Experiment: WM_Bas31
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyk and hexR are separated by 127 nucleotides hexR and JDDGAC_00905 are separated by 22 nucleotides JDDGAC_00905 and zwf are separated by 165 nucleotides
JDDGAC_00895: pyk - pyruvate kinase, at 148,317 to 149,759
pyk
JDDGAC_00900: hexR - DNA-binding transcriptional regulator HexR, at 149,887 to 150,756
hexR
JDDGAC_00905: JDDGAC_00905 - hypothetical protein, at 150,779 to 150,928
_00905
JDDGAC_00910: zwf - glucose-6-phosphate dehydrogenase, at 151,094 to 152,569
zwf
Position (kb) 149
150
151 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 149.214 kb on - strand, within pyk at 149.420 kb on - strand, within pyk at 149.527 kb on - strand, within pyk at 149.652 kb on - strand at 149.761 kb on + strand at 149.762 kb on - strand at 149.762 kb on - strand at 149.840 kb on + strand at 149.841 kb on - strand at 149.861 kb on + strand at 149.908 kb on - strand at 149.912 kb on - strand at 149.965 kb on - strand at 149.995 kb on - strand, within hexR at 150.016 kb on + strand, within hexR at 150.057 kb on - strand, within hexR at 150.057 kb on - strand, within hexR at 150.076 kb on + strand, within hexR at 150.246 kb on + strand, within hexR at 150.247 kb on - strand, within hexR at 150.247 kb on - strand, within hexR at 150.312 kb on + strand, within hexR at 150.313 kb on - strand, within hexR at 150.463 kb on + strand, within hexR at 150.519 kb on - strand, within hexR at 150.649 kb on - strand, within hexR at 150.761 kb on + strand at 150.778 kb on - strand at 150.825 kb on + strand, within JDDGAC_00905 at 150.826 kb on - strand, within JDDGAC_00905 at 150.829 kb on - strand, within JDDGAC_00905 at 150.829 kb on - strand, within JDDGAC_00905 at 150.964 kb on + strand at 151.034 kb on - strand at 151.042 kb on + strand at 151.060 kb on + strand at 151.076 kb on + strand at 151.076 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas31 remove 149,214 - pyk JDDGAC_00895 0.62 +0.1 149,420 - pyk JDDGAC_00895 0.76 +0.2 149,527 - pyk JDDGAC_00895 0.84 +0.5 149,652 - -1.2 149,761 + -1.3 149,762 - +0.3 149,762 - -0.0 149,840 + +1.2 149,841 - -2.6 149,861 + +0.2 149,908 - +1.1 149,912 - +0.7 149,965 - -0.2 149,995 - hexR JDDGAC_00900 0.12 +0.5 150,016 + hexR JDDGAC_00900 0.15 +0.1 150,057 - hexR JDDGAC_00900 0.20 -0.5 150,057 - hexR JDDGAC_00900 0.20 -2.1 150,076 + hexR JDDGAC_00900 0.22 +0.2 150,246 + hexR JDDGAC_00900 0.41 -0.4 150,247 - hexR JDDGAC_00900 0.41 -1.4 150,247 - hexR JDDGAC_00900 0.41 +0.3 150,312 + hexR JDDGAC_00900 0.49 +0.4 150,313 - hexR JDDGAC_00900 0.49 -1.4 150,463 + hexR JDDGAC_00900 0.66 +0.3 150,519 - hexR JDDGAC_00900 0.73 -0.9 150,649 - hexR JDDGAC_00900 0.88 +0.1 150,761 + -0.7 150,778 - +0.4 150,825 + JDDGAC_00905 0.31 +0.4 150,826 - JDDGAC_00905 0.31 -1.0 150,829 - JDDGAC_00905 0.33 -0.6 150,829 - JDDGAC_00905 0.33 -0.2 150,964 + -0.1 151,034 - -4.0 151,042 + -0.4 151,060 + -0.1 151,076 + -0.2 151,076 + +0.8
Or see this region's nucleotide sequence