Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS08735 and A4249_RS08740 are separated by 61 nucleotides A4249_RS08740 and A4249_RS08745 are separated by 77 nucleotides A4249_RS08745 and A4249_RS08750 are separated by 60 nucleotides
A4249_RS08735: A4249_RS08735 - helix-turn-helix transcriptional regulator, at 1,753,163 to 1,753,798
_RS08735
A4249_RS08740: A4249_RS08740 - SH3 domain-containing protein, at 1,753,860 to 1,754,333
_RS08740
A4249_RS08745: A4249_RS08745 - hypothetical protein, at 1,754,411 to 1,754,800
_RS08745
A4249_RS08750: A4249_RS08750 - lysine--tRNA ligase, at 1,754,861 to 1,756,543
_RS08750
Position (kb)
1754
1755 Strain fitness (log2 ratio)
-1
0
1 at 1753.796 kb on + strand at 1753.796 kb on + strand at 1753.876 kb on + strand at 1753.876 kb on + strand at 1753.876 kb on + strand at 1753.876 kb on + strand at 1753.876 kb on + strand at 1753.877 kb on - strand at 1753.877 kb on - strand at 1753.877 kb on - strand at 1753.877 kb on - strand at 1753.877 kb on - strand at 1753.978 kb on + strand, within A4249_RS08740 at 1753.978 kb on + strand, within A4249_RS08740 at 1753.979 kb on - strand, within A4249_RS08740 at 1753.979 kb on - strand, within A4249_RS08740 at 1753.979 kb on - strand, within A4249_RS08740 at 1754.040 kb on - strand, within A4249_RS08740 at 1754.083 kb on + strand, within A4249_RS08740 at 1754.083 kb on + strand, within A4249_RS08740 at 1754.083 kb on + strand, within A4249_RS08740 at 1754.083 kb on + strand, within A4249_RS08740 at 1754.084 kb on - strand, within A4249_RS08740 at 1754.084 kb on - strand, within A4249_RS08740 at 1754.084 kb on - strand, within A4249_RS08740 at 1754.084 kb on - strand, within A4249_RS08740 at 1754.084 kb on - strand, within A4249_RS08740 at 1754.084 kb on - strand, within A4249_RS08740 at 1754.132 kb on + strand, within A4249_RS08740 at 1754.132 kb on + strand, within A4249_RS08740 at 1754.132 kb on + strand, within A4249_RS08740 at 1754.133 kb on - strand, within A4249_RS08740 at 1754.333 kb on + strand at 1754.333 kb on + strand at 1754.333 kb on + strand at 1754.333 kb on + strand at 1754.333 kb on + strand at 1754.333 kb on + strand at 1754.334 kb on - strand at 1754.334 kb on - strand at 1754.334 kb on - strand at 1754.334 kb on - strand at 1754.334 kb on - strand at 1754.334 kb on - strand at 1754.334 kb on - strand at 1754.432 kb on + strand at 1754.695 kb on + strand, within A4249_RS08745 at 1754.695 kb on + strand, within A4249_RS08745 at 1754.696 kb on - strand, within A4249_RS08745 at 1754.696 kb on - strand, within A4249_RS08745 at 1754.696 kb on - strand, within A4249_RS08745 at 1754.696 kb on - strand, within A4249_RS08745 at 1754.818 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 1,753,796 + -0.3 1,753,796 + -0.5 1,753,876 + +0.0 1,753,876 + +0.1 1,753,876 + -1.2 1,753,876 + -0.1 1,753,876 + -0.6 1,753,877 - +0.2 1,753,877 - +0.2 1,753,877 - -0.1 1,753,877 - -0.5 1,753,877 - -0.1 1,753,978 + A4249_RS08740 0.25 -0.1 1,753,978 + A4249_RS08740 0.25 -0.5 1,753,979 - A4249_RS08740 0.25 +0.2 1,753,979 - A4249_RS08740 0.25 +0.1 1,753,979 - A4249_RS08740 0.25 +0.2 1,754,040 - A4249_RS08740 0.38 +0.0 1,754,083 + A4249_RS08740 0.47 -0.5 1,754,083 + A4249_RS08740 0.47 -0.2 1,754,083 + A4249_RS08740 0.47 -0.2 1,754,083 + A4249_RS08740 0.47 -0.1 1,754,084 - A4249_RS08740 0.47 -0.3 1,754,084 - A4249_RS08740 0.47 -0.2 1,754,084 - A4249_RS08740 0.47 +0.3 1,754,084 - A4249_RS08740 0.47 -0.6 1,754,084 - A4249_RS08740 0.47 -0.3 1,754,084 - A4249_RS08740 0.47 +0.8 1,754,132 + A4249_RS08740 0.57 +0.3 1,754,132 + A4249_RS08740 0.57 +0.6 1,754,132 + A4249_RS08740 0.57 +0.6 1,754,133 - A4249_RS08740 0.58 -0.1 1,754,333 + -0.1 1,754,333 + +0.4 1,754,333 + -0.2 1,754,333 + -1.0 1,754,333 + +0.5 1,754,333 + -1.5 1,754,334 - +0.3 1,754,334 - -0.2 1,754,334 - -0.2 1,754,334 - +0.0 1,754,334 - -0.7 1,754,334 - -0.3 1,754,334 - -0.0 1,754,432 + -0.0 1,754,695 + A4249_RS08745 0.73 -1.3 1,754,695 + A4249_RS08745 0.73 -0.4 1,754,696 - A4249_RS08745 0.73 +0.7 1,754,696 - A4249_RS08745 0.73 -0.2 1,754,696 - A4249_RS08745 0.73 -0.3 1,754,696 - A4249_RS08745 0.73 -0.2 1,754,818 - -0.5
Or see this region's nucleotide sequence