Strain Fitness in Escherichia coli ECRC98 around JDDGAC_25290

Experiment: WM_JK45

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrmJ and iscR are separated by 300 nucleotidesiscR and iscS are separated by 111 nucleotides JDDGAC_25285: trmJ - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 4,836,916 to 4,837,653 trmJ JDDGAC_25290: iscR - Fe-S cluster assembly transcriptional regulator IscR, at 4,837,954 to 4,838,442 iscR JDDGAC_25295: iscS - cysteine desulfurase, at 4,838,554 to 4,839,768 iscS Position (kb) 4837 4838 4839Strain fitness (log2 ratio) -3 -2 -1 0 1at 4836.967 kb on - strandat 4837.089 kb on + strand, within trmJat 4837.090 kb on - strand, within trmJat 4837.135 kb on - strand, within trmJat 4837.233 kb on + strand, within trmJat 4837.303 kb on + strand, within trmJat 4837.303 kb on + strand, within trmJat 4837.386 kb on + strand, within trmJat 4837.620 kb on + strandat 4837.622 kb on + strandat 4837.622 kb on + strandat 4837.641 kb on + strandat 4837.642 kb on - strandat 4837.642 kb on - strandat 4837.709 kb on - strandat 4837.895 kb on + strandat 4837.907 kb on + strandat 4837.948 kb on + strandat 4837.948 kb on + strandat 4837.968 kb on + strandat 4837.968 kb on + strandat 4837.968 kb on + strandat 4837.968 kb on + strandat 4838.126 kb on + strand, within iscRat 4838.146 kb on + strand, within iscRat 4838.213 kb on + strand, within iscRat 4838.325 kb on + strand, within iscRat 4838.325 kb on + strand, within iscRat 4838.325 kb on + strand, within iscRat 4838.325 kb on + strand, within iscRat 4838.325 kb on + strand, within iscRat 4838.440 kb on + strandat 4838.440 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_JK45
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4,836,967 - +0.9
4,837,089 + trmJ JDDGAC_25285 0.23 -0.0
4,837,090 - trmJ JDDGAC_25285 0.24 +0.3
4,837,135 - trmJ JDDGAC_25285 0.30 -0.7
4,837,233 + trmJ JDDGAC_25285 0.43 -0.3
4,837,303 + trmJ JDDGAC_25285 0.52 +0.2
4,837,303 + trmJ JDDGAC_25285 0.52 -0.7
4,837,386 + trmJ JDDGAC_25285 0.64 +0.2
4,837,620 + +0.2
4,837,622 + -2.2
4,837,622 + +0.6
4,837,641 + -1.1
4,837,642 - +0.0
4,837,642 - +0.9
4,837,709 - +0.9
4,837,895 + -0.7
4,837,907 + +0.1
4,837,948 + -0.3
4,837,948 + -0.0
4,837,968 + -1.8
4,837,968 + -1.0
4,837,968 + -0.6
4,837,968 + -1.2
4,838,126 + iscR JDDGAC_25290 0.35 +0.1
4,838,146 + iscR JDDGAC_25290 0.39 -1.5
4,838,213 + iscR JDDGAC_25290 0.53 -0.8
4,838,325 + iscR JDDGAC_25290 0.76 -0.6
4,838,325 + iscR JDDGAC_25290 0.76 -0.8
4,838,325 + iscR JDDGAC_25290 0.76 -3.1
4,838,325 + iscR JDDGAC_25290 0.76 -1.3
4,838,325 + iscR JDDGAC_25290 0.76 -0.0
4,838,440 + +0.4
4,838,440 + +0.0

Or see this region's nucleotide sequence