Experiment: WM_Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nanE and nanK overlap by 4 nucleotides nanK and nanQ overlap by 4 nucleotides nanQ and yhcG are separated by 59 nucleotides
JDDGAC_21485: nanE - Putative N-acetylmannosamine-6-phosphate 2-epimerase, at 4,111,722 to 4,112,411
nanE
JDDGAC_21490: nanK - N-acetylmannosamine kinase, at 4,112,408 to 4,113,283
nanK
JDDGAC_21495: nanQ - N-acetylneuraminate anomerase, at 4,113,280 to 4,113,744
nanQ
JDDGAC_21500: yhcG - Putative nuclease YhcG, at 4,113,804 to 4,114,931
yhcG
Position (kb)
4113
4114 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4112.281 kb on - strand, within nanE at 4112.351 kb on + strand at 4112.407 kb on + strand at 4112.408 kb on - strand at 4112.440 kb on + strand at 4112.440 kb on + strand at 4112.441 kb on - strand at 4112.550 kb on + strand, within nanK at 4112.748 kb on - strand, within nanK at 4112.768 kb on - strand, within nanK at 4112.786 kb on - strand, within nanK at 4112.875 kb on + strand, within nanK at 4113.243 kb on + strand at 4113.243 kb on + strand at 4113.244 kb on - strand at 4113.280 kb on - strand at 4113.296 kb on + strand at 4113.356 kb on + strand, within nanQ at 4113.357 kb on - strand, within nanQ at 4113.436 kb on - strand, within nanQ at 4113.504 kb on - strand, within nanQ at 4113.504 kb on - strand, within nanQ at 4113.623 kb on + strand, within nanQ at 4113.624 kb on - strand, within nanQ at 4113.648 kb on + strand, within nanQ at 4113.723 kb on + strand at 4113.734 kb on + strand at 4113.749 kb on + strand at 4113.749 kb on + strand at 4113.749 kb on + strand at 4113.750 kb on - strand at 4113.816 kb on - strand at 4113.880 kb on - strand at 4113.890 kb on + strand at 4113.890 kb on + strand at 4113.891 kb on - strand at 4113.891 kb on - strand at 4113.891 kb on - strand at 4113.891 kb on - strand at 4113.892 kb on + strand at 4113.892 kb on + strand at 4113.893 kb on - strand at 4113.934 kb on + strand, within yhcG at 4113.935 kb on - strand, within yhcG at 4114.031 kb on - strand, within yhcG at 4114.034 kb on + strand, within yhcG at 4114.034 kb on + strand, within yhcG at 4114.034 kb on + strand, within yhcG at 4114.035 kb on - strand, within yhcG at 4114.035 kb on - strand, within yhcG at 4114.035 kb on - strand, within yhcG at 4114.035 kb on - strand, within yhcG at 4114.035 kb on - strand, within yhcG at 4114.035 kb on - strand, within yhcG at 4114.035 kb on - strand, within yhcG at 4114.055 kb on + strand, within yhcG at 4114.057 kb on + strand, within yhcG at 4114.089 kb on + strand, within yhcG at 4114.155 kb on + strand, within yhcG at 4114.319 kb on + strand, within yhcG at 4114.320 kb on - strand, within yhcG at 4114.320 kb on - strand, within yhcG at 4114.456 kb on + strand, within yhcG at 4114.501 kb on + strand, within yhcG at 4114.502 kb on - strand, within yhcG at 4114.603 kb on - strand, within yhcG at 4114.697 kb on + strand, within yhcG at 4114.698 kb on - strand, within yhcG at 4114.698 kb on - strand, within yhcG at 4114.699 kb on + strand, within yhcG at 4114.700 kb on - strand, within yhcG at 4114.700 kb on - strand, within yhcG at 4114.700 kb on - strand, within yhcG at 4114.705 kb on - strand, within yhcG at 4114.710 kb on + strand, within yhcG at 4114.710 kb on + strand, within yhcG at 4114.735 kb on - strand, within yhcG at 4114.735 kb on - strand, within yhcG
