Experiment: WM_Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaA and wcaB are separated by 2 nucleotides wcaB and wcaC overlap by 4 nucleotides wcaC and wcaD overlap by 26 nucleotides
JDDGAC_28425: wcaA - colanic acid biosynthesis glycosyltransferase WcaA, at 5,450,464 to 5,451,303
wcaA
JDDGAC_28430: wcaB - colanic acid biosynthesis acetyltransferase WcaB, at 5,451,306 to 5,451,794
wcaB
JDDGAC_28435: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 5,451,791 to 5,453,008
wcaC
JDDGAC_28440: wcaD - putative colanic acid polymerase WcaD, at 5,452,983 to 5,454,200
wcaD
Position (kb) 5451
5452
5453
5454 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5450.834 kb on - strand, within wcaA at 5450.873 kb on + strand, within wcaA at 5450.873 kb on + strand, within wcaA at 5450.874 kb on - strand, within wcaA at 5450.926 kb on - strand, within wcaA at 5450.926 kb on - strand, within wcaA at 5451.007 kb on + strand, within wcaA at 5451.133 kb on + strand, within wcaA at 5451.133 kb on + strand, within wcaA at 5451.134 kb on - strand, within wcaA at 5451.306 kb on - strand at 5451.516 kb on - strand, within wcaB at 5451.548 kb on + strand, within wcaB at 5451.549 kb on - strand, within wcaB at 5451.808 kb on + strand at 5451.964 kb on + strand, within wcaC at 5451.991 kb on + strand, within wcaC at 5452.045 kb on - strand, within wcaC at 5452.047 kb on - strand, within wcaC at 5452.114 kb on - strand, within wcaC at 5452.279 kb on + strand, within wcaC at 5452.279 kb on + strand, within wcaC at 5452.279 kb on + strand, within wcaC at 5452.280 kb on - strand, within wcaC at 5452.280 kb on - strand, within wcaC at 5452.283 kb on + strand, within wcaC at 5452.438 kb on + strand, within wcaC at 5452.444 kb on + strand, within wcaC at 5452.562 kb on - strand, within wcaC at 5452.616 kb on - strand, within wcaC at 5452.616 kb on - strand, within wcaC at 5452.616 kb on - strand, within wcaC at 5452.658 kb on - strand, within wcaC at 5452.983 kb on - strand at 5453.006 kb on + strand at 5453.006 kb on + strand at 5453.010 kb on + strand at 5453.010 kb on + strand at 5453.032 kb on - strand at 5453.223 kb on + strand, within wcaD at 5453.238 kb on - strand, within wcaD at 5453.238 kb on - strand, within wcaD at 5453.264 kb on + strand, within wcaD at 5453.265 kb on - strand, within wcaD at 5453.292 kb on - strand, within wcaD at 5453.323 kb on + strand, within wcaD at 5453.326 kb on + strand, within wcaD at 5453.326 kb on + strand, within wcaD at 5453.326 kb on + strand, within wcaD at 5453.455 kb on - strand, within wcaD at 5453.592 kb on - strand, within wcaD at 5453.737 kb on + strand, within wcaD
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas09 remove 5,450,834 - wcaA JDDGAC_28425 0.44 -0.8 5,450,873 + wcaA JDDGAC_28425 0.49 -0.9 5,450,873 + wcaA JDDGAC_28425 0.49 -0.2 5,450,874 - wcaA JDDGAC_28425 0.49 -1.7 5,450,926 - wcaA JDDGAC_28425 0.55 -0.4 5,450,926 - wcaA JDDGAC_28425 0.55 +0.3 5,451,007 + wcaA JDDGAC_28425 0.65 -0.0 5,451,133 + wcaA JDDGAC_28425 0.80 +0.3 5,451,133 + wcaA JDDGAC_28425 0.80 -0.1 5,451,134 - wcaA JDDGAC_28425 0.80 -0.9 5,451,306 - -0.3 5,451,516 - wcaB JDDGAC_28430 0.43 -0.7 5,451,548 + wcaB JDDGAC_28430 0.49 -0.3 5,451,549 - wcaB JDDGAC_28430 0.50 +0.9 5,451,808 + +0.6 5,451,964 + wcaC JDDGAC_28435 0.14 -1.0 5,451,991 + wcaC JDDGAC_28435 0.16 -3.2 5,452,045 - wcaC JDDGAC_28435 0.21 -0.0 5,452,047 - wcaC JDDGAC_28435 0.21 -1.4 5,452,114 - wcaC JDDGAC_28435 0.27 -1.1 5,452,279 + wcaC JDDGAC_28435 0.40 -2.2 5,452,279 + wcaC JDDGAC_28435 0.40 -3.0 5,452,279 + wcaC JDDGAC_28435 0.40 +0.1 5,452,280 - wcaC JDDGAC_28435 0.40 +1.1 5,452,280 - wcaC JDDGAC_28435 0.40 +0.5 5,452,283 + wcaC JDDGAC_28435 0.40 -1.3 5,452,438 + wcaC JDDGAC_28435 0.53 -1.0 5,452,444 + wcaC JDDGAC_28435 0.54 -0.1 5,452,562 - wcaC JDDGAC_28435 0.63 +0.0 5,452,616 - wcaC JDDGAC_28435 0.68 -0.7 5,452,616 - wcaC JDDGAC_28435 0.68 +0.7 5,452,616 - wcaC JDDGAC_28435 0.68 +0.8 5,452,658 - wcaC JDDGAC_28435 0.71 -0.4 5,452,983 - +0.2 5,453,006 + -0.3 5,453,006 + +0.7 5,453,010 + -2.5 5,453,010 + -1.5 5,453,032 - +0.7 5,453,223 + wcaD JDDGAC_28440 0.20 +1.3 5,453,238 - wcaD JDDGAC_28440 0.21 +0.9 5,453,238 - wcaD JDDGAC_28440 0.21 +1.1 5,453,264 + wcaD JDDGAC_28440 0.23 -1.6 5,453,265 - wcaD JDDGAC_28440 0.23 -0.9 5,453,292 - wcaD JDDGAC_28440 0.25 +0.4 5,453,323 + wcaD JDDGAC_28440 0.28 +0.6 5,453,326 + wcaD JDDGAC_28440 0.28 +0.5 5,453,326 + wcaD JDDGAC_28440 0.28 -0.1 5,453,326 + wcaD JDDGAC_28440 0.28 -1.4 5,453,455 - wcaD JDDGAC_28440 0.39 -0.0 5,453,592 - wcaD JDDGAC_28440 0.50 +0.4 5,453,737 + wcaD JDDGAC_28440 0.62 +1.2
Or see this region's nucleotide sequence