Experiment: WM_JK45
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt icd and rluE are separated by 171 nucleotides rluE and nudJ are separated by 9 nucleotides nudJ and mnmA are separated by 53 nucleotides
JDDGAC_06605: icd - NADP-dependent isocitrate dehydrogenase, at 1,151,066 to 1,152,316
icd
JDDGAC_06610: rluE - 23S rRNA pseudouridine(2457) synthase RluE, at 1,152,488 to 1,153,141
rluE
JDDGAC_06615: nudJ - phosphatase NudJ, at 1,153,151 to 1,153,612
nudJ
JDDGAC_06620: mnmA - tRNA 2-thiouridine(34) synthase MnmA, at 1,153,666 to 1,154,772
mnmA
Position (kb) 1152
1153
1154 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1151.723 kb on - strand, within icd at 1152.368 kb on - strand at 1152.484 kb on + strand at 1152.536 kb on - strand at 1152.575 kb on - strand, within rluE at 1152.583 kb on + strand, within rluE at 1152.704 kb on + strand, within rluE at 1152.704 kb on + strand, within rluE at 1152.812 kb on + strand, within rluE at 1152.812 kb on + strand, within rluE at 1152.815 kb on + strand, within rluE at 1152.815 kb on + strand, within rluE at 1152.815 kb on + strand, within rluE at 1152.880 kb on - strand, within rluE at 1153.002 kb on + strand, within rluE at 1153.002 kb on + strand, within rluE at 1153.003 kb on - strand, within rluE at 1153.003 kb on - strand, within rluE at 1153.003 kb on - strand, within rluE at 1153.004 kb on + strand, within rluE at 1153.004 kb on + strand, within rluE at 1153.004 kb on + strand, within rluE at 1153.004 kb on + strand, within rluE at 1153.092 kb on - strand at 1153.139 kb on + strand at 1153.139 kb on + strand at 1153.351 kb on + strand, within nudJ at 1153.529 kb on - strand, within nudJ at 1154.123 kb on - strand, within mnmA
Per-strain Table
Position Strand Gene LocusTag Fraction WM_JK45 remove 1,151,723 - icd JDDGAC_06605 0.53 +2.2 1,152,368 - +1.2 1,152,484 + +1.1 1,152,536 - +0.5 1,152,575 - rluE JDDGAC_06610 0.13 -0.6 1,152,583 + rluE JDDGAC_06610 0.15 -0.2 1,152,704 + rluE JDDGAC_06610 0.33 +0.6 1,152,704 + rluE JDDGAC_06610 0.33 +0.1 1,152,812 + rluE JDDGAC_06610 0.50 -0.0 1,152,812 + rluE JDDGAC_06610 0.50 -0.0 1,152,815 + rluE JDDGAC_06610 0.50 -3.2 1,152,815 + rluE JDDGAC_06610 0.50 +0.4 1,152,815 + rluE JDDGAC_06610 0.50 +1.8 1,152,880 - rluE JDDGAC_06610 0.60 +2.0 1,153,002 + rluE JDDGAC_06610 0.79 +1.3 1,153,002 + rluE JDDGAC_06610 0.79 +0.9 1,153,003 - rluE JDDGAC_06610 0.79 +0.9 1,153,003 - rluE JDDGAC_06610 0.79 +0.8 1,153,003 - rluE JDDGAC_06610 0.79 +0.5 1,153,004 + rluE JDDGAC_06610 0.79 -1.8 1,153,004 + rluE JDDGAC_06610 0.79 -0.9 1,153,004 + rluE JDDGAC_06610 0.79 +0.4 1,153,004 + rluE JDDGAC_06610 0.79 +0.3 1,153,092 - +0.9 1,153,139 + +0.5 1,153,139 + -0.0 1,153,351 + nudJ JDDGAC_06615 0.43 -0.3 1,153,529 - nudJ JDDGAC_06615 0.82 +0.0 1,154,123 - mnmA JDDGAC_06620 0.41 +0.8
Or see this region's nucleotide sequence