Experiment: Adenine 0.74 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS02455 and A4249_RS16480 are separated by 5 nucleotides A4249_RS16480 and A4249_RS02465 are separated by 35 nucleotides A4249_RS02465 and A4249_RS02475 are separated by 60 nucleotides
A4249_RS02455: A4249_RS02455 - acyl-CoA carboxylase subunit beta, at 527,247 to 528,782
_RS02455
A4249_RS16480: A4249_RS16480 - hypothetical protein, at 528,788 to 529,579
_RS16480
A4249_RS02465: A4249_RS02465 - acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha, at 529,615 to 531,357
_RS02465
A4249_RS02475: A4249_RS02475 - IS3 family transposase, at 531,418 to 532,607
_RS02475
Position (kb) 529
530
531
532 Strain fitness (log2 ratio) -2
-1
0
1 at 528.618 kb on + strand, within A4249_RS02455 at 528.618 kb on + strand, within A4249_RS02455 at 528.619 kb on - strand, within A4249_RS02455 at 528.663 kb on + strand at 528.663 kb on + strand at 528.663 kb on + strand at 528.664 kb on - strand at 528.664 kb on - strand at 528.666 kb on - strand at 528.770 kb on + strand at 528.770 kb on + strand at 528.771 kb on - strand at 528.771 kb on - strand at 528.783 kb on + strand at 528.784 kb on - strand at 528.785 kb on + strand at 528.785 kb on + strand at 528.785 kb on + strand at 528.785 kb on + strand at 528.785 kb on + strand at 528.785 kb on + strand at 528.785 kb on + strand at 528.785 kb on + strand at 528.785 kb on + strand at 528.785 kb on + strand at 528.785 kb on + strand at 528.786 kb on - strand at 528.786 kb on - strand at 528.786 kb on - strand at 528.786 kb on - strand at 528.786 kb on - strand at 528.786 kb on - strand at 528.786 kb on - strand at 528.786 kb on - strand at 528.786 kb on - strand at 528.786 kb on - strand at 528.786 kb on - strand at 528.786 kb on - strand at 528.786 kb on - strand at 528.834 kb on + strand at 528.834 kb on + strand at 528.835 kb on - strand at 528.860 kb on + strand at 528.861 kb on - strand at 528.861 kb on - strand at 528.904 kb on + strand, within A4249_RS16480 at 528.904 kb on + strand, within A4249_RS16480 at 528.904 kb on + strand, within A4249_RS16480 at 528.905 kb on - strand, within A4249_RS16480 at 528.923 kb on - strand, within A4249_RS16480 at 528.953 kb on + strand, within A4249_RS16480 at 528.953 kb on + strand, within A4249_RS16480 at 528.953 kb on + strand, within A4249_RS16480 at 528.953 kb on + strand, within A4249_RS16480 at 528.953 kb on + strand, within A4249_RS16480 at 528.953 kb on + strand, within A4249_RS16480 at 528.953 kb on + strand, within A4249_RS16480 at 528.953 kb on + strand, within A4249_RS16480 at 528.953 kb on + strand, within A4249_RS16480 at 528.954 kb on - strand, within A4249_RS16480 at 528.954 kb on - strand, within A4249_RS16480 at 528.954 kb on - strand, within A4249_RS16480 at 528.954 kb on - strand, within A4249_RS16480 at 528.954 kb on - strand, within A4249_RS16480 at 528.954 kb on - strand, within