Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS12925 and A4249_RS12930 are separated by 42 nucleotides A4249_RS12930 and A4249_RS12935 are separated by 56 nucleotides A4249_RS12935 and A4249_RS16370 are separated by 79 nucleotides A4249_RS16370 and A4249_RS12940 are separated by 3 nucleotides
A4249_RS12925: A4249_RS12925 - tetratricopeptide repeat protein, at 2,592,060 to 2,592,896
_RS12925
A4249_RS12930: A4249_RS12930 - HAD-IA family hydrolase, at 2,592,939 to 2,593,598
_RS12930
A4249_RS12935: A4249_RS12935 - iron-sulfur cluster assembly scaffold protein, at 2,593,655 to 2,594,110
_RS12935
A4249_RS16370: A4249_RS16370 - membrane protein insertion efficiency factor YidD, at 2,594,190 to 2,594,420
_RS16370
A4249_RS12940: A4249_RS12940 - threonine--tRNA ligase, at 2,594,424 to 2,596,352
_RS12940
Position (kb)
2593
2594
2595 Strain fitness (log2 ratio)
-1
0
1 at 2592.966 kb on + strand at 2592.966 kb on + strand at 2593.136 kb on + strand, within A4249_RS12930 at 2593.377 kb on + strand, within A4249_RS12930 at 2593.542 kb on + strand at 2593.542 kb on + strand at 2593.596 kb on + strand at 2593.597 kb on - strand at 2593.597 kb on - strand at 2593.643 kb on + strand at 2593.643 kb on + strand at 2593.644 kb on - strand at 2593.644 kb on - strand at 2593.644 kb on - strand at 2593.644 kb on - strand at 2594.149 kb on + strand at 2594.149 kb on + strand at 2594.199 kb on + strand at 2594.199 kb on + strand at 2594.199 kb on + strand at 2594.200 kb on - strand at 2594.411 kb on + strand at 2594.412 kb on - strand at 2594.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 2,592,966 + -1.4 2,592,966 + +1.0 2,593,136 + A4249_RS12930 0.30 -0.0 2,593,377 + A4249_RS12930 0.66 +1.2 2,593,542 + +0.5 2,593,542 + -0.4 2,593,596 + -0.4 2,593,597 - -0.8 2,593,597 - -0.0 2,593,643 + +0.4 2,593,643 + +0.4 2,593,644 - -0.8 2,593,644 - -0.9 2,593,644 - -0.1 2,593,644 - +0.8 2,594,149 + -1.2 2,594,149 + -0.4 2,594,199 + +0.6 2,594,199 + +1.2 2,594,199 + -1.4 2,594,200 - -0.5 2,594,411 + -0.0 2,594,412 - +0.1 2,594,412 - -0.6
Or see this region's nucleotide sequence