Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS03035 and A4249_RS03040 overlap by 4 nucleotides A4249_RS03040 and A4249_RS03045 overlap by 4 nucleotides A4249_RS03045 and A4249_RS03050 are separated by 281 nucleotides
A4249_RS03035: A4249_RS03035 - RsmB/NOP family class I SAM-dependent RNA methyltransferase, at 642,213 to 643,514
_RS03035
A4249_RS03040: A4249_RS03040 - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 643,511 to 644,224
_RS03040
A4249_RS03045: A4249_RS03045 - glutamine-hydrolyzing GMP synthase, at 644,221 to 645,783
_RS03045
A4249_RS03050: A4249_RS03050 - tyrosine-type recombinase/integrase, at 646,065 to 647,311
_RS03050
Position (kb) 644
645
646 Strain fitness (log2 ratio) -2
-1
0
1 at 643.232 kb on + strand, within A4249_RS03035 at 643.232 kb on + strand, within A4249_RS03035 at 643.232 kb on + strand, within A4249_RS03035 at 643.232 kb on + strand, within A4249_RS03035 at 643.233 kb on - strand, within A4249_RS03035 at 643.233 kb on - strand, within A4249_RS03035 at 643.233 kb on - strand, within A4249_RS03035 at 643.233 kb on - strand, within A4249_RS03035 at 643.233 kb on - strand, within A4249_RS03035 at 643.233 kb on - strand, within A4249_RS03035 at 643.281 kb on + strand, within A4249_RS03035 at 643.281 kb on + strand, within A4249_RS03035 at 643.281 kb on + strand, within A4249_RS03035 at 643.281 kb on + strand, within A4249_RS03035 at 643.281 kb on + strand, within A4249_RS03035 at 643.281 kb on + strand, within A4249_RS03035 at 643.282 kb on - strand, within A4249_RS03035 at 643.282 kb on - strand, within A4249_RS03035 at 643.282 kb on - strand, within A4249_RS03035 at 643.282 kb on - strand, within A4249_RS03035 at 643.282 kb on - strand, within A4249_RS03035 at 643.282 kb on - strand, within A4249_RS03035 at 643.282 kb on - strand, within A4249_RS03035 at 643.282 kb on - strand, within A4249_RS03035 at 643.498 kb on - strand at 643.498 kb on - strand at 643.547 kb on + strand at 643.547 kb on + strand at 643.547 kb on + strand at 643.547 kb on + strand at 643.547 kb on + strand at 643.547 kb on + strand at 643.547 kb on + strand at 643.547 kb on + strand at 643.547 kb on + strand at 643.548 kb on - strand at 643.548 kb on - strand at 643.548 kb on - strand at 643.548 kb on - strand at 643.548 kb on - strand at 643.548 kb on - strand at 643.548 kb on - strand at 643.548 kb on - strand at 643.548 kb on - strand at 643.548 kb on - strand at 643.548 kb on - strand at 643.548 kb on - strand at 643.548 kb on - strand at 643.572 kb on - strand at 643.698 kb on + strand, within A4249_RS03040 at 643.699 kb on - strand, within A4249_RS03040 at 643.745 kb on + strand, within A4249_RS03040 at 643.745 kb on + strand, within A4249_RS03040 at 643.746 kb on - strand, within A4249_RS03040 at 643.746 kb on - strand, within A4249_RS03040 at 643.746 kb on - strand, within A4249_RS03040 at 643.916 kb on + strand, within A4249_RS03040 at 643.916 kb on + strand, within A4249_RS03040 at 643.917 kb on - strand, within A4249_RS03040 at 643.917 kb on - strand, within A4249_RS03040 at 643.917 kb on - strand, within A4249_RS03040 at 643.917 kb on - strand, within A4249_RS03040 at 643.917 kb on - strand, within