Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS06645 and A4249_RS06650 are separated by 61 nucleotides A4249_RS06650 and A4249_RS06655 overlap by 4 nucleotides A4249_RS06655 and A4249_RS06660 overlap by 1 nucleotides A4249_RS06660 and A4249_RS06665 are separated by 72 nucleotides
A4249_RS06645: A4249_RS06645 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, at 1,354,316 to 1,355,239
_RS06645
A4249_RS06650: A4249_RS06650 - LemA family protein, at 1,355,301 to 1,355,984
_RS06650
A4249_RS06655: A4249_RS06655 - TPM domain-containing protein, at 1,355,981 to 1,356,808
_RS06655
A4249_RS06660: A4249_RS06660 - TPM domain-containing protein, at 1,356,808 to 1,357,485
_RS06660
A4249_RS06665: A4249_RS06665 - endonuclease/exonuclease/phosphatase family protein, at 1,357,558 to 1,358,295
_RS06665
Position (kb)
1355
1356
1357 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1355.213 kb on - strand at 1355.261 kb on + strand at 1355.261 kb on + strand at 1355.262 kb on - strand at 1355.262 kb on - strand at 1355.262 kb on - strand at 1355.262 kb on - strand at 1355.262 kb on - strand at 1355.262 kb on - strand at 1355.377 kb on - strand, within A4249_RS06650 at 1355.439 kb on + strand, within A4249_RS06650 at 1355.439 kb on + strand, within A4249_RS06650 at 1355.440 kb on - strand, within A4249_RS06650 at 1355.440 kb on - strand, within A4249_RS06650 at 1355.440 kb on - strand, within A4249_RS06650 at 1355.579 kb on + strand, within A4249_RS06650 at 1355.601 kb on + strand, within A4249_RS06650 at 1355.602 kb on - strand, within A4249_RS06650 at 1355.665 kb on - strand, within A4249_RS06650 at 1355.674 kb on + strand, within A4249_RS06650 at 1355.674 kb on + strand, within A4249_RS06650 at 1355.675 kb on - strand, within A4249_RS06650 at 1355.675 kb on - strand, within A4249_RS06650 at 1355.675 kb on - strand, within A4249_RS06650 at 1355.675 kb on - strand, within A4249_RS06650 at 1355.675 kb on - strand, within A4249_RS06650 at 1355.675 kb on - strand, within A4249_RS06650 at 1355.754 kb on + strand, within A4249_RS06650 at 1355.754 kb on + strand, within A4249_RS06650 at 1355.754 kb on + strand, within A4249_RS06650 at 1355.754 kb on + strand, within A4249_RS06650 at 1355.755 kb on - strand, within A4249_RS06650 at 1355.775 kb on + strand, within A4249_RS06650 at 1355.775 kb on + strand, within A4249_RS06650 at 1355.776 kb on - strand, within A4249_RS06650 at 1355.776 kb on - strand, within A4249_RS06650 at 1355.776 kb on - strand, within A4249_RS06650 at 1355.776 kb on - strand, within A4249_RS06650 at 1355.776 kb on - strand, within A4249_RS06650 at 1355.904 kb on - strand, within A4249_RS06650 at 1355.904 kb on - strand, within A4249_RS06650 at 1356.254 kb on + strand, within A4249_RS06655 at 1356.254 kb on + strand, within A4249_RS06655 at 1356.254 kb on + strand, within A4249_RS06655 at 1356.255 kb on - strand, within A4249_RS06655 at 1356.255 kb on - strand, within A4249_RS06655 at 1356.255 kb on - strand, within A4249_RS06655 at 1356.255 kb on - strand, within A4249_RS06655 at 1356.255 kb on - strand, within A4249_RS06655 at 1356.255 kb on - strand, within A4249_RS06655 at 1356.362 kb on + strand, within A4249_RS06655 at 