A4249_RS16480 at 528.954 kb on - strand, within A4249_RS16480 at 528.954 kb on - strand, within A4249_RS16480 at 528.954 kb on - strand, within A4249_RS16480 at 528.954 kb on - strand, within A4249_RS16480 at 528.964 kb on + strand, within A4249_RS16480 at 528.965 kb on - strand, within A4249_RS16480 at 528.965 kb on - strand, within A4249_RS16480 at 529.071 kb on + strand, within A4249_RS16480 at 529.071 kb on + strand, within A4249_RS16480 at 529.071 kb on + strand, within A4249_RS16480 at 529.071 kb on + strand, within A4249_RS16480 at 529.071 kb on + strand, within A4249_RS16480 at 529.072 kb on - strand, within A4249_RS16480 at 529.072 kb on - strand, within A4249_RS16480 at 529.072 kb on - strand, within A4249_RS16480 at 529.072 kb on - strand, within A4249_RS16480 at 529.072 kb on - strand, within A4249_RS16480 at 529.072 kb on - strand, within A4249_RS16480 at 529.072 kb on - strand, within A4249_RS16480 at 529.158 kb on + strand, within A4249_RS16480 at 529.158 kb on + strand, within A4249_RS16480 at 529.158 kb on + strand, within A4249_RS16480 at 529.158 kb on + strand, within A4249_RS16480 at 529.158 kb on + strand, within A4249_RS16480 at 529.159 kb on - strand, within A4249_RS16480 at 529.159 kb on - strand, within A4249_RS16480 at 529.159 kb on - strand, within A4249_RS16480 at 529.159 kb on - strand, within A4249_RS16480 at 529.159 kb on - strand, within A4249_RS16480 at 529.159 kb on - strand, within A4249_RS16480 at 529.177 kb on - strand, within A4249_RS16480 at 529.177 kb on - strand, within A4249_RS16480 at 529.177 kb on - strand, within A4249_RS16480 at 529.177 kb on - strand, within A4249_RS16480 at 529.192 kb on + strand, within A4249_RS16480 at 529.235 kb on + strand, within A4249_RS16480 at 529.235 kb on + strand, within A4249_RS16480 at 529.235 kb on + strand, within A4249_RS16480 at 529.236 kb on - strand, within A4249_RS16480 at 529.236 kb on - strand, within A4249_RS16480 at 529.271 kb on + strand, within A4249_RS16480 at 529.271 kb on + strand, within A4249_RS16480 at 529.271 kb on + strand, within A4249_RS16480 at 529.272 kb on - strand, within A4249_RS16480 at 529.272 kb on - strand, within A4249_RS16480 at 529.272 kb on - strand, within A4249_RS16480 at 529.273 kb on + strand, within A4249_RS16480 at 529.274 kb on - strand, within A4249_RS16480 at 529.274 kb on - strand, within A4249_RS16480 at 529.282 kb on + strand, within A4249_RS16480 at 529.282 kb on + strand, within A4249_RS16480 at 529.282 kb on + strand, within A4249_RS16480 at 529.283 kb on - strand, within A4249_RS16480 at 529.285 kb on + strand, within A4249_RS16480 at 529.383 kb on + strand, within A4249_RS16480 at 529.421 kb on + strand, within A4249_RS16480 at 529.421 kb on + strand, within A4249_RS16480 at 529.421 kb on + strand, within A4249_RS16480 at 529.422 kb on - strand, within A4249_RS16480 at 529.436 kb on - strand, within A4249_RS16480 at 529.513 