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas09 remove 4,112,281 - nanE JDDGAC_21485 0.81 +0.5 4,112,351 + +0.2 4,112,407 + +0.7 4,112,408 - -1.7 4,112,440 + +0.8 4,112,440 + -3.1 4,112,441 - +0.3 4,112,550 + nanK JDDGAC_21490 0.16 -0.9 4,112,748 - nanK JDDGAC_21490 0.39 +0.8 4,112,768 - nanK JDDGAC_21490 0.41 +0.4 4,112,786 - nanK JDDGAC_21490 0.43 -0.6 4,112,875 + nanK JDDGAC_21490 0.53 +0.4 4,113,243 + +2.0 4,113,243 + -0.5 4,113,244 - +0.7 4,113,280 - +1.6 4,113,296 + -0.5 4,113,356 + nanQ JDDGAC_21495 0.16 -0.5 4,113,357 - nanQ JDDGAC_21495 0.17 -0.8 4,113,436 - nanQ JDDGAC_21495 0.34 -1.4 4,113,504 - nanQ JDDGAC_21495 0.48 +0.9 4,113,504 - nanQ JDDGAC_21495 0.48 +0.4 4,113,623 + nanQ JDDGAC_21495 0.74 +0.6 4,113,624 - nanQ JDDGAC_21495 0.74 -1.7 4,113,648 + nanQ JDDGAC_21495 0.79 +1.4 4,113,723 + -0.8 4,113,734 + -0.1 4,113,749 + -1.4 4,113,749 + -0.2 4,113,749 + -3.0 4,113,750 - +1.8 4,113,816 - -2.2 4,113,880 - -0.3 4,113,890 + -2.0 4,113,890 + -1.1 4,113,891 - +0.8 4,113,891 - -2.6 4,113,891 - +0.4 4,113,891 - -1.1 4,113,892 + +1.7 4,113,892 + -1.0 4,113,893 - -0.9 4,113,934 + yhcG JDDGAC_21500 0.12 +0.0 4,113,935 - yhcG JDDGAC_21500 0.12 -0.2 4,114,031 - yhcG JDDGAC_21500 0.20 -0.6 4,114,034 + yhcG JDDGAC_21500 0.20 -0.6 4,114,034 + yhcG JDDGAC_21500 0.20 -0.9 4,114,034 + yhcG JDDGAC_21500 0.20 -2.7 4,114,035 - yhcG JDDGAC_21500 0.20 +0.4 4,114,035 - yhcG JDDGAC_21500 0.20 -0.1 4,114,035 - yhcG JDDGAC_21500 0.20 -0.2 4,114,035 - yhcG JDDGAC_21500 0.20 +1.7 4,114,035 - yhcG JDDGAC_21500 0.20 -1.4 4,114,035 - yhcG JDDGAC_21500 0.20 -1.2 4,114,035 - yhcG JDDGAC_21500 0.20 +0.3 4,114,055 + yhcG JDDGAC_21500 0.22 +0.2 4,114,057 + yhcG JDDGAC_21500 0.22 +0.2 4,114,089 + yhcG JDDGAC_21500 0.25 +1.1 4,114,155 + yhcG JDDGAC_21500 0.31 -0.2 4,114,319 + yhcG JDDGAC_21500 0.46 -0.1 4,114,320 - yhcG JDDGAC_21500 0.46 +0.1 4,114,320 - yhcG JDDGAC_21500 0.46 +0.2 4,114,456 + yhcG JDDGAC_21500 0.58 -0.6 4,114,501 + yhcG JDDGAC_21500 0.62 -0.6 4,114,502 - yhcG JDDGAC_21500 0.62 +1.7 4,114,603 - yhcG JDDGAC_21500 0.71 +1.5 4,114,697 + yhcG JDDGAC_21500 0.79 -0.1 4,114,698 - yhcG JDDGAC_21500 0.79 -0.3 4,114,698 - yhcG JDDGAC_21500 0.79 -0.0 4,114,699 + yhcG JDDGAC_21500 0.79 -2.5 4,114,700 - yhcG JDDGAC_21500 0.79 -0.1 4,114,700 - yhcG JDDGAC_21500 0.79 +2.1 4,114,700 - yhcG JDDGAC_21500 0.79 -0.6 4,114,705 - yhcG JDDGAC_21500 0.80 -2.3 4,114,710 + yhcG JDDGAC_21500 0.80 +0.3 4,114,710 + yhcG JDDGAC_21500 0.80 +1.8 4,114,735 - yhcG JDDGAC_21500 0.83 -1.3 4,114,735 - yhcG JDDGAC_21500 0.83 +0.5
Or see this region's nucleotide sequence