Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS09895 and A4249_RS09900 are separated by 68 nucleotides A4249_RS09900 and A4249_RS09905 are separated by 60 nucleotides A4249_RS09905 and A4249_RS09910 overlap by 4 nucleotides
A4249_RS09890: A4249_RS09890 - FtsQ-type POTRA domain-containing protein, at 1,973,076 to 1,973,963
_RS09890
A4249_RS09895: A4249_RS09895 - D-alanine--D-alanine ligase, at 1,973,951 to 1,974,892
_RS09895
A4249_RS09900: A4249_RS09900 - hypothetical protein, at 1,974,961 to 1,976,241
_RS09900
A4249_RS09905: A4249_RS09905 - UDP-N-acetylmuramate dehydrogenase, at 1,976,302 to 1,977,201
_RS09905
A4249_RS09910: A4249_RS09910 - UDP-N-acetylmuramate--L-alanine ligase, at 1,977,198 to 1,978,610
_RS09910
Position (kb) 1974
1975
1976
1977 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1975.079 kb on + strand at 1975.080 kb on - strand at 1975.085 kb on + strand at 1975.085 kb on + strand at 1975.085 kb on + strand at 1975.085 kb on + strand at 1975.085 kb on + strand at 1975.085 kb on + strand at 1975.085 kb on + strand at 1975.085 kb on + strand at 1975.085 kb on + strand at 1975.086 kb on - strand at 1975.086 kb on - strand at 1975.086 kb on - strand at 1975.086 kb on - strand at 1975.086 kb on - strand at 1975.086 kb on - strand at 1975.086 kb on - strand at 1975.086 kb on - strand at 1975.086 kb on - strand at 1975.086 kb on - strand at 1975.086 kb on - strand at 1975.086 kb on - strand at 1975.086 kb on - strand at 1975.086 kb on - strand at 1975.115 kb on + strand, within A4249_RS09900 at 1975.116 kb on - strand, within A4249_RS09900 at 1975.241 kb on + strand, within A4249_RS09900 at 1975.241 kb on + strand, within A4249_RS09900 at 1975.241 kb on + strand, within A4249_RS09900 at 1975.241 kb on + strand, within A4249_RS09900 at 1975.241 kb on + strand, within A4249_RS09900 at 1975.241 kb on + strand, within A4249_RS09900 at 1975.288 kb on + strand, within A4249_RS09900 at 1975.301 kb on + strand, within A4249_RS09900 at 1975.301 kb on + strand, within A4249_RS09900 at 1975.301 kb on + strand, within A4249_RS09900 at 1975.301 kb on + strand, within A4249_RS09900 at 1975.301 kb on + strand, within A4249_RS09900 at 1975.301 kb on + strand, within A4249_RS09900 at 1975.301 kb on + strand, within A4249_RS09900 at 1975.301 kb on + strand, within A4249_RS09900 at 1975.302 kb on - strand, within A4249_RS09900 at 1975.302 kb on - strand, within A4249_RS09900 at 1975.302 kb on - strand, within A4249_RS09900 at 1975.302 kb on - strand, within A4249_RS09900 at 1975.302 kb on - strand, within A4249_RS09900 at 1975.302 kb on - strand, within A4249_RS09900 at 1975.302 kb on - strand, within A4249_RS09900 at 1975.302 kb on - strand, within A4249_RS09900 at 1975.480 kb on - strand, within A4249_RS09900 at 1975.574 kb on - strand, within A4249_RS09900 at 1975.618 kb on - strand, within A4249_RS09900 at 1975.736 kb on + strand, within A4249_RS09900 at 1975.736 kb on + strand, within A4249_RS09900 at 1975.736 kb on + strand, within A4249_RS09900 at 1975.736 kb on + strand, within A4249_RS09900 at 