Strain Fitness in Escherichia coli ECRC98 around JDDGAC_27680

Experiment: WM_Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntJDDGAC_27665 and JDDGAC_27670 overlap by 4 nucleotidesJDDGAC_27670 and JDDGAC_27675 are separated by 333 nucleotidesJDDGAC_27675 and JDDGAC_27680 are separated by 1 nucleotidesJDDGAC_27680 and JDDGAC_27685 overlap by 4 nucleotidesJDDGAC_27685 and JDDGAC_27690 are separated by 98 nucleotidesJDDGAC_27690 and JDDGAC_27695 overlap by 4 nucleotidesJDDGAC_27695 and JDDGAC_27700 overlap by 4 nucleotidesJDDGAC_27700 and JDDGAC_27705 overlap by 28 nucleotidesJDDGAC_27705 and exoD overlap by 1 nucleotides JDDGAC_27665: JDDGAC_27665 - DUF551 domain-containing protein, at 5,319,156 to 5,319,518 _27665 JDDGAC_27670: JDDGAC_27670 - Phage protein, at 5,319,515 to 5,319,871 _27670 JDDGAC_27675: JDDGAC_27675 - hypothetical protein, at 5,320,205 to 5,320,381 _27675 JDDGAC_27680: JDDGAC_27680 - Ead/Ea22-like protein, at 5,320,383 to 5,321,330 _27680 JDDGAC_27685: JDDGAC_27685 - conjugal transfer protein TraR, at 5,321,327 to 5,321,548 _27685 JDDGAC_27690: JDDGAC_27690 - Cell division protein ZapA, at 5,321,647 to 5,321,862 _27690 JDDGAC_27695: JDDGAC_27695 - DUF1382 domain-containing protein, at 5,321,859 to 5,321,942 _27695 JDDGAC_27700: JDDGAC_27700 - DUF1382 domain-containing protein, at 5,321,939 to 5,322,130 _27700 JDDGAC_27705: JDDGAC_27705 - cruciferin, at 5,322,103 to 5,322,285 _27705 JDDGAC_27710: exoD - Putative uncharacterized protein ExoD, at 5,322,285 to 5,322,962 exoD Position (kb) 5320 5321 5322Strain fitness (log2 ratio) -1 0 1at 5321.163 kb on - strand, within JDDGAC_27680at 5321.163 kb on - strand, within JDDGAC_27680at 5322.130 kb on + strandat 5322.147 kb on + strand, within JDDGAC_27705at 5322.148 kb on - strand, within JDDGAC_27705at 5322.286 kb on + strandat 5322.304 kb on - strandat 5322.304 kb on - strandat 5322.304 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas09
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5,321,163 - JDDGAC_27680 0.82 +0.7
5,321,163 - JDDGAC_27680 0.82 +0.1
5,322,130 + +0.8
5,322,147 + JDDGAC_27705 0.24 +1.1
5,322,148 - JDDGAC_27705 0.25 +0.7
5,322,286 + -0.4
5,322,304 - +0.7
5,322,304 - -0.5
5,322,304 - +1.4

Or see this region's nucleotide sequence