Strain Fitness in Escherichia coli ECRC98 around JDDGAC_06140

Experiment: WM_Bas31

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthemA and lolB are separated by 213 nucleotideslolB and ispE overlap by 1 nucleotidesispE and prs are separated by 84 nucleotides JDDGAC_06130: hemA - glutamyl-tRNA reductase, at 1,066,696 to 1,067,952 hemA JDDGAC_06135: lolB - lipoprotein insertase outer membrane protein LolB, at 1,068,166 to 1,068,789 lolB JDDGAC_06140: ispE - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 1,068,789 to 1,069,640 ispE JDDGAC_06145: prs - ribose-phosphate diphosphokinase, at 1,069,725 to 1,070,738 prs Position (kb) 1068 1069 1070Strain fitness (log2 ratio) -3 -2 -1 0 1at 1068.123 kb on + strandat 1068.132 kb on + strandat 1068.343 kb on + strand, within lolBat 1068.650 kb on - strand, within lolBat 1069.296 kb on + strand, within ispEat 1069.297 kb on - strand, within ispEat 1070.227 kb on + strand, within prsat 1070.521 kb on + strand, within prsat 1070.577 kb on - strand, within prs

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas31
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1,068,123 + +0.1
1,068,132 + +0.3
1,068,343 + lolB JDDGAC_06135 0.28 +0.9
1,068,650 - lolB JDDGAC_06135 0.78 +1.0
1,069,296 + ispE JDDGAC_06140 0.60 -0.4
1,069,297 - ispE JDDGAC_06140 0.60 +0.1
1,070,227 + prs JDDGAC_06145 0.50 -0.9
1,070,521 + prs JDDGAC_06145 0.79 -3.1
1,070,577 - prs JDDGAC_06145 0.84 -0.2

Or see this region's nucleotide sequence