kb on + strand at 529.521 kb on + strand at 529.522 kb on - strand at 529.522 kb on - strand at 529.577 kb on + strand at 529.578 kb on - strand at 529.578 kb on - strand at 529.578 kb on - strand at 529.591 kb on + strand at 529.591 kb on + strand at 529.592 kb on - strand at 529.592 kb on - strand at 529.595 kb on + strand at 529.595 kb on + strand at 529.595 kb on + strand at 529.595 kb on + strand at 529.596 kb on - strand at 529.596 kb on - strand at 529.708 kb on + strand at 529.792 kb on + strand, within A4249_RS02465 at 529.792 kb on + strand, within A4249_RS02465 at 529.792 kb on + strand, within A4249_RS02465 at 529.792 kb on + strand, within A4249_RS02465 at 529.792 kb on + strand, within A4249_RS02465 at 529.793 kb on - strand, within A4249_RS02465 at 529.793 kb on - strand, within A4249_RS02465 at 529.793 kb on - strand, within A4249_RS02465 at 529.793 kb on - strand, within A4249_RS02465 at 530.016 kb on + strand, within A4249_RS02465 at 530.016 kb on + strand, within A4249_RS02465 at 530.416 kb on + strand, within A4249_RS02465 at 530.416 kb on + strand, within A4249_RS02465 at 530.416 kb on + strand, within A4249_RS02465 at 530.416 kb on + strand, within A4249_RS02465 at 530.416 kb on + strand, within A4249_RS02465 at 530.417 kb on - strand, within A4249_RS02465 at 530.417 kb on - strand, within A4249_RS02465 at 530.417 kb on - strand, within A4249_RS02465 at 530.417 kb on - strand, within A4249_RS02465 at 530.417 kb on - strand, within A4249_RS02465 at 530.417 kb on - strand, within A4249_RS02465 at 530.417 kb on - strand, within A4249_RS02465 at 530.417 kb on - strand, within A4249_RS02465 at 530.632 kb on + strand, within A4249_RS02465 at 530.632 kb on + strand, within A4249_RS02465 at 530.633 kb on - strand, within A4249_RS02465 at 530.633 kb on - strand, within A4249_RS02465 at 530.633 kb on - strand, within A4249_RS02465 at 530.633 kb on - strand, within A4249_RS02465 at 530.687 kb on - strand, within A4249_RS02465 at 530.687 kb on - strand, within A4249_RS02465 at 530.719 kb on + strand, within A4249_RS02465 at 530.719 kb on + strand, within A4249_RS02465 at 530.719 kb on + strand, within A4249_RS02465 at 530.719 kb on + strand, within A4249_RS02465 at 530.719 kb on + strand, within A4249_RS02465 at 530.720 kb on - strand, within A4249_RS02465 at 530.720 kb on - strand, within A4249_RS02465 at 530.720 kb on - strand, within A4249_RS02465 at 530.720 kb on - strand, within A4249_RS02465 at 530.720 kb on - strand, within A4249_RS02465 at 530.720 kb on - strand, within A4249_RS02465 at 530.720 kb on - strand, within A4249_RS02465 at 530.720 kb on - strand, within A4249_RS02465 at 530.773 kb on + strand, within A4249_RS02465 at 530.773 kb on + strand, within A4249_RS02465 at 530.773 kb on + strand, within A4249_RS02465 at 530.773 kb on + strand, within A4249_RS02465 at 530.773 kb on + strand, within A4249_RS02465 at 530.773 kb on + strand, within A4249_RS02465 at 530.773 kb on + strand, within A4249_RS02465 at 530.773 kb on + strand, within A4249_RS02465 at 530.774 kb on - strand, within A4249_RS02465 at 530.774 kb on - strand, within A4249_RS02465 at 530.774 kb on - strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.947 kb on + strand, within A4249_RS02465 at 530.948 kb on - strand, within A4249_RS02465 at 530.948 kb on - strand, within A4249_RS02465 at 530.948 kb on - strand, within A4249_RS02465 at 530.948 kb on - strand, within A4249_RS02465 at 530.948 kb on - strand, within A4249_RS02465 at 530.948 kb on - strand, within A4249_RS02465 at 530.948 kb on - strand, within A4249_RS02465 at 530.949 kb on + strand, within A4249_RS02465 at 530.949 kb on + strand, within A4249_RS02465 at 530.949 kb on + strand, within A4249_RS02465 at 530.949 kb on + strand, within A4249_RS02465 at 530.949 kb on + strand, within A4249_RS02465 at 530.949 kb on + strand, within A4249_RS02465 at 530.949 kb on + strand, within A4249_RS02465 at 530.950 kb on - strand, within A4249_RS02465 at 530.950 kb on - strand, within A4249_RS02465 at 531.130 kb on + strand, within A4249_RS02465 at 531.130 kb on + strand, within A4249_RS02465 at 531.131 kb on - strand, within A4249_RS02465 at 531.346 kb on + strand at 531.346 kb on + strand at 531.347 kb on - strand at 531.347 kb on - strand at 531.347 kb on - strand at 531.347 kb on - strand at 531.386 kb on - strand at 531.386 kb on - strand at 531.386 kb on - strand at 531.386 kb on - strand at 531.390 kb on - strand at 531.390 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine 0.74 mM (C) remove 528,618 + A4249_RS02455 0.89 -1.7 528,618 + A4249_RS02455 0.89 +0.6 528,619 - A4249_RS02455 0.89 +0.1 528,663 + -0.1 528,663 + -0.2 528,663 + -0.3 528,664 - -0.7 528,664 - +0.1 528,666 - +0.5 528,770 + -0.5 528,770 + +0.6 528,771 - -0.2 528,771 - -1.7 528,783 + +0.1 528,784 - -0.8 528,785 + -0.0 528,785 + +0.7 528,785 + +0.3 528,785 + +0.6 528,785 + +0.6 528,785 + -0.1 528,785 + -0.7 528,785 + -0.0 528,785 + +0.3 528,785 + -0.8 528,785 + -0.7 528,786 - +0.5 528,786 - +0.5 528,786 - -0.3 528,786 - +0.2 528,786 - +0.3 528,786 - +1.4 528,786 - +1.0 528,786 - -0.4 528,786 - +0.4 528,786 - -0.1 528,786 - +0.2 528,786 - -0.1 528,786 - -0.2 528,834 + +0.0 528,834 + +0.0 528,835 - -0.1 528,860 + +0.4 528,861 - +0.2 528,861 - -0.7 528,904 + A4249_RS16480 0.15 +0.1 528,904 + A4249_RS16480 0.15 -2.1 528,904 + A4249_RS16480 0.15 +0.1 528,905 - A4249_RS16480 0.15 -0.1 528,923 - A4249_RS16480 0.17 -0.1 528,953 + A4249_RS16480 0.21 -0.4 528,953 + A4249_RS16480 0.21 -0.5 528,953 + A4249_RS16480 0.21 -0.4 528,953 + A4249_RS16480 0.21 -0.2 528,953 + A4249_RS16480 0.21 +0.5 528,953 + A4249_RS16480 0.21 +0.4 528,953 + A4249_RS16480 0.21 -0.2 528,953 + A4249_RS16480 0.21 -0.1 528,953 + A4249_RS16480 0.21 +0.2 528,954 - A4249_RS16480 0.21 +0.2 528,954 - A4249_RS16480 0.21 -0.3 