Experiment: WM_JK45
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaK and wcaL overlap by 4 nucleotides wcaL and wcaM are separated by 10 nucleotides
JDDGAC_28495: wcaK - colanic acid biosynthesis pyruvyl transferase WcaK, at 5,465,452 to 5,466,732
wcaK
JDDGAC_28500: wcaL - colanic acid biosynthesis glycosyltransferase WcaL, at 5,466,729 to 5,467,949
wcaL
JDDGAC_28505: wcaM - colanic acid biosynthesis protein WcaM, at 5,467,960 to 5,469,354
wcaM
Position (kb)
5466
5467
5468 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5465.815 kb on - strand, within wcaK at 5465.815 kb on - strand, within wcaK at 5465.886 kb on + strand, within wcaK at 5466.115 kb on + strand, within wcaK at 5466.169 kb on - strand, within wcaK at 5466.223 kb on + strand, within wcaK at 5466.313 kb on + strand, within wcaK at 5466.314 kb on - strand, within wcaK at 5466.315 kb on + strand, within wcaK at 5466.316 kb on - strand, within wcaK at 5466.384 kb on - strand, within wcaK at 5466.511 kb on + strand, within wcaK at 5466.512 kb on - strand, within wcaK at 5466.519 kb on + strand, within wcaK at 5466.520 kb on - strand, within wcaK at 5466.652 kb on - strand at 5466.878 kb on + strand, within wcaL at 5466.879 kb on - strand, within wcaL at 5466.879 kb on - strand, within wcaL at 5467.219 kb on - strand, within wcaL at 5467.345 kb on + strand, within wcaL at 5467.346 kb on - strand, within wcaL at 5467.346 kb on - strand, within wcaL at 5467.349 kb on + strand, within wcaL at 5467.350 kb on - strand, within wcaL at 5467.551 kb on + strand, within wcaL at 5467.947 kb on + strand at 5467.959 kb on + strand at 5467.960 kb on - strand at 5468.025 kb on + strand at 5468.026 kb on - strand at 5468.067 kb on + strand at 5468.068 kb on - strand at 5468.077 kb on + strand at 5468.078 kb on - strand at 5468.078 kb on - strand at 5468.167 kb on - strand, within wcaM at 5468.205 kb on - strand, within wcaM at 5468.253 kb on - strand, within wcaM at 5468.292 kb on + strand, within wcaM at 5468.299 kb on - strand, within wcaM at 5468.299 kb on - strand, within wcaM at 5468.439 kb on + strand, within wcaM at 5468.449 kb on - strand, within wcaM at 5468.453 kb on - strand, within wcaM at 5468.463 kb on - strand, within wcaM at 5468.512 kb on - strand, within wcaM at 5468.610 kb on + strand, within wcaM at 5468.662 kb on + strand, within wcaM at 5468.663 kb on - strand, within wcaM at 5468.670 kb on + strand, within wcaM at 5468.670 kb on + strand, within wcaM at 5468.745 kb on - strand, within wcaM at 5468.796 kb on + strand, within wcaM at 5468.852 kb on - strand, within wcaM at 5468.878 kb on - strand, within wcaM at 5468.929 kb on - strand, within wcaM
Per-strain Table
Position Strand Gene LocusTag Fraction WM_JK45 remove 5,465,815 - wcaK JDDGAC_28495 0.28 +0.5 5,465,815 - wcaK JDDGAC_28495 0.28 -0.8 5,465,886 + wcaK JDDGAC_28495 0.34 -0.3 5,466,115 + wcaK JDDGAC_28495 0.52 -0.3 5,466,169 - wcaK JDDGAC_28495 0.56 +2.4 5,466,223 + wcaK JDDGAC_28495 0.60 -0.2 5,466,313 + wcaK JDDGAC_28495 0.67 -0.1 5,466,314 - wcaK JDDGAC_28495 0.67 +0.6 5,466,315 + wcaK JDDGAC_28495 0.67 +0.2 5,466,316 - wcaK JDDGAC_28495 0.67 +0.5 5,466,384 - wcaK JDDGAC_28495 0.73 +0.2 5,466,511 + wcaK JDDGAC_28495 0.83 +0.4 5,466,512 - wcaK JDDGAC_28495 0.83 -0.0 5,466,519 + wcaK JDDGAC_28495 0.83 -1.6 5,466,520 - wcaK JDDGAC_28495 0.83 +1.2 5,466,652 - -0.7 5,466,878 + wcaL JDDGAC_28500 0.12 +0.2 5,466,879 - wcaL JDDGAC_28500 0.12 +1.4 5,466,879 - wcaL JDDGAC_28500 0.12 +0.3 5,467,219 - wcaL JDDGAC_28500 0.40 +1.0 5,467,345 + wcaL JDDGAC_28500 0.50 -0.0 5,467,346 - wcaL JDDGAC_28500 0.51 -0.8 5,467,346 - wcaL JDDGAC_28500 0.51 +0.7 5,467,349 + wcaL JDDGAC_28500 0.51 +2.6 5,467,350 - wcaL JDDGAC_28500 0.51 +0.3 5,467,551 + wcaL JDDGAC_28500 0.67 -0.9 5,467,947 + -1.1 5,467,959 + -1.2 5,467,960 - -0.6 5,468,025 + +0.5 5,468,026 - -0.2 5,468,067 + +0.2 5,468,068 - -2.3 5,468,077 + -1.6 5,468,078 - -1.9 5,468,078 - -0.2 5,468,167 - wcaM JDDGAC_28505 0.15 -0.2 5,468,205 - wcaM JDDGAC_28505 0.18 -0.1 5,468,253 - wcaM JDDGAC_28505 0.21 -0.1 5,468,292 + wcaM JDDGAC_28505 0.24 +0.5 5,468,299 - wcaM JDDGAC_28505 0.24 -1.2 5,468,299 - wcaM JDDGAC_28505 0.24 +0.7 5,468,439 + wcaM JDDGAC_28505 0.34 +1.0 5,468,449 - wcaM JDDGAC_28505 0.35 +1.1 5,468,453 - wcaM JDDGAC_28505 0.35 -0.5 5,468,463 - wcaM JDDGAC_28505 0.36 +1.0 5,468,512 - wcaM JDDGAC_28505 0.40 +0.3 5,468,610 + wcaM JDDGAC_28505 0.47 +0.4 5,468,662 + wcaM JDDGAC_28505 0.50 -0.5 5,468,663 - wcaM JDDGAC_28505 0.50 -1.8 5,468,670 + wcaM JDDGAC_28505 0.51 -0.5 5,468,670 + wcaM JDDGAC_28505 0.51 -1.6 5,468,745 - wcaM JDDGAC_28505 0.56 +1.1 5,468,796 + wcaM JDDGAC_28505 0.60 +2.4 5,468,852 - wcaM JDDGAC_28505 0.64 +0.2 5,468,878 - wcaM JDDGAC_28505 0.66 +0.5 5,468,929 - wcaM JDDGAC_28505 0.69 -1.6
Or see this region's nucleotide sequence