_10460 0.24 -1.4 1,862,172 - tolB JDDGAC_10460 0.24 -1.9 1,862,172 - tolB JDDGAC_10460 0.24 -1.7 1,862,172 - tolB JDDGAC_10460 0.24 +2.1 1,862,172 - tolB JDDGAC_10460 0.24 -0.9 1,862,205 - tolB JDDGAC_10460 0.27 -0.1 1,862,238 - tolB JDDGAC_10460 0.29 -3.1 1,862,335 - tolB JDDGAC_10460 0.37 -1.3 1,862,335 - tolB JDDGAC_10460 0.37 -0.5 1,862,339 - tolB JDDGAC_10460 0.37 -3.1 1,862,339 - tolB JDDGAC_10460 0.37 +0.6 1,862,443 - tolB JDDGAC_10460 0.45 +0.8 1,862,503 + tolB JDDGAC_10460 0.50 -2.8 1,862,504 - tolB JDDGAC_10460 0.50 -3.2 1,862,598 + tolB JDDGAC_10460 0.57 +0.1 1,862,644 + tolB JDDGAC_10460 0.61 -1.6 1,862,645 - tolB JDDGAC_10460 0.61 -2.8 1,862,672 + tolB JDDGAC_10460 0.63 -1.6 1,862,673 - tolB JDDGAC_10460 0.63 -0.7 1,862,767 - tolB JDDGAC_10460 0.70 -1.0 1,862,815 + tolB JDDGAC_10460 0.74 -1.3 1,862,816 - tolB JDDGAC_10460 0.74 -1.0 1,862,853 + tolB JDDGAC_10460 0.77 -1.6 1,862,893 - tolB JDDGAC_10460 0.80 +2.1 1,862,960 + tolB JDDGAC_10460 0.85 -2.0 1,863,058 - -3.1 1,863,112 - -3.1 1,863,152 + -3.3 1,863,153 - +1.3 1,863,162 - -0.2 1,863,322 + -1.3 1,863,490 + tolA JDDGAC_10465 0.17 +1.3 1,863,490 + tolA JDDGAC_10465 0.17 -1.4 1,863,533 + tolA JDDGAC_10465 0.21 -1.1 1,863,534 - tolA JDDGAC_10465 0.21 -2.1 1,863,534 - tolA JDDGAC_10465 0.21 -1.4 1,863,635 + tolA JDDGAC_10465 0.29 -0.5 1,863,660 - tolA JDDGAC_10465 0.31 -0.9
Or see this region's nucleotide sequence