528,954 - A4249_RS16480 0.21 +0.3 528,954 - A4249_RS16480 0.21 -0.5 528,954 - A4249_RS16480 0.21 +0.2 528,954 - A4249_RS16480 0.21 -0.5 528,954 - A4249_RS16480 0.21 +0.1 528,954 - A4249_RS16480 0.21 -0.3 528,954 - A4249_RS16480 0.21 -0.3 528,954 - A4249_RS16480 0.21 +0.7 528,964 + A4249_RS16480 0.22 +0.0 528,965 - A4249_RS16480 0.22 +0.7 528,965 - A4249_RS16480 0.22 +0.1 529,071 + A4249_RS16480 0.36 -0.7 529,071 + A4249_RS16480 0.36 -1.2 529,071 + A4249_RS16480 0.36 -0.5 529,071 + A4249_RS16480 0.36 +0.3 529,071 + A4249_RS16480 0.36 -0.1 529,072 - A4249_RS16480 0.36 +0.5 529,072 - A4249_RS16480 0.36 -0.6 529,072 - A4249_RS16480 0.36 +0.4 529,072 - A4249_RS16480 0.36 -0.5 529,072 - A4249_RS16480 0.36 +0.5 529,072 - A4249_RS16480 0.36 -0.7 529,072 - A4249_RS16480 0.36 +0.8 529,158 + A4249_RS16480 0.47 +0.4 529,158 + A4249_RS16480 0.47 -0.2 529,158 + A4249_RS16480 0.47 -0.9 529,158 + A4249_RS16480 0.47 -0.3 529,158 + A4249_RS16480 0.47 -0.7 529,159 - A4249_RS16480 0.47 -0.2 529,159 - A4249_RS16480 0.47 +0.8 529,159 - A4249_RS16480 0.47 -0.2 529,159 - A4249_RS16480 0.47 +1.3 529,159 - A4249_RS16480 0.47 -0.3 529,159 - A4249_RS16480 0.47 +0.5 529,177 - A4249_RS16480 0.49 +1.0 529,177 - A4249_RS16480 0.49 -0.1 529,177 - A4249_RS16480 0.49 -0.7 529,177 - A4249_RS16480 0.49 -0.8 529,192 + A4249_RS16480 0.51 +0.3 529,235 + A4249_RS16480 0.56 -0.2 529,235 + A4249_RS16480 0.56 +0.7 529,235 + A4249_RS16480 0.56 -0.0 529,236 - A4249_RS16480 0.57 -0.1 529,236 - A4249_RS16480 0.57 +0.9 529,271 + A4249_RS16480 0.61 -0.5 529,271 + A4249_RS16480 0.61 +0.2 529,271 + A4249_RS16480 0.61 -0.5 529,272 - A4249_RS16480 0.61 +1.3 529,272 - A4249_RS16480 0.61 -0.2 529,272 - A4249_RS16480 0.61 -0.7 529,273 + A4249_RS16480 0.61 +0.6 529,274 - A4249_RS16480 0.61 +1.2 529,274 - A4249_RS16480 0.61 -0.0 529,282 + A4249_RS16480 0.62 +0.6 529,282 + A4249_RS16480 0.62 +0.2 529,282 + A4249_RS16480 0.62 -0.2 529,283 - A4249_RS16480 0.62 -0.1 529,285 + A4249_RS16480 0.63 +0.0 529,383 + A4249_RS16480 0.75 -0.8 529,421 + A4249_RS16480 0.80 +0.2 529,421 + A4249_RS16480 0.80 -0.0 529,421 + A4249_RS16480 0.80 +0.1 529,422 - A4249_RS16480 0.80 +0.5 529,436 - A4249_RS16480 0.82 +0.6 529,513 + +0.5 529,521 + -0.2 529,522 - +0.3 529,522 - +0.8 529,577 + +0.3 529,578 - -0.2 529,578 - -0.2 529,578 - +0.5 529,591 + -0.1 529,591 + -0.0 529,592 - -0.2 529,592 - +0.0 529,595 + +0.0 529,595 + +0.6 529,595 + +1.2 529,595 + +1.3 529,596 - -1.3 529,596 - -2.3 529,708 + -0.4 529,792 + A4249_RS02465 0.10 -0.4 529,792 + A4249_RS02465 0.10 -0.1 529,792 + A4249_RS02465 0.10 -0.1 529,792 + A4249_RS02465 0.10 -0.4 529,792 + A4249_RS02465 0.10 +0.1 529,793 - A4249_RS02465 0.10 -0.0 529,793 - A4249_RS02465 0.10 -0.6 529,793 - A4249_RS02465 0.10 +0.1 529,793 - A4249_RS02465 0.10 -1.8 530,016 + A4249_RS02465 0.23 -1.1 530,016 + A4249_RS02465 0.23 +0.2 530,416 + A4249_RS02465 0.46 -0.8 530,416 + A4249_RS02465 0.46 +0.3 