Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS03670 and A4249_RS03675 are separated by 7 nucleotides A4249_RS03675 and A4249_RS03680 are separated by 175 nucleotides A4249_RS03680 and A4249_RS03685 are separated by 24 nucleotides A4249_RS03685 and A4249_RS03690 overlap by 11 nucleotides A4249_RS03690 and A4249_RS03695 are separated by 69 nucleotides
A4249_RS03670: A4249_RS03670 - endonuclease/exonuclease/phosphatase family protein, at 776,089 to 776,898
_RS03670
A4249_RS03675: A4249_RS03675 - DUF2188 domain-containing protein, at 776,906 to 777,145
_RS03675
A4249_RS03680: A4249_RS03680 - DUF6249 domain-containing protein, at 777,321 to 777,719
_RS03680
A4249_RS03685: A4249_RS03685 - sigma-70 family RNA polymerase sigma factor, at 777,744 to 778,301
_RS03685
A4249_RS03690: A4249_RS03690 - hypothetical protein, at 778,291 to 778,707
_RS03690
A4249_RS03695: A4249_RS03695 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 778,777 to 779,613
_RS03695
Position (kb) 777
778
779 Strain fitness (log2 ratio) -1
0
1 at 776.823 kb on + strand at 776.824 kb on - strand at 776.824 kb on - strand at 776.982 kb on + strand, within A4249_RS03675 at 776.983 kb on - strand, within A4249_RS03675 at 777.078 kb on + strand, within A4249_RS03675 at 777.078 kb on + strand, within A4249_RS03675 at 777.078 kb on + strand, within A4249_RS03675 at 777.079 kb on - strand, within A4249_RS03675 at 777.079 kb on - strand, within A4249_RS03675 at 777.079 kb on - strand, within A4249_RS03675 at 777.079 kb on - strand, within A4249_RS03675 at 777.096 kb on + strand, within A4249_RS03675 at 777.097 kb on - strand, within A4249_RS03675 at 777.097 kb on - strand, within A4249_RS03675 at 777.097 kb on - strand, within A4249_RS03675 at 777.129 kb on + strand at 777.129 kb on + strand at 777.129 kb on + strand at 777.130 kb on - strand at 777.180 kb on + strand at 777.181 kb on - strand at 777.181 kb on - strand at 777.214 kb on + strand at 777.214 kb on + strand at 777.215 kb on - strand at 777.215 kb on - strand at 777.215 kb on - strand at 777.215 kb on - strand at 777.229 kb on + strand at 777.230 kb on - strand at 777.313 kb on + strand at 777.314 kb on - strand at 777.314 kb on - strand at 777.371 kb on + strand, within A4249_RS03680 at 777.371 kb on + strand, within A4249_RS03680 at 777.372 kb on - strand, within A4249_RS03680 at 777.405 kb on + strand, within A4249_RS03680 at 777.405 kb on + strand, within A4249_RS03680 at 777.406 kb on - strand, within A4249_RS03680 at 777.605 kb on + strand, within A4249_RS03680 at 777.606 kb on - strand, within A4249_RS03680 at 777.606 kb on - strand, within A4249_RS03680 at 777.606 kb on - strand, within A4249_RS03680 at 777.606 kb on - strand, within A4249_RS03680 at 777.656 kb on + strand, within A4249_RS03680 at 777.657 kb on - strand, within A4249_RS03680 at 777.657 kb on - strand, within A4249_RS03680 at 777.816 kb on + strand, within A4249_RS03685 at 777.816 kb on + strand, within A4249_RS03685 at 777.816 kb on + strand, within A4249_RS03685 at 777.816 kb on + strand, within A4249_RS03685 at 777.816 kb on + strand, within A4249_RS03685 at 777.817 kb on - strand, within A4249_RS03685 at 777.817 kb on - strand, within A4249_RS03685 at 777.817 