Strain Fitness in Escherichia coli ECRC98 around JDDGAC_00440

Experiment: WM_Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinsI1 and gpD overlap by 50 nucleotidesgpD and JDDGAC_00450 are separated by 157 nucleotides JDDGAC_00440: insI1 - IS30 family transposase, at 76,228 to 77,262 insI1 JDDGAC_00445: gpD - phage tail protein, at 77,213 to 77,536 gpD JDDGAC_00450: JDDGAC_00450 - phage tail protein, at 77,694 to 78,878 _00450 Position (kb) 76 77 78Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 75.497 kb on - strandat 75.550 kb on - strandat 75.560 kb on + strandat 75.602 kb on - strandat 75.618 kb on - strandat 75.624 kb on - strandat 75.671 kb on + strandat 75.832 kb on + strandat 75.855 kb on + strandat 75.905 kb on + strandat 76.001 kb on - strandat 76.848 kb on + strand, within insI1at 76.850 kb on + strand, within insI1at 76.862 kb on + strand, within insI1at 76.881 kb on + strand, within insI1at 76.881 kb on + strand, within insI1at 77.148 kb on + strand, within insI1at 77.148 kb on + strand, within insI1at 77.162 kb on + strandat 77.232 kb on - strandat 77.266 kb on + strand, within gpDat 77.266 kb on + strand, within gpDat 77.455 kb on + strand, within gpDat 77.677 kb on + strandat 77.747 kb on - strandat 77.784 kb on - strandat 77.906 kb on + strand, within JDDGAC_00450at 78.027 kb on + strand, within JDDGAC_00450at 78.040 kb on - strand, within JDDGAC_00450at 78.172 kb on - strand, within JDDGAC_00450at 78.253 kb on + strand, within JDDGAC_00450

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas09
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75,497 - -0.8
75,550 - -0.1
75,560 + -1.7
75,602 - +1.0
75,618 - -0.1
75,624 - -0.5
75,671 + -2.0
75,832 + -1.4
75,855 + -0.2
75,905 + +0.8
76,001 - -2.5
76,848 + insI1 JDDGAC_00440 0.60 -0.4
76,850 + insI1 JDDGAC_00440 0.60 -0.6
76,862 + insI1 JDDGAC_00440 0.61 +0.1
76,881 + insI1 JDDGAC_00440 0.63 +1.2
76,881 + insI1 JDDGAC_00440 0.63 -2.2
77,148 + insI1 JDDGAC_00440 0.89 +1.6
77,148 + insI1 JDDGAC_00440 0.89 +1.2
77,162 + -0.2
77,232 - +0.7
77,266 + gpD JDDGAC_00445 0.16 -1.4
77,266 + gpD JDDGAC_00445 0.16 +1.6
77,455 + gpD JDDGAC_00445 0.75 +1.5
77,677 + -0.2
77,747 - -0.3
77,784 - -2.8
77,906 + JDDGAC_00450 0.18 -0.7
78,027 + JDDGAC_00450 0.28 -1.3
78,040 - JDDGAC_00450 0.29 -4.0
78,172 - JDDGAC_00450 0.40 +0.5
78,253 + JDDGAC_00450 0.47 +0.3

Or see this region's nucleotide sequence