Strain Fitness in Escherichia coli ECRC98 around JDDGAC_28465

Experiment: WM_Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntJDDGAC_28455 and fcl are separated by 2 nucleotidesfcl and gmm overlap by 1 nucleotidesgmm and wcaI overlap by 4 nucleotides JDDGAC_28455: JDDGAC_28455 - GDP-mannose 4,6-dehydratase, at 5,455,550 to 5,456,671 _28455 JDDGAC_28460: fcl - GDP-L-fucose synthase, at 5,456,674 to 5,457,639 fcl JDDGAC_28465: gmm - GDP-mannose mannosyl hydrolase, at 5,457,639 to 5,458,121 gmm JDDGAC_28470: wcaI - colanic acid biosynthesis fucosyltransferase WcaI, at 5,458,118 to 5,459,341 wcaI Position (kb) 5457 5458 5459Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5456.669 kb on + strandat 5456.670 kb on - strandat 5456.673 kb on + strandat 5456.674 kb on - strandat 5456.769 kb on - strandat 5456.852 kb on - strand, within fclat 5457.045 kb on - strand, within fclat 5457.045 kb on - strand, within fclat 5457.122 kb on - strand, within fclat 5457.403 kb on - strand, within fclat 5457.599 kb on - strandat 5457.599 kb on - strandat 5457.599 kb on - strandat 5457.650 kb on - strandat 5457.914 kb on + strand, within gmmat 5457.945 kb on + strand, within gmmat 5457.946 kb on - strand, within gmmat 5458.125 kb on + strandat 5458.133 kb on + strandat 5458.146 kb on - strandat 5458.146 kb on - strandat 5458.176 kb on - strandat 5458.245 kb on - strand, within wcaIat 5458.245 kb on - strand, within wcaIat 5458.247 kb on + strand, within wcaIat 5458.334 kb on + strand, within wcaIat 5458.335 kb on - strand, within wcaIat 5458.542 kb on + strand, within wcaIat 5458.621 kb on + strand, within wcaIat 5458.621 kb on + strand, within wcaIat 5458.633 kb on + strand, within wcaIat 5458.676 kb on - strand, within wcaIat 5459.001 kb on - strand, within wcaI

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas09
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5,456,669 + -0.4
5,456,670 - -0.1
5,456,673 + -1.5
5,456,674 - +0.2
5,456,769 - +0.5
5,456,852 - fcl JDDGAC_28460 0.18 -2.2
5,457,045 - fcl JDDGAC_28460 0.38 +1.0
5,457,045 - fcl JDDGAC_28460 0.38 +0.5
5,457,122 - fcl JDDGAC_28460 0.46 -0.5
5,457,403 - fcl JDDGAC_28460 0.75 +0.8
5,457,599 - +2.1
5,457,599 - +0.3
5,457,599 - +0.1
5,457,650 - +0.9
5,457,914 + gmm JDDGAC_28465 0.57 -1.8
5,457,945 + gmm JDDGAC_28465 0.63 +1.3
5,457,946 - gmm JDDGAC_28465 0.64 +0.6
5,458,125 + +0.5
5,458,133 + -1.4
5,458,146 - -1.6
5,458,146 - -3.3
5,458,176 - -1.4
5,458,245 - wcaI JDDGAC_28470 0.10 -0.3
5,458,245 - wcaI JDDGAC_28470 0.10 +0.8
5,458,247 + wcaI JDDGAC_28470 0.11 -0.1
5,458,334 + wcaI JDDGAC_28470 0.18 -0.8
5,458,335 - wcaI JDDGAC_28470 0.18 -0.2
5,458,542 + wcaI JDDGAC_28470 0.35 +0.0
5,458,621 + wcaI JDDGAC_28470 0.41 -0.3
5,458,621 + wcaI JDDGAC_28470 0.41 -2.3
5,458,633 + wcaI JDDGAC_28470 0.42 -0.5
5,458,676 - wcaI JDDGAC_28470 0.46 +1.3
5,459,001 - wcaI JDDGAC_28470 0.72 -2.3

Or see this region's nucleotide sequence