Experiment: WM_Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nusA and infB are separated by 24 nucleotides infB and rbfA are separated by 164 nucleotides rbfA and truB overlap by 1 nucleotides
JDDGAC_21730: nusA - Transcription termination/antitermination protein NusA, at 4,158,059 to 4,159,546
nusA
JDDGAC_21735: infB - Translation initiation factor IF-2, at 4,159,571 to 4,162,243
infB
JDDGAC_21745: rbfA - 30S ribosome-binding factor RbfA, at 4,162,408 to 4,162,809
rbfA
JDDGAC_21750: truB - tRNA pseudouridine(55) synthase TruB, at 4,162,809 to 4,163,753
truB
Position (kb) 4159
4160
4161
4162
4163 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4159.377 kb on + strand, within nusA at 4159.377 kb on + strand, within nusA at 4159.420 kb on + strand at 4159.516 kb on + strand at 4162.231 kb on + strand at 4162.231 kb on + strand at 4162.732 kb on - strand, within rbfA at 4162.732 kb on - strand, within rbfA at 4162.807 kb on + strand at 4162.874 kb on + strand at 4162.910 kb on + strand, within truB at 4162.911 kb on - strand, within truB at 4162.912 kb on + strand, within truB at 4162.912 kb on + strand, within truB at 4162.912 kb on + strand, within truB at 4162.913 kb on - strand, within truB at 4162.914 kb on + strand, within truB at 4162.914 kb on + strand, within truB at 4162.914 kb on + strand, within truB at 4162.915 kb on - strand, within truB at 4162.915 kb on - strand, within truB at 4162.915 kb on - strand, within truB at 4162.915 kb on - strand, within truB at 4162.915 kb on - strand, within truB at 4162.916 kb on + strand, within truB at 4162.916 kb on + strand, within truB at 4162.916 kb on + strand, within truB at 4162.917 kb on - strand, within truB at 4162.917 kb on - strand, within truB at 4163.064 kb on - strand, within truB at 4163.064 kb on - strand, within truB at 4163.069 kb on + strand, within truB at 4163.069 kb on + strand, within truB at 4163.148 kb on - strand, within truB at 4163.184 kb on + strand, within truB at 4163.199 kb on + strand, within truB at 4163.200 kb on - strand, within truB at 4163.223 kb on + strand, within truB at 4163.224 kb on - strand, within truB
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas09 remove 4,159,377 + nusA JDDGAC_21730 0.89 +0.3 4,159,377 + nusA JDDGAC_21730 0.89 +0.2 4,159,420 + -0.1 4,159,516 + +1.6 4,162,231 + +0.4 4,162,231 + -2.1 4,162,732 - rbfA JDDGAC_21745 0.81 -0.6 4,162,732 - rbfA JDDGAC_21745 0.81 +0.3 4,162,807 + +0.9 4,162,874 + +0.3 4,162,910 + truB JDDGAC_21750 0.11 +1.6 4,162,911 - truB JDDGAC_21750 0.11 -1.2 4,162,912 + truB JDDGAC_21750 0.11 +2.2 4,162,912 + truB JDDGAC_21750 0.11 -2.1 4,162,912 + truB JDDGAC_21750 0.11 -1.5 4,162,913 - truB JDDGAC_21750 0.11 -2.4 4,162,914 + truB JDDGAC_21750 0.11 +0.5 4,162,914 + truB JDDGAC_21750 0.11 +1.4 4,162,914 + truB JDDGAC_21750 0.11 -1.9 4,162,915 - truB JDDGAC_21750 0.11 -1.2 4,162,915 - truB JDDGAC_21750 0.11 +1.1 4,162,915 - truB JDDGAC_21750 0.11 -1.1 4,162,915 - truB JDDGAC_21750 0.11 +0.4 4,162,915 - truB JDDGAC_21750 0.11 -0.5 4,162,916 + truB JDDGAC_21750 0.11 +0.3 4,162,916 + truB JDDGAC_21750 0.11 +0.8 4,162,916 + truB JDDGAC_21750 0.11 -0.0 4,162,917 - truB JDDGAC_21750 0.11 +0.7 4,162,917 - truB JDDGAC_21750 0.11 -2.2 4,163,064 - truB JDDGAC_21750 0.27 +0.4 4,163,064 - truB JDDGAC_21750 0.27 +0.3 4,163,069 + truB JDDGAC_21750 0.28 +1.3 4,163,069 + truB JDDGAC_21750 0.28 -0.7 4,163,148 - truB JDDGAC_21750 0.36 -1.5 4,163,184 + truB JDDGAC_21750 0.40 -0.7 4,163,199 + truB JDDGAC_21750 0.41 -2.6 4,163,200 - truB JDDGAC_21750 0.41 -1.8 4,163,223 + truB JDDGAC_21750 0.44 +0.3 4,163,224 - truB JDDGAC_21750 0.44 +1.3
Or see this region's nucleotide sequence