Strain Fitness in Escherichia coli ECRC98 around JDDGAC_10250

Experiment: WM_Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntJDDGAC_10245 and JDDGAC_10250 are separated by 211 nucleotidesJDDGAC_10250 and ninG overlap by 4 nucleotides JDDGAC_10245: JDDGAC_10245 - DUF2219 domain-containing protein, at 1,826,465 to 1,827,424 _10245 JDDGAC_10250: JDDGAC_10250 - Serine/threonine-protein phosphatase, at 1,827,636 to 1,828,301 _10250 JDDGAC_10255: ninG - Protein ninG, at 1,828,298 to 1,828,921 ninG Position (kb) 1827 1828 1829Strain fitness (log2 ratio) -2 -1 0 1 2at 1826.705 kb on - strand, within JDDGAC_10245at 1826.774 kb on + strand, within JDDGAC_10245at 1826.844 kb on + strand, within JDDGAC_10245at 1826.844 kb on + strand, within JDDGAC_10245at 1826.844 kb on + strand, within JDDGAC_10245at 1826.845 kb on - strand, within JDDGAC_10245at 1827.023 kb on + strand, within JDDGAC_10245at 1827.071 kb on + strand, within JDDGAC_10245at 1827.158 kb on - strand, within JDDGAC_10245at 1828.811 kb on + strand, within ninGat 1828.811 kb on + strand, within ninGat 1828.812 kb on - strand, within ninGat 1828.812 kb on - strand, within ninGat 1828.891 kb on + strandat 1828.892 kb on - strandat 1829.031 kb on + strandat 1829.060 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas09
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1,826,705 - JDDGAC_10245 0.25 -0.6
1,826,774 + JDDGAC_10245 0.32 -0.3
1,826,844 + JDDGAC_10245 0.39 +0.5
1,826,844 + JDDGAC_10245 0.39 -0.1
1,826,844 + JDDGAC_10245 0.39 -1.0
1,826,845 - JDDGAC_10245 0.40 +2.0
1,827,023 + JDDGAC_10245 0.58 -0.2
1,827,071 + JDDGAC_10245 0.63 +1.9
1,827,158 - JDDGAC_10245 0.72 -0.0
1,828,811 + ninG JDDGAC_10255 0.82 +1.1
1,828,811 + ninG JDDGAC_10255 0.82 -1.4
1,828,812 - ninG JDDGAC_10255 0.82 +2.0
1,828,812 - ninG JDDGAC_10255 0.82 +0.1
1,828,891 + -1.1
1,828,892 - -0.1
1,829,031 + +0.3
1,829,060 - -2.0

Or see this region's nucleotide sequence