1975.736 kb on + strand, within A4249_RS09900 at 1975.736 kb on + strand, within A4249_RS09900 at 1975.736 kb on + strand, within A4249_RS09900 at 1975.737 kb on - strand, within A4249_RS09900 at 1975.737 kb on - strand, within A4249_RS09900 at 1975.737 kb on - strand, within A4249_RS09900 at 1976.093 kb on + strand, within A4249_RS09900 at 1976.093 kb on + strand, within A4249_RS09900 at 1976.093 kb on + strand, within A4249_RS09900 at 1976.093 kb on + strand, within A4249_RS09900 at 1976.093 kb on + strand, within A4249_RS09900 at 1976.093 kb on + strand, within A4249_RS09900 at 1976.093 kb on + strand, within A4249_RS09900 at 1976.093 kb on + strand, within A4249_RS09900 at 1976.093 kb on + strand, within A4249_RS09900 at 1976.093 kb on + strand, within A4249_RS09900 at 1976.094 kb on - strand, within A4249_RS09900 at 1976.094 kb on - strand, within A4249_RS09900 at 1976.094 kb on - strand, within A4249_RS09900 at 1976.094 kb on - strand, within A4249_RS09900 at 1976.094 kb on - strand, within A4249_RS09900 at 1976.094 kb on - strand, within A4249_RS09900 at 1976.094 kb on - strand, within A4249_RS09900 at 1976.094 kb on - strand, within A4249_RS09900 at 1976.094 kb on - strand, within A4249_RS09900 at 1976.094 kb on - strand, within A4249_RS09900 at 1976.094 kb on - strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.097 kb on + strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.098 kb on - strand, within A4249_RS09900 at 1976.099 kb on + strand, within A4249_RS09900 at 1976.099 kb on + strand, within A4249_RS09900 at 1976.099 kb on + strand, within A4249_RS09900 at 1976.099 kb on - strand, within A4249_RS09900 at 1976.100 kb on - strand, within A4249_RS09900 at 1976.100 kb on - strand, within A4249_RS09900 at 1976.100 kb on - strand, within A4249_RS09900 at 1976.100 kb on - strand, within A4249_RS09900 at 1976.270 kb on + strand at 1976.270 kb on + strand at 1976.271 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 1,975,079 + +0.7 1,975,080 - +0.1 1,975,085 + +0.1 1,975,085 + +0.1 1,975,085 + -0.1 1,975,085 + +0.1 1,975,085 + +0.4 1,975,085 + +0.3 1,975,085 + +0.5 1,975,085 + -0.3 1,975,085 + -0.5 1,975,086 - -0.3 1,975,086 - -0.3 1,975,086 - -1.5 1,975,086 - +0.3 1,975,086 - -0.3 1,975,086 - +1.1 1,975,086 - -0.0 1,975,086 - -0.7 1,975,086 - +0.0 1,975,086 - -0.3 1,975,086 - +0.3 1,975,086 - -0.2 1,975,086 - +0.2 1,975,086 - +0.2 1,975,115 + A4249_RS09900 0.12 -0.0 1,975,116 - A4249_RS09900 0.12 +0.5 1,975,241 + A4249_RS09900 0.22 +0.9 1,975,241 + A4249_RS09900 0.22 +0.2 1,975,241 + A4249_RS09900 0.22 -1.3 1,975,241 + A4249_RS09900 0.22 -0.3 1,975,241 + A4249_RS09900 0.22 +0.9 1,975,241 + A4249_RS09900 0.22 +0.3 1,975,288 + A4249_RS09900 0.26 -0.6 1,975,301 + A4249_RS09900 0.27 +0.7 1,975,301 + A4249_RS09900 0.27 -1.5 1,975,301 + A4249_RS09900 0.27 +0.2 1,975,301 + A4249_RS09900 0.27 +0.3 1,975,301 + A4249_RS09900 0.27 +0.1 1,975,301 + A4249_RS09900 0.27 -0.2 1,975,301 + A4249_RS09900 0.27 +0.4 1,975,301 + A4249_RS09900 0.27 -0.2 1,975,302 - A4249_RS09900 0.27 -0.3 1,975,302 - A4249_RS09900 0.27 +0.0 1,975,302 - A4249_RS09900 0.27 +0.7 1,975,302 - A4249_RS09900 0.27 -0.1 1,975,302 - A4249_RS09900 0.27 -0.4 1,975,302 - A4249_RS09900 0.27 +1.4 1,975,302 - A4249_RS09900 0.27 -1.0 1,975,302 - A4249_RS09900 0.27 +0.2 1,975,480 - A4249_RS09900 0.41 -0.7 1,975,574 - A4249_RS09900 0.48 -0.0 1,975,618 - A4249_RS09900 0.51 -0.0 1,975,736 + A4249_RS09900 0.60 +0.0 1,975,736 + A4249_RS09900 0.60 -0.5 1,975,736 + A4249_RS09900 0.60 +0.9 1,975,736 + A4249_RS09900 0.60 +0.5 1,975,736 + A4249_RS09900 0.60 +1.1 1,975,736 + A4249_RS09900 0.60 -0.7 1,975,736 + A4249_RS09900 0.60 -0.1 1,975,737 - A4249_RS09900 0.61 -0.3 1,975,737 - A4249_RS09900 0.61 -1.2 1,975,737 - A4249_RS09900 0.61 -0.9 1,976,093 + A4249_RS09900 0.88 -0.2 1,976,093 + A4249_RS09900 0.88 -0.7 1,976,093 + A4249_RS09900 0.88 +0.1 1,976,093 + A4249_RS09900 0.88 -0.4 1,976,093 + A4249_RS09900 0.88 -0.2 1,976,093 + A4249_RS09900 0.88 -0.5 1,976,093 + A4249_RS09900 0.88 -0.1 1,976,093 + A4249_RS09900 0.88 +0.1 1,976,093 + A4249_RS09900 0.88 -0.2 1,976,093 + A4249_RS09900 0.88 +0.1 1,976,094 - A4249_RS09900 0.88 -1.1 1,976,094 - A4249_RS09900 0.88 -0.8 1,976,094 - A4249_RS09900 0.88 -0.3 1,976,094 - A4249_RS09900 0.88 +1.0 1,976,094 - A4249_RS09900 0.88 -0.1 1,976,094 - A4249_RS09900 0.88 -0.9 1,976,094 - A4249_RS09900 0.88 +0.7 1,976,094 - A4249_RS09900 0.88 -0.1 1,976,094 - A4249_RS09900 0.88 -0.6 1,976,094 - A4249_RS09900 0.88 +1.2 1,976,094 - A4249_RS09900 0.88 -0.3 1,976,097 + A4249_RS09900 0.89 -0.1 1,976,097 + A4249_RS09900 0.89 -2.8 1,976,097 + A4249_RS09900 0.89 -0.6 1,976,097 + A4249_RS09900 0.89 -0.5 1,976,097 + A4249_RS09900 0.89 -0.0 1,976,097 + A4249_RS09900 0.89 +0.4 1,976,097 + A4249_RS09900 0.89 +1.0 1,976,097 + A4249_RS09900 0.89 +0.1 1,976,097 + A4249_RS09900 0.89 +0.1 1,976,097 + A4249_RS09900 0.89 -0.3 1,976,097 + A4249_RS09900 0.89 +0.7 1,976,097 + A4249_RS09900 0.89 -0.2 1,976,097 + A4249_RS09900 0.89 -0.8 1,976,097 + A4249_RS09900 0.89 +0.4 1,976,097 + A4249_RS09900 0.89 -0.4 1,976,098 - A4249_RS09900 0.89 +0.3 1,976,098 - A4249_RS09900 0.89 -0.1 1,976,098 - A4249_RS09900 0.89 -0.3 1,976,098 - A4249_RS09900 0.89 -0.1 1,976,098 - A4249_RS09900 0.89 +0.2 1,976,098 - A4249_RS09900 0.89 -0.0 1,976,098 - A4249_RS09900 0.89 -0.6 1,976,098 - A4249_RS09900 0.89 +0.7 1,976,098 - A4249_RS09900 0.89 -0.3 1,976,098 - A4249_RS09900 0.89 -0.1 1,976,098 - A4249_RS09900 0.89 +0.9 1,976,098 - A4249_RS09900 0.89 +0.3 1,976,098 - A4249_RS09900 0.89 -0.4 1,976,098 - A4249_RS09900 0.89 +0.2 1,976,098 - A4249_RS09900 0.89 +0.4 1,976,098 - A4249_RS09900 0.89 +0.6 1,976,098 - A4249_RS09900 0.89 -0.9 1,976,098 - A4249_RS09900 0.89 +0.5 1,976,098 - A4249_RS09900 0.89 +0.8 1,976,099 + A4249_RS09900 0.89 -1.5 1,976,099 + A4249_RS09900 0.89 -0.4 1,976,099 + A4249_RS09900 0.89 -0.0 1,976,099 - A4249_RS09900 0.89 -0.2 1,976,100 - A4249_RS09900 0.89 -0.7 1,976,100 - A4249_RS09900 0.89 +0.0 1,976,100 - A4249_RS09900 0.89 +1.0 1,976,100 - A4249_RS09900 0.89 +0.5 1,976,270 + -0.8 1,976,270 + +0.3 1,976,271 - -2.2
Or see this region's nucleotide sequence