1356.446 kb on + strand, within A4249_RS06655 at 1356.447 kb on - strand, within A4249_RS06655 at 1356.447 kb on - strand, within A4249_RS06655 at 1356.603 kb on + strand, within A4249_RS06655 at 1356.604 kb on - strand, within A4249_RS06655 at 1356.604 kb on - strand, within A4249_RS06655 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.916 kb on + strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1356.917 kb on - strand, within A4249_RS06660 at 1357.072 kb on + strand, within A4249_RS06660 at 1357.269 kb on + strand, within A4249_RS06660 at 1357.269 kb on + strand, within A4249_RS06660 at 1357.269 kb on + strand, within A4249_RS06660 at 1357.269 kb on + strand, within A4249_RS06660 at 1357.269 kb on + strand, within A4249_RS06660 at 1357.269 kb on + strand, within A4249_RS06660 at 1357.269 kb on + strand, within A4249_RS06660 at 1357.269 kb on + strand, within A4249_RS06660 at 1357.269 kb on + strand, within A4249_RS06660 at 1357.269 kb on + strand, within A4249_RS06660 at 1357.270 kb on - strand, within A4249_RS06660 at 1357.270 kb on - strand, within A4249_RS06660 at 1357.270 kb on - strand, within A4249_RS06660 at 1357.270 kb on - strand, within A4249_RS06660 at 1357.270 kb on - strand, within A4249_RS06660 at 1357.270 kb on - strand, within A4249_RS06660 at 1357.270 kb on - strand, within A4249_RS06660 at 1357.270 kb on - strand, within A4249_RS06660 at 1357.300 kb on + strand, within A4249_RS06660 at 1357.300 kb on + strand, within A4249_RS06660 at 1357.301 kb on - strand, within A4249_RS06660 at 1357.301 kb on - strand, within A4249_RS06660 at 1357.572 kb on + strand at 1357.572 kb on + strand at 1357.572 kb on + strand at 1357.572 kb on + strand at 1357.572 kb on + strand at 1357.572 kb on + strand at 1357.572 kb on + strand at 1357.573 kb on - strand at 1357.576 kb on + strand at 1357.576 kb on + strand at 1357.576 kb on + strand at 1357.576 kb on + strand at 1357.576 kb on + strand at 1357.576 kb on + strand at 1357.576 kb on + strand at 1357.577 kb on - strand at 1357.577 kb on - strand at 1357.577 kb on - strand at 1357.577 kb on - strand at 1357.789 kb on + strand, within A4249_RS06665
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 1,355,213 - -1.4 1,355,261 + +0.6 1,355,261 + +0.9 1,355,262 - +1.6 1,355,262 - +0.4 1,355,262 - +0.2 1,355,262 - -0.3 1,355,262 - -0.4 1,355,262 - +0.1 1,355,377 - A4249_RS06650 0.11 -0.9 1,355,439 + A4249_RS06650 0.20 +0.2 1,355,439 + A4249_RS06650 0.20 +0.0 1,355,440 - A4249_RS06650 0.20 -0.5 1,355,440 - A4249_RS06650 0.20 -0.6 1,355,440 - A4249_RS06650 0.20 +1.1 1,355,579 + A4249_RS06650 0.41 -0.4 1,355,601 + A4249_RS06650 0.44 -0.2 1,355,602 - A4249_RS06650 0.44 -0.5 1,355,665 - A4249_RS06650 0.53 +0.1 1,355,674 + A4249_RS06650 0.55 -0.5 1,355,674 + A4249_RS06650 0.55 -0.5 1,355,675 - A4249_RS06650 0.55 +0.2 1,355,675 - A4249_RS06650 0.55 -0.7 1,355,675 - A4249_RS06650 0.55 +0.8 1,355,675 - A4249_RS06650 0.55 +1.3 1,355,675 - A4249_RS06650 0.55 -0.2 1,355,675 - A4249_RS06650 0.55 +0.5 1,355,754 + A4249_RS06650 0.66 -0.2 1,355,754 + A4249_RS06650 0.66 +0.0 1,355,754 + A4249_RS06650 0.66 +1.6 1,355,754 + A4249_RS06650 0.66 +0.3 1,355,755 - A4249_RS06650 0.66 -0.4 1,355,775 + A4249_RS06650 0.69 -0.5 1,355,775 + A4249_RS06650 0.69 -0.6 1,355,776 - A4249_RS06650 0.69 -1.1 1,355,776 - A4249_RS06650 0.69 +0.1 1,355,776 - A4249_RS06650 0.69 -0.8 1,355,776 - A4249_RS06650 0.69 -0.1 1,355,776 - A4249_RS06650 0.69 -0.5 1,355,904 - A4249_RS06650 0.88 -0.5 1,355,904 - A4249_RS06650 0.88 -0.0 1,356,254 + A4249_RS06655 0.33 +0.4 1,356,254 + A4249_RS06655 0.33 +0.1 1,356,254 + A4249_RS06655 0.33 -0.7 1,356,255 - A4249_RS06655 0.33 -0.4 1,356,255 - A4249_RS06655 0.33 +0.4 1,356,255 - A4249_RS06655 0.33 -0.6 1,356,255 - A4249_RS06655 0.33 +1.0 1,356,255 - A4249_RS06655 0.33 +0.3 1,356,255 - A4249_RS06655 0.33 -0.9 1,356,362 + A4249_RS06655 0.46 -0.4 1,356,446 + A4249_RS06655 0.56 +0.5 1,356,447 - A4249_RS06655 0.56 +0.7 1,356,447 - A4249_RS06655 0.56 -0.3 1,356,603 + A4249_RS06655 0.75 -0.3 1,356,604 - A4249_RS06655 0.75 -0.9 1,356,604 - A4249_RS06655 0.75 +0.0 1,356,916 + A4249_RS06660 0.16 +0.1 1,356,916 + A4249_RS06660 0.16 -0.0 1,356,916 + A4249_RS06660 0.16 +0.5 1,356,916 + A4249_RS06660 0.16 -0.4 1,356,916 + A4249_RS06660 0.16 +0.1 1,356,916 + A4249_RS06660 0.16 +0.4 1,356,916 + A4249_RS06660 0.16 -0.2 1,356,916 + A4249_RS06660 0.16 +0.9 1,356,916 + A4249_RS06660 0.16 +0.4 1,356,916 + A4249_RS06660 0.16 +0.0 1,356,916 + A4249_RS06660 0.16 +0.8 1,356,916 + A4249_RS06660 0.16 -0.1 1,356,916 + A4249_RS06660 0.16 +0.5 1,356,916 + A4249_RS06660 0.16 -0.2 1,356,916 + A4249_RS06660 0.16 -1.1 1,356,916 + A4249_RS06660 0.16 +1.4 1,356,917 - A4249_RS06660 0.16 +0.5 1,356,917 - A4249_RS06660 0.16 +0.2 1,356,917 - A4249_RS06660 0.16 +0.4 1,356,917 - A4249_RS06660 0.16 -0.4 1,356,917 - A4249_RS06660 0.16 +0.2 1,356,917 - A4249_RS06660 0.16 +0.4 1,356,917 - A4249_RS06660 0.16 -0.7 1,356,917 - A4249_RS06660 0.16 -2.2 1,356,917 - A4249_RS06660 0.16 -0.1 1,356,917 - A4249_RS06660 0.16 -0.4 1,356,917 - A4249_RS06660 0.16 -1.0 1,356,917 - A4249_RS06660 0.16 +1.9 1,356,917 - A4249_RS06660 0.16 -0.1 1,356,917 - A4249_RS06660 0.16 +1.0 1,357,072 + A4249_RS06660 0.39 +0.3 1,357,269 + A4249_RS06660 0.68 +0.8 1,357,269 + A4249_RS06660 0.68 +0.3 1,357,269 + A4249_RS06660 0.68 +1.1 1,357,269 + A4249_RS06660 0.68 -0.9 1,357,269 + A4249_RS06660 0.68 -1.1 1,357,269 + A4249_RS06660 0.68 -0.1 1,357,269 + A4249_RS06660 0.68 -1.2 1,357,269 + A4249_RS06660 0.68 +0.3 1,357,269 + A4249_RS06660 0.68 -1.4 1,357,269 + A4249_RS06660 0.68 +0.4 1,357,270 - A4249_RS06660 0.68 -0.2 1,357,270 - A4249_RS06660 0.68 -0.3 1,357,270 - A4249_RS06660 0.68 -0.8 1,357,270 - A4249_RS06660 0.68 -0.5 1,357,270 - A4249_RS06660 0.68 +0.5 1,357,270 - A4249_RS06660 0.68 -0.0 1,357,270 - A4249_RS06660 0.68 +0.2 1,357,270 - A4249_RS06660 0.68 +0.7 1,357,300 + A4249_RS06660 0.73 -0.6 1,357,300 + A4249_RS06660 0.73 +0.7 1,357,301 - A4249_RS06660 0.73 -1.6 1,357,301 - A4249_RS06660 0.73 -0.2 1,357,572 + -1.2 1,357,572 + +1.6 1,357,572 + -0.3 1,357,572 + +1.6 1,357,572 + +0.4 1,357,572 + -0.1 1,357,572 + -1.7 1,357,573 - +0.8 1,357,576 + +0.4 1,357,576 + -0.2 1,357,576 + -1.2 1,357,576 + +0.6 1,357,576 + -1.7 1,357,576 + -0.4 1,357,576 + +0.9 1,357,577 - +0.5 1,357,577 - -0.7 1,357,577 - +0.4 1,357,577 - +1.6 1,357,789 + A4249_RS06665 0.31 +0.3
Or see this region's nucleotide sequence