Experiment: WM_JK45
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt guaC and yacM are separated by 155 nucleotides yacM and coaE are separated by 24 nucleotides coaE and zapD overlap by 1 nucleotides zapD and yacG are separated by 9 nucleotides yacG and mutT are separated by 90 nucleotides mutT and secA are separated by 59 nucleotides
JDDGAC_14145: guaC - GMP reductase, at 2,627,013 to 2,628,056
guaC
JDDGAC_14150: yacM - Protein YacM, at 2,628,212 to 2,628,256
yacM
JDDGAC_14155: coaE - dephospho-CoA kinase, at 2,628,281 to 2,628,901
coaE
JDDGAC_14160: zapD - cell division protein ZapD, at 2,628,901 to 2,629,644
zapD
JDDGAC_14165: yacG - DNA gyrase inhibitor YacG, at 2,629,654 to 2,629,851
yacG
JDDGAC_14175: mutT - 8-oxo-dGTP diphosphatase MutT, at 2,629,942 to 2,630,340
mutT
JDDGAC_14185: secA - preprotein translocase subunit SecA, at 2,630,400 to 2,633,105
secA
Position (kb)
2628
2629
2630 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2627.987 kb on + strand at 2628.031 kb on - strand at 2628.050 kb on + strand at 2628.051 kb on - strand at 2628.082 kb on - strand at 2628.082 kb on - strand at 2628.082 kb on - strand at 2628.082 kb on - strand at 2628.090 kb on - strand at 2628.118 kb on - strand at 2628.167 kb on + strand at 2628.190 kb on + strand at 2628.191 kb on - strand at 2628.275 kb on + strand at 2628.280 kb on + strand at 2628.291 kb on + strand at 2628.291 kb on + strand at 2628.291 kb on + strand at 2628.291 kb on + strand at 2628.292 kb on - strand at 2628.933 kb on - strand at 2628.949 kb on - strand at 2628.949 kb on - strand at 2628.949 kb on - strand at 2628.949 kb on - strand at 2629.205 kb on + strand, within zapD at 2629.307 kb on + strand, within zapD at 2629.311 kb on + strand, within zapD at 2629.311 kb on + strand, within zapD at 2629.404 kb on + strand, within zapD at 2629.404 kb on + strand, within zapD at 2629.404 kb on + strand, within zapD at 2629.405 kb on - strand, within zapD at 2629.405 kb on - strand, within zapD at 2629.471 kb on + strand, within zapD at 2629.471 kb on + strand, within zapD at 2629.554 kb on + strand, within zapD at 2629.554 kb on + strand, within zapD at 2629.554 kb on + strand, within zapD at 2629.555 kb on - strand, within zapD at 2629.555 kb on - strand, within zapD at 2629.579 kb on - strand at 2629.643 kb on - strand at 2629.669 kb on - strand at 2629.952 kb on + strand at 2629.953 kb on - strand at 2629.953 kb on - strand at 2629.953 kb on - strand at 2629.962 kb on + strand at 2629.962 kb on + strand at 2629.962 kb on + strand at 2629.975 kb on + strand at 2630.101 kb on + strand, within mutT at 2630.101 kb on + strand, within mutT at 2630.101 kb on + strand, within mutT at 2630.101 kb on + strand, within mutT at 2630.102 kb on - strand, within mutT at 2630.102 kb on - strand, within mutT at 2630.102 kb on - strand, within mutT at 2630.102 kb on - strand, within mutT at 2630.102 kb on - strand, within mutT at 2630.102 kb on - strand, within mutT at 2630.102 kb on - strand, within mutT at 2630.103 kb on + strand, within mutT at 2630.103 kb