Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS12985 and A4249_RS12990 overlap by 8 nucleotides A4249_RS12990 and A4249_RS12995 are separated by 42 nucleotides A4249_RS12995 and A4249_RS13000 overlap by 4 nucleotides A4249_RS13000 and A4249_RS13005 overlap by 19 nucleotides
A4249_RS12985: A4249_RS12985 - GDP-mannose 4,6-dehydratase, at 2,605,075 to 2,606,067
_RS12985
A4249_RS12990: A4249_RS12990 - ABC transporter permease, at 2,606,060 to 2,606,878
_RS12990
A4249_RS12995: A4249_RS12995 - chain-length determining protein, at 2,606,921 to 2,608,084
_RS12995
A4249_RS13000: A4249_RS13000 - ATP-binding cassette domain-containing protein, at 2,608,081 to 2,608,761
_RS13000
A4249_RS13005: A4249_RS13005 - hypothetical protein, at 2,608,743 to 2,609,663
_RS13005
Position (kb)
2606
2607
2608
2609 Strain fitness (log2 ratio)
-1
0
1 at 2606.026 kb on + strand at 2606.026 kb on + strand at 2606.054 kb on + strand at 2606.876 kb on + strand at 2606.894 kb on + strand at 2606.906 kb on + strand at 2606.906 kb on + strand at 2606.906 kb on + strand at 2606.906 kb on + strand at 2606.906 kb on + strand at 2606.906 kb on + strand at 2606.906 kb on + strand at 2606.915 kb on + strand at 2606.915 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 2,606,026 + +1.1 2,606,026 + +0.2 2,606,054 + +0.3 2,606,876 + -0.3 2,606,894 + +0.1 2,606,906 + -0.2 2,606,906 + -0.4 2,606,906 + -0.9 2,606,906 + +0.9 2,606,906 + +1.4 2,606,906 + -0.9 2,606,906 + -0.1 2,606,915 + +0.4 2,606,915 + -0.2
Or see this region's nucleotide sequence