530,416 + A4249_RS02465 0.46 +0.8 530,416 + A4249_RS02465 0.46 +0.0 530,416 + A4249_RS02465 0.46 +0.8 530,417 - A4249_RS02465 0.46 -0.5 530,417 - A4249_RS02465 0.46 -1.4 530,417 - A4249_RS02465 0.46 -0.1 530,417 - A4249_RS02465 0.46 +0.0 530,417 - A4249_RS02465 0.46 -0.2 530,417 - A4249_RS02465 0.46 -0.0 530,417 - A4249_RS02465 0.46 -0.0 530,417 - A4249_RS02465 0.46 -0.6 530,632 + A4249_RS02465 0.58 -0.2 530,632 + A4249_RS02465 0.58 +0.0 530,633 - A4249_RS02465 0.58 -0.1 530,633 - A4249_RS02465 0.58 -0.1 530,633 - A4249_RS02465 0.58 -0.2 530,633 - A4249_RS02465 0.58 -0.7 530,687 - A4249_RS02465 0.62 +0.4 530,687 - A4249_RS02465 0.62 +0.0 530,719 + A4249_RS02465 0.63 +0.8 530,719 + A4249_RS02465 0.63 +0.5 530,719 + A4249_RS02465 0.63 -0.6 530,719 + A4249_RS02465 0.63 +0.2 530,719 + A4249_RS02465 0.63 -0.8 530,720 - A4249_RS02465 0.63 -0.7 530,720 - A4249_RS02465 0.63 +0.7 530,720 - A4249_RS02465 0.63 -0.2 530,720 - A4249_RS02465 0.63 -1.0 530,720 - A4249_RS02465 0.63 -1.3 530,720 - A4249_RS02465 0.63 -0.4 530,720 - A4249_RS02465 0.63 -0.1 530,720 - A4249_RS02465 0.63 +0.3 530,773 + A4249_RS02465 0.66 -0.3 530,773 + A4249_RS02465 0.66 -1.4 530,773 + A4249_RS02465 0.66 -0.7 530,773 + A4249_RS02465 0.66 -0.1 530,773 + A4249_RS02465 0.66 +0.0 530,773 + A4249_RS02465 0.66 -0.6 530,773 + A4249_RS02465 0.66 -1.3 530,773 + A4249_RS02465 0.66 -0.2 530,774 - A4249_RS02465 0.66 -1.0 530,774 - A4249_RS02465 0.66 +0.4 530,774 - A4249_RS02465 0.66 -0.5 530,947 + A4249_RS02465 0.76 -0.0 530,947 + A4249_RS02465 0.76 +0.6 530,947 + A4249_RS02465 0.76 -0.0 530,947 + A4249_RS02465 0.76 -0.0 530,947 + A4249_RS02465 0.76 +1.3 530,947 + A4249_RS02465 0.76 +0.1 530,947 + A4249_RS02465 0.76 -0.6 530,947 + A4249_RS02465 0.76 +0.3 530,947 + A4249_RS02465 0.76 -1.4 530,947 + A4249_RS02465 0.76 -0.5 530,947 + A4249_RS02465 0.76 -0.2 530,947 + A4249_RS02465 0.76 -0.9 530,947 + A4249_RS02465 0.76 -1.5 530,947 + A4249_RS02465 0.76 -0.5 530,947 + A4249_RS02465 0.76 -1.0 530,947 + A4249_RS02465 0.76 +0.0 530,947 + A4249_RS02465 0.76 -1.7 530,948 - A4249_RS02465 0.76 +0.0 530,948 - A4249_RS02465 0.76 -0.4 530,948 - A4249_RS02465 0.76 +0.4 530,948 - A4249_RS02465 0.76 -0.6 530,948 - A4249_RS02465 0.76 -0.3 530,948 - A4249_RS02465 0.76 +0.2 530,948 - A4249_RS02465 0.76 +0.2 530,949 + A4249_RS02465 0.77 -0.5 530,949 + A4249_RS02465 0.77 +1.6 530,949 + A4249_RS02465 0.77 +0.3 530,949 + A4249_RS02465 0.77 +0.0 530,949 + A4249_RS02465 0.77 +1.3 530,949 + A4249_RS02465 0.77 -0.5 530,949 + A4249_RS02465 0.77 +0.0 530,950 - A4249_RS02465 0.77 +0.6 530,950 - A4249_RS02465 0.77 +1.2 531,130 + A4249_RS02465 0.87 +0.9 531,130 + A4249_RS02465 0.87 -1.3 531,131 - A4249_RS02465 0.87 +1.0 531,346 + +0.4 531,346 + -1.0 531,347 - -0.4 531,347 - +0.1 531,347 - +0.1 531,347 - +0.0 531,386 - +0.7 531,386 - -0.2 531,386 - -0.2 531,386 - +0.1 531,390 - -0.3 531,390 - +0.5
Or see this region's nucleotide sequence