Experiment: WM_Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynjI and gdhA are separated by 116 nucleotides gdhA and ynjH are separated by 235 nucleotides ynjH and nudG overlap by 35 nucleotides nudG and ynjF are separated by 86 nucleotides
JDDGAC_01410: ynjI - Inner membrane protein YnjI, at 244,856 to 245,896
ynjI
JDDGAC_01415: gdhA - NADP-specific glutamate dehydrogenase, at 246,013 to 247,356
gdhA
JDDGAC_01420: ynjH - Uncharacterized protein YnjH, at 247,592 to 247,864
ynjH
JDDGAC_01425: nudG - CTP pyrophosphohydrolase, at 247,830 to 248,237
nudG
JDDGAC_01430: ynjF - Inner membrane protein YnjF, at 248,324 to 248,944
ynjF
Position (kb) 246
247
248 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 245.232 kb on - strand, within ynjI at 245.304 kb on - strand, within ynjI at 245.337 kb on + strand, within ynjI at 245.383 kb on + strand, within ynjI at 245.659 kb on + strand, within ynjI at 245.660 kb on - strand, within ynjI at 245.662 kb on - strand, within ynjI at 245.749 kb on + strand, within ynjI at 245.749 kb on + strand, within ynjI at 245.829 kb on - strand at 245.829 kb on - strand at 246.008 kb on - strand at 246.083 kb on + strand at 246.084 kb on - strand at 246.240 kb on + strand, within gdhA at 246.241 kb on - strand, within gdhA at 246.313 kb on - strand, within gdhA at 246.313 kb on - strand, within gdhA at 246.313 kb on - strand, within gdhA at 246.554 kb on - strand, within gdhA at 246.689 kb on - strand, within gdhA at 246.742 kb on - strand, within gdhA at 246.997 kb on - strand, within gdhA at 247.264 kb on + strand at 247.265 kb on - strand at 247.363 kb on - strand at 247.373 kb on + strand at 247.373 kb on + strand at 247.375 kb on + strand at 247.376 kb on - strand at 247.403 kb on - strand at 247.403 kb on - strand at 247.430 kb on + strand at 247.432 kb on + strand at 247.433 kb on - strand at 247.453 kb on - strand at 247.486 kb on - strand at 247.562 kb on - strand at 247.633 kb on - strand, within ynjH at 247.674 kb on + strand, within ynjH at 247.674 kb on + strand, within ynjH at 247.675 kb on - strand, within ynjH at 247.778 kb on - strand, within ynjH at 247.791 kb on + strand, within ynjH at 247.831 kb on + strand at 247.831 kb on + strand at 248.042 kb on - strand, within nudG at 248.042 kb on - strand, within nudG at 248.127 kb on - strand, within nudG at 248.275 kb on + strand at 248.306 kb on + strand at 248.328 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas31 remove 245,232 - ynjI JDDGAC_01410 0.36 +0.3 245,304 - ynjI JDDGAC_01410 0.43 -1.2 245,337 + ynjI JDDGAC_01410 0.46 -0.3 245,383 + ynjI JDDGAC_01410 0.51 +0.8 245,659 + ynjI JDDGAC_01410 0.77 +1.5 245,660 - ynjI JDDGAC_01410 0.77 -0.4 245,662 - ynjI JDDGAC_01410 0.77 +1.6 245,749 + ynjI JDDGAC_01410 0.86 -0.8 245,749 + ynjI JDDGAC_01410 0.86 -0.3 245,829 - -0.4 245,829 - -0.3 246,008 - -1.7 246,083 + +0.9 246,084 - +1.4 246,240 + gdhA JDDGAC_01415 0.17 -0.4 246,241 - gdhA JDDGAC_01415 0.17 +0.4 246,313 - gdhA JDDGAC_01415 0.22 -0.9 246,313 - gdhA JDDGAC_01415 0.22 -1.5 246,313 - gdhA JDDGAC_01415 0.22 -0.7 246,554 - gdhA JDDGAC_01415 0.40 -0.1 246,689 - gdhA JDDGAC_01415 0.50 -2.7 246,742 - gdhA JDDGAC_01415 0.54 -2.8 246,997 - gdhA JDDGAC_01415 0.73 -1.0 247,264 + -1.3 247,265 - +0.1 247,363 - -0.9 247,373 + +1.1 247,373 + -0.3 247,375 + -1.7 247,376 - +0.2 247,403 - +0.5 247,403 - -0.4 247,430 + -1.6 247,432 + +0.3 247,433 - +0.5 247,453 - -0.4 247,486 - -1.3 247,562 - -1.1 247,633 - ynjH JDDGAC_01420 0.15 -0.8 247,674 + ynjH JDDGAC_01420 0.30 +2.1 247,674 + ynjH JDDGAC_01420 0.30 -1.1 247,675 - ynjH JDDGAC_01420 0.30 -2.0 247,778 - ynjH JDDGAC_01420 0.68 +0.2 247,791 + ynjH JDDGAC_01420 0.73 +0.4 247,831 + -2.3 247,831 + -1.3 248,042 - nudG JDDGAC_01425 0.52 -0.1 248,042 - nudG JDDGAC_01425 0.52 +1.4 248,127 - nudG JDDGAC_01425 0.73 +1.0 248,275 + +0.4 248,306 + +0.1 248,328 + +0.5
Or see this region's nucleotide sequence