A4249_RS03040 at 644.004 kb on + strand, within A4249_RS03040 at 644.163 kb on + strand at 645.846 kb on + strand at 645.876 kb on + strand at 645.952 kb on - strand at 646.140 kb on + strand at 646.140 kb on + strand at 646.141 kb on - strand at 646.185 kb on + strand at 646.227 kb on + strand, within A4249_RS03050 at 646.227 kb on + strand, within A4249_RS03050 at 646.228 kb on - strand, within A4249_RS03050 at 646.571 kb on + strand, within A4249_RS03050 at 646.571 kb on + strand, within A4249_RS03050 at 646.572 kb on - strand, within A4249_RS03050 at 646.572 kb on - strand, within A4249_RS03050 at 646.572 kb on - strand, within A4249_RS03050 at 646.577 kb on + strand, within A4249_RS03050 at 646.577 kb on + strand, within A4249_RS03050 at 646.577 kb on + strand, within A4249_RS03050 at 646.578 kb on - strand, within A4249_RS03050
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 643,232 + A4249_RS03035 0.78 +1.4 643,232 + A4249_RS03035 0.78 +0.2 643,232 + A4249_RS03035 0.78 -2.5 643,232 + A4249_RS03035 0.78 -0.2 643,233 - A4249_RS03035 0.78 +0.2 643,233 - A4249_RS03035 0.78 +1.1 643,233 - A4249_RS03035 0.78 +0.0 643,233 - A4249_RS03035 0.78 +0.4 643,233 - A4249_RS03035 0.78 +1.1 643,233 - A4249_RS03035 0.78 +0.2 643,281 + A4249_RS03035 0.82 -0.0 643,281 + A4249_RS03035 0.82 -0.0 643,281 + A4249_RS03035 0.82 +1.0 643,281 + A4249_RS03035 0.82 +0.0 643,281 + A4249_RS03035 0.82 +0.5 643,281 + A4249_RS03035 0.82 -0.0 643,282 - A4249_RS03035 0.82 +0.0 643,282 - A4249_RS03035 0.82 -0.3 643,282 - A4249_RS03035 0.82 +0.2 643,282 - A4249_RS03035 0.82 +0.3 643,282 - A4249_RS03035 0.82 +1.0 643,282 - A4249_RS03035 0.82 -0.3 643,282 - A4249_RS03035 0.82 -0.4 643,282 - A4249_RS03035 0.82 -1.3 643,498 - -0.7 643,498 - +0.0 643,547 + +0.2 643,547 + -0.8 643,547 + -0.3 643,547 + +0.1 643,547 + +0.3 643,547 + -0.8 643,547 + +1.5 643,547 + +0.3 643,547 + +0.2 643,548 - +0.2 643,548 - +0.1 643,548 - +0.1 643,548 - -0.4 643,548 - -0.2 643,548 - -0.3 643,548 - -0.9 643,548 - -0.6 643,548 - +0.0 643,548 - +0.1 643,548 - +0.1 643,548 - +0.4 643,548 - +0.5 643,572 - -0.6 643,698 + A4249_RS03040 0.26 +0.2 643,699 - A4249_RS03040 0.26 -0.4 643,745 + A4249_RS03040 0.33 -1.0 643,745 + A4249_RS03040 0.33 -1.2 643,746 - A4249_RS03040 0.33 -2.5 643,746 - A4249_RS03040 0.33 -0.4 643,746 - A4249_RS03040 0.33 -0.0 643,916 + A4249_RS03040 0.57 +0.4 643,916 + A4249_RS03040 0.57 +0.4 643,917 - A4249_RS03040 0.57 +0.4 643,917 - A4249_RS03040 0.57 -0.8 643,917 - A4249_RS03040 0.57 -0.5 643,917 - A4249_RS03040 0.57 +0.8 643,917 - A4249_RS03040 0.57 +0.2 644,004 + A4249_RS03040 0.69 -1.4 644,163 + +0.4 645,846 + -0.3 645,876 + -0.1 645,952 - -0.8 646,140 + +0.3 646,140 + +0.1 646,141 - +0.5 646,185 + -1.3 646,227 + A4249_RS03050 0.13 +0.3 646,227 + A4249_RS03050 0.13 +0.2 646,228 - A4249_RS03050 0.13 -0.6 646,571 + A4249_RS03050 0.41 -1.0 646,571 + A4249_RS03050 0.41 +0.5 646,572 - A4249_RS03050 0.41 +0.9 646,572 - A4249_RS03050 0.41 -0.4 646,572 - A4249_RS03050 0.41 +0.2 646,577 + A4249_RS03050 0.41 -0.8 646,577 + A4249_RS03050 0.41 +0.0 646,577 + A4249_RS03050 0.41 +1.1 646,578 - A4249_RS03050 0.41 -1.4
Or see this region's nucleotide sequence