Experiment: WM_JK45
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Gly_trna and Gly_trna are separated by 36 nucleotides Gly_trna and orn are separated by 210 nucleotides orn and rsgA are separated by 94 nucleotides rsgA and asd are separated by 96 nucleotides
JDDGAC_15940: Gly_trna - tRNA-Gly(gcc), at 2,999,600 to 2,999,675
_trna
JDDGAC_15945: Gly_trna - tRNA-Gly(gcc), at 2,999,712 to 2,999,787
_trna
JDDGAC_15950: orn - oligoribonuclease, at 2,999,998 to 3,000,543
orn
JDDGAC_15955: rsgA - small ribosomal subunit biogenesis GTPase RsgA, at 3,000,638 to 3,001,690
rsgA
JDDGAC_15960: asd - archaetidylserine decarboxylase, at 3,001,787 to 3,002,755
asd
Position (kb)
3000
3001
3002 Strain fitness (log2 ratio)
-1
0
1 at 2999.790 kb on - strand at 2999.793 kb on - strand at 2999.793 kb on - strand at 2999.793 kb on - strand at 2999.979 kb on + strand at 3000.571 kb on - strand at 3000.571 kb on - strand at 3000.571 kb on - strand at 3000.573 kb on - strand at 3000.573 kb on - strand at 3000.593 kb on - strand at 3000.597 kb on + strand at 3000.597 kb on + strand at 3000.597 kb on + strand at 3001.654 kb on - strand at 3001.678 kb on + strand at 3001.695 kb on + strand at 3001.695 kb on + strand at 3001.695 kb on + strand at 3001.700 kb on + strand at 3001.710 kb on + strand at 3001.744 kb on + strand at 3002.640 kb on + strand, within asd at 3002.640 kb on + strand, within asd at 3002.659 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_JK45 remove 2,999,790 - -0.9 2,999,793 - -0.0 2,999,793 - -0.1 2,999,793 - -1.0 2,999,979 + -0.0 3,000,571 - +0.2 3,000,571 - +0.1 3,000,571 - -0.3 3,000,573 - +0.1 3,000,573 - -0.5 3,000,593 - +0.1 3,000,597 + +0.1 3,000,597 + +1.0 3,000,597 + -0.1 3,001,654 - -0.0 3,001,678 + -0.0 3,001,695 + -0.9 3,001,695 + +0.2 3,001,695 + +0.9 3,001,700 + -0.6 3,001,710 + -0.2 3,001,744 + +0.8 3,002,640 + asd JDDGAC_15960 0.88 -0.9 3,002,640 + asd JDDGAC_15960 0.88 -0.1 3,002,659 + +0.5
Or see this region's nucleotide sequence