kb on - strand, within A4249_RS03685 at 777.817 kb on - strand, within A4249_RS03685 at 777.999 kb on + strand, within A4249_RS03685 at 778.000 kb on - strand, within A4249_RS03685 at 778.029 kb on + strand, within A4249_RS03685 at 778.029 kb on + strand, within A4249_RS03685 at 778.029 kb on + strand, within A4249_RS03685 at 778.029 kb on + strand, within A4249_RS03685 at 778.029 kb on + strand, within A4249_RS03685 at 778.030 kb on - strand, within A4249_RS03685 at 778.030 kb on - strand, within A4249_RS03685 at 778.030 kb on - strand, within A4249_RS03685 at 778.030 kb on - strand, within A4249_RS03685 at 778.030 kb on - strand, within A4249_RS03685 at 778.239 kb on - strand, within A4249_RS03685 at 778.500 kb on + strand, within A4249_RS03690 at 778.501 kb on - strand, within A4249_RS03690 at 778.745 kb on + strand at 778.745 kb on + strand at 778.745 kb on + strand at 778.745 kb on + strand at 778.745 kb on + strand at 778.746 kb on - strand at 778.746 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 776,823 + -1.0 776,824 - +0.1 776,824 - -0.8 776,982 + A4249_RS03675 0.32 +0.2 776,983 - A4249_RS03675 0.32 +0.3 777,078 + A4249_RS03675 0.72 -1.0 777,078 + A4249_RS03675 0.72 +0.7 777,078 + A4249_RS03675 0.72 +0.4 777,079 - A4249_RS03675 0.72 -0.1 777,079 - A4249_RS03675 0.72 -0.2 777,079 - A4249_RS03675 0.72 +0.2 777,079 - A4249_RS03675 0.72 -0.8 777,096 + A4249_RS03675 0.79 -0.1 777,097 - A4249_RS03675 0.80 -0.2 777,097 - A4249_RS03675 0.80 -0.8 777,097 - A4249_RS03675 0.80 +0.4 777,129 + -1.0 777,129 + -0.3 777,129 + +0.4 777,130 - -0.1 777,180 + -0.9 777,181 - +0.6 777,181 - -0.2 777,214 + -0.2 777,214 + -0.6 777,215 - -0.3 777,215 - +0.5 777,215 - -0.5 777,215 - -0.1 777,229 + -0.6 777,230 - -0.0 777,313 + +0.8 777,314 - -0.2 777,314 - +1.3 777,371 + A4249_RS03680 0.13 -0.7 777,371 + A4249_RS03680 0.13 +0.5 777,372 - A4249_RS03680 0.13 +0.1 777,405 + A4249_RS03680 0.21 +0.5 777,405 + A4249_RS03680 0.21 +0.0 777,406 - A4249_RS03680 0.21 -0.7 777,605 + A4249_RS03680 0.71 -0.4 777,606 - A4249_RS03680 0.71 -0.1 777,606 - A4249_RS03680 0.71 +0.4 777,606 - A4249_RS03680 0.71 -0.2 777,606 - A4249_RS03680 0.71 +0.3 777,656 + A4249_RS03680 0.84 -0.6 777,657 - A4249_RS03680 0.84 -1.6 777,657 - A4249_RS03680 0.84 -0.1 777,816 + A4249_RS03685 0.13 -0.6 777,816 + A4249_RS03685 0.13 -0.2 777,816 + A4249_RS03685 0.13 +0.1 777,816 + A4249_RS03685 0.13 -1.0 777,816 + A4249_RS03685 0.13 -0.4 777,817 - A4249_RS03685 0.13 -1.4 777,817 - A4249_RS03685 0.13 -0.5 777,817 - A4249_RS03685 0.13 +0.5 777,817 - A4249_RS03685 0.13 +0.4 777,999 + A4249_RS03685 0.46 +0.4 778,000 - A4249_RS03685 0.46 -0.4 778,029 + A4249_RS03685 0.51 +0.0 778,029 + A4249_RS03685 0.51 +0.3 778,029 + A4249_RS03685 0.51 +0.3 778,029 + A4249_RS03685 0.51 -0.0 778,029 + A4249_RS03685 0.51 +0.4 778,030 - A4249_RS03685 0.51 +0.2 778,030 - A4249_RS03685 0.51 -0.8 778,030 - A4249_RS03685 0.51 -1.0 778,030 - A4249_RS03685 0.51 -0.0 778,030 - A4249_RS03685 0.51 -0.1 778,239 - A4249_RS03685 0.89 +0.6 778,500 + A4249_RS03690 0.50 +0.3 778,501 - A4249_RS03690 0.50 +0.2 778,745 + +0.0 778,745 + +0.3 778,745 + -0.0 778,745 + +0.0 778,745 + -0.3 778,746 - -0.1 778,746 - +1.1
Or see this region's nucleotide sequence