on + strand, within mutT at 2630.104 kb on - strand, within mutT at 2630.104 kb on - strand, within mutT at 2630.104 kb on - strand, within mutT at 2630.104 kb on - strand, within mutT at 2630.104 kb on - strand, within mutT at 2630.248 kb on - strand, within mutT at 2630.313 kb on + strand at 2630.314 kb on - strand at 2630.347 kb on - strand at 2630.353 kb on - strand at 2630.383 kb on - strand at 2630.402 kb on - strand at 2630.469 kb on + strand at 2630.470 kb on - strand at 2630.470 kb on - strand at 2630.534 kb on + strand at 2630.535 kb on - strand at 2630.610 kb on - strand at 2630.610 kb on - strand at 2630.610 kb on - strand at 2630.610 kb on - strand at 2630.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_JK45 remove 2,627,987 + +0.0 2,628,031 - -1.6 2,628,050 + +1.8 2,628,051 - -0.7 2,628,082 - -0.7 2,628,082 - +2.3 2,628,082 - +1.3 2,628,082 - +0.6 2,628,090 - -0.4 2,628,118 - +1.0 2,628,167 + +0.1 2,628,190 + +0.6 2,628,191 - -0.6 2,628,275 + +0.5 2,628,280 + -1.0 2,628,291 + -1.8 2,628,291 + +0.7 2,628,291 + -0.5 2,628,291 + -1.2 2,628,292 - -0.4 2,628,933 - -0.4 2,628,949 - +0.3 2,628,949 - +0.3 2,628,949 - +0.2 2,628,949 - +0.7 2,629,205 + zapD JDDGAC_14160 0.41 +0.4 2,629,307 + zapD JDDGAC_14160 0.55 -1.0 2,629,311 + zapD JDDGAC_14160 0.55 +0.8 2,629,311 + zapD JDDGAC_14160 0.55 -0.2 2,629,404 + zapD JDDGAC_14160 0.68 -1.5 2,629,404 + zapD JDDGAC_14160 0.68 -0.6 2,629,404 + zapD JDDGAC_14160 0.68 -0.3 2,629,405 - zapD JDDGAC_14160 0.68 +2.4 2,629,405 - zapD JDDGAC_14160 0.68 -3.1 2,629,471 + zapD JDDGAC_14160 0.77 +0.9 2,629,471 + zapD JDDGAC_14160 0.77 +1.6 2,629,554 + zapD JDDGAC_14160 0.88 -2.3 2,629,554 + zapD JDDGAC_14160 0.88 -0.0 2,629,554 + zapD JDDGAC_14160 0.88 -0.4 2,629,555 - zapD JDDGAC_14160 0.88 +0.1 2,629,555 - zapD JDDGAC_14160 0.88 +0.3 2,629,579 - +1.0 2,629,643 - +0.4 2,629,669 - -0.5 2,629,952 + +0.1 2,629,953 - +1.8 2,629,953 - -0.7 2,629,953 - +1.5 2,629,962 + +1.1 2,629,962 + +0.3 2,629,962 + +0.2 2,629,975 + -1.0 2,630,101 + mutT JDDGAC_14175 0.40 -1.3 2,630,101 + mutT JDDGAC_14175 0.40 +0.2 2,630,101 + mutT JDDGAC_14175 0.40 -1.1 2,630,101 + mutT JDDGAC_14175 0.40 -0.1 2,630,102 - mutT JDDGAC_14175 0.40 +0.5 2,630,102 - mutT JDDGAC_14175 0.40 +0.1 2,630,102 - mutT JDDGAC_14175 0.40 -0.6 2,630,102 - mutT JDDGAC_14175 0.40 +0.5 2,630,102 - mutT JDDGAC_14175 0.40 +1.1 2,630,102 - mutT JDDGAC_14175 0.40 -0.6 2,630,102 - mutT JDDGAC_14175 0.40 -0.7 2,630,103 + mutT JDDGAC_14175 0.40 -1.3 2,630,103 + mutT JDDGAC_14175 0.40 +2.3 2,630,104 - mutT JDDGAC_14175 0.41 -1.2 2,630,104 - mutT JDDGAC_14175 0.41 -0.1 2,630,104 - mutT JDDGAC_14175 0.41 -0.6 2,630,104 - mutT JDDGAC_14175 0.41 -0.2 2,630,104 - mutT JDDGAC_14175 0.41 +0.4 2,630,248 - mutT JDDGAC_14175 0.77 -0.7 2,630,313 + -0.4 2,630,314 - -0.8 2,630,347 - -1.8 2,630,353 - -0.5 2,630,383 - -2.9 2,630,402 - +0.7 2,630,469 + -0.3 2,630,470 - +0.1 2,630,470 - +0.6 2,630,534 + -0.9 2,630,535 - -1.0 2,630,610 - -2.5 2,630,610 - -0.1 2,630,610 - -0.8 2,630,610 - +0.6 2,630,614 - -0.6
Or see this region's nucleotide sequence