Strain Fitness in Escherichia coli ECRC98 around JDDGAC_22290

Experiment: WM_Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnfeF and Ile2_trna are separated by 53 nucleotidesIle2_trna and mug are separated by 124 nucleotidesmug and rpoD are separated by 78 nucleotidesrpoD and dnaG are separated by 194 nucleotides JDDGAC_22275: nfeF - NADPH-dependent ferric chelate reductase, at 4,261,949 to 4,262,713 nfeF JDDGAC_22280: Ile2_trna - tRNA-Ile2(cat), at 4,262,767 to 4,262,842 _trna JDDGAC_22285: mug - G/U mismatch-specific DNA glycosylase, at 4,262,967 to 4,263,473 mug JDDGAC_22290: rpoD - RNA polymerase sigma factor RpoD, at 4,263,552 to 4,265,393 rpoD JDDGAC_22295: dnaG - DNA primase, at 4,265,588 to 4,267,333 dnaG Position (kb) 4263 4264 4265 4266Strain fitness (log2 ratio) -3 -2 -1 0 1at 4262.556 kb on + strand, within nfeFat 4262.597 kb on + strand, within nfeFat 4262.598 kb on - strand, within nfeFat 4262.598 kb on - strand, within nfeFat 4262.667 kb on - strandat 4262.719 kb on - strandat 4262.720 kb on + strandat 4262.761 kb on + strandat 4262.761 kb on + strandat 4262.762 kb on - strandat 4262.762 kb on - strandat 4262.762 kb on - strandat 4262.764 kb on - strandat 4262.855 kb on + strandat 4262.858 kb on - strandat 4262.962 kb on + strandat 4262.962 kb on + strandat 4262.963 kb on - strandat 4262.966 kb on + strandat 4263.088 kb on + strand, within mugat 4263.088 kb on + strand, within mugat 4263.089 kb on - strand, within mugat 4263.090 kb on + strand, within mugat 4263.091 kb on - strand, within mugat 4263.236 kb on + strand, within mugat 4263.301 kb on - strand, within mugat 4263.301 kb on - strand, within mug

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas09
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4,262,556 + nfeF JDDGAC_22275 0.79 +0.0
4,262,597 + nfeF JDDGAC_22275 0.85 -2.4
4,262,598 - nfeF JDDGAC_22275 0.85 +1.3
4,262,598 - nfeF JDDGAC_22275 0.85 -0.1
4,262,667 - +0.5
4,262,719 - +0.4
4,262,720 + -2.0
4,262,761 + -2.7
4,262,761 + -0.3
4,262,762 - -1.1
4,262,762 - +0.4
4,262,762 - -2.1
4,262,764 - -1.6
4,262,855 + +0.8
4,262,858 - -1.3
4,262,962 + +0.9
4,262,962 + -0.5
4,262,963 - +1.5
4,262,966 + -0.1
4,263,088 + mug JDDGAC_22285 0.24 +0.3
4,263,088 + mug JDDGAC_22285 0.24 +0.7
4,263,089 - mug JDDGAC_22285 0.24 -1.9
4,263,090 + mug JDDGAC_22285 0.24 -3.7
4,263,091 - mug JDDGAC_22285 0.24 -0.4
4,263,236 + mug JDDGAC_22285 0.53 -0.9
4,263,301 - mug JDDGAC_22285 0.66 -1.2
4,263,301 - mug JDDGAC_22285 0.66 -0.0

Or see this region's nucleotide sequence