Experiment: WM_JK45
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaC and wcaD overlap by 26 nucleotides wcaD and wcaE are separated by 10 nucleotides wcaE and wcaF are separated by 15 nucleotides
JDDGAC_28435: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 5,451,791 to 5,453,008
wcaC
JDDGAC_28440: wcaD - putative colanic acid polymerase WcaD, at 5,452,983 to 5,454,200
wcaD
JDDGAC_28445: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 5,454,211 to 5,454,957
wcaE
JDDGAC_28450: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 5,454,973 to 5,455,521
wcaF
Position (kb) 5452
5453
5454
5455 Strain fitness (log2 ratio) -2
-1
0
1 at 5451.991 kb on + strand, within wcaC at 5452.045 kb on - strand, within wcaC at 5452.047 kb on - strand, within wcaC at 5452.114 kb on - strand, within wcaC at 5452.279 kb on + strand, within wcaC at 5452.279 kb on + strand, within wcaC at 5452.279 kb on + strand, within wcaC at 5452.280 kb on - strand, within wcaC at 5452.280 kb on - strand, within wcaC at 5452.283 kb on + strand, within wcaC at 5452.438 kb on + strand, within wcaC at 5452.444 kb on + strand, within wcaC at 5452.562 kb on - strand, within wcaC at 5452.616 kb on - strand, within wcaC at 5452.616 kb on - strand, within wcaC at 5452.616 kb on - strand, within wcaC at 5452.658 kb on - strand, within wcaC at 5452.983 kb on - strand at 5453.006 kb on + strand at 5453.006 kb on + strand at 5453.010 kb on + strand at 5453.010 kb on + strand at 5453.032 kb on - strand at 5453.223 kb on + strand, within wcaD at 5453.238 kb on - strand, within wcaD at 5453.238 kb on - strand, within wcaD at 5453.264 kb on + strand, within wcaD at 5453.265 kb on - strand, within wcaD at 5453.292 kb on - strand, within wcaD at 5453.323 kb on + strand, within wcaD at 5453.326 kb on + strand, within wcaD at 5453.326 kb on + strand, within wcaD at 5453.326 kb on + strand, within wcaD at 5453.455 kb on - strand, within wcaD at 5453.592 kb on - strand, within wcaD at 5453.737 kb on + strand, within wcaD at 5454.390 kb on - strand, within wcaE at 5454.390 kb on - strand, within wcaE at 5454.390 kb on - strand, within wcaE at 5455.030 kb on - strand, within wcaF at 5455.085 kb on + strand, within wcaF at 5455.112 kb on + strand, within wcaF
Per-strain Table
Position Strand Gene LocusTag Fraction WM_JK45 remove 5,451,991 + wcaC JDDGAC_28435 0.16 +0.3 5,452,045 - wcaC JDDGAC_28435 0.21 -0.5 5,452,047 - wcaC JDDGAC_28435 0.21 -0.5 5,452,114 - wcaC JDDGAC_28435 0.27 -0.2 5,452,279 + wcaC JDDGAC_28435 0.40 -1.5 5,452,279 + wcaC JDDGAC_28435 0.40 +0.5 5,452,279 + wcaC JDDGAC_28435 0.40 -0.0 5,452,280 - wcaC JDDGAC_28435 0.40 +1.0 5,452,280 - wcaC JDDGAC_28435 0.40 +0.0 5,452,283 + wcaC JDDGAC_28435 0.40 +0.1 5,452,438 + wcaC JDDGAC_28435 0.53 -1.1 5,452,444 + wcaC JDDGAC_28435 0.54 -0.1 5,452,562 - wcaC JDDGAC_28435 0.63 -0.2 5,452,616 - wcaC JDDGAC_28435 0.68 +0.8 5,452,616 - wcaC JDDGAC_28435 0.68 +0.4 5,452,616 - wcaC JDDGAC_28435 0.68 +0.5 5,452,658 - wcaC JDDGAC_28435 0.71 -0.8 5,452,983 - -0.1 5,453,006 + +0.1 5,453,006 + -0.5 5,453,010 + -0.3 5,453,010 + -0.3 5,453,032 - +1.0 5,453,223 + wcaD JDDGAC_28440 0.20 -0.2 5,453,238 - wcaD JDDGAC_28440 0.21 +1.3 5,453,238 - wcaD JDDGAC_28440 0.21 +0.3 5,453,264 + wcaD JDDGAC_28440 0.23 -2.7 5,453,265 - wcaD JDDGAC_28440 0.23 +0.0 5,453,292 - wcaD JDDGAC_28440 0.25 +0.8 5,453,323 + wcaD JDDGAC_28440 0.28 +0.6 5,453,326 + wcaD JDDGAC_28440 0.28 -0.1 5,453,326 + wcaD JDDGAC_28440 0.28 -0.2 5,453,326 + wcaD JDDGAC_28440 0.28 +0.2 5,453,455 - wcaD JDDGAC_28440 0.39 -0.1 5,453,592 - wcaD JDDGAC_28440 0.50 -1.6 5,453,737 + wcaD JDDGAC_28440 0.62 +1.2 5,454,390 - wcaE JDDGAC_28445 0.24 -0.7 5,454,390 - wcaE JDDGAC_28445 0.24 +0.8 5,454,390 - wcaE JDDGAC_28445 0.24 -1.3 5,455,030 - wcaF JDDGAC_28450 0.10 +0.2 5,455,085 + wcaF JDDGAC_28450 0.20 -1.6 5,455,112 + wcaF JDDGAC_28450 0.25 +0.1
Or see this region's nucleotide sequence