Experiment: R2A with Apigenin 50 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR88_RS17710 and ABZR88_RS17715 are separated by 233 nucleotides ABZR88_RS17715 and ABZR88_RS17720 are separated by 99 nucleotides ABZR88_RS17720 and ABZR88_RS17725 are separated by 145 nucleotides
ABZR88_RS17710: ABZR88_RS17710 - polysaccharide biosynthesis/export family protein, at 4,329,957 to 4,330,736
_RS17710
ABZR88_RS17715: ABZR88_RS17715 - acyltransferase, at 4,330,970 to 4,332,226
_RS17715
ABZR88_RS17720: ABZR88_RS17720 - hypothetical protein, at 4,332,326 to 4,332,775
_RS17720
ABZR88_RS17725: ABZR88_RS17725 - DNA-directed RNA polymerase subunit beta', at 4,332,921 to 4,337,213
_RS17725
Position (kb) 4330
4331
4332
4333 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4330.013 kb on + strand at 4330.014 kb on - strand at 4330.140 kb on + strand, within ABZR88_RS17710 at 4330.333 kb on - strand, within ABZR88_RS17710 at 4330.440 kb on - strand, within ABZR88_RS17710 at 4330.662 kb on - strand at 4330.664 kb on + strand at 4330.665 kb on - strand at 4330.816 kb on - strand at 4330.930 kb on + strand at 4330.971 kb on + strand at 4331.073 kb on + strand at 4331.078 kb on - strand at 4331.124 kb on - strand, within ABZR88_RS17715 at 4331.135 kb on + strand, within ABZR88_RS17715 at 4331.142 kb on + strand, within ABZR88_RS17715 at 4331.143 kb on - strand, within ABZR88_RS17715 at 4331.198 kb on + strand, within ABZR88_RS17715 at 4331.272 kb on - strand, within ABZR88_RS17715 at 4331.290 kb on + strand, within ABZR88_RS17715 at 4331.291 kb on - strand, within ABZR88_RS17715 at 4331.472 kb on + strand, within ABZR88_RS17715 at 4331.473 kb on - strand, within ABZR88_RS17715 at 4331.528 kb on - strand, within ABZR88_RS17715 at 4331.594 kb on - strand, within ABZR88_RS17715 at 4331.670 kb on - strand, within ABZR88_RS17715 at 4331.760 kb on - strand, within ABZR88_RS17715 at 4331.849 kb on + strand, within ABZR88_RS17715 at 4331.850 kb on - strand, within ABZR88_RS17715 at 4331.850 kb on - strand, within ABZR88_RS17715 at 4331.872 kb on + strand, within ABZR88_RS17715 at 4332.048 kb on - strand, within ABZR88_RS17715 at 4332.144 kb on - strand at 4332.284 kb on + strand at 4332.354 kb on + strand at 4332.600 kb on - strand, within ABZR88_RS17720 at 4332.620 kb on - strand, within ABZR88_RS17720 at 4332.713 kb on + strand, within ABZR88_RS17720 at 4332.716 kb on - strand, within ABZR88_RS17720 at 4332.727 kb on + strand, within ABZR88_RS17720 at 4332.802 kb on + strand at 4332.803 kb on - strand at 4332.823 kb on - strand at 4332.825 kb on + strand at 4332.837 kb on - strand at 4332.852 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Apigenin 50 ug/ml remove 4,330,013 + -1.8 4,330,014 - -1.7 4,330,140 + ABZR88_RS17710 0.23 -3.2 4,330,333 - ABZR88_RS17710 0.48 -0.4 4,330,440 - ABZR88_RS17710 0.62 -2.3 4,330,662 - -2.9 4,330,664 + -1.9 4,330,665 - -2.4 4,330,816 - +0.8 4,330,930 + +3.2 4,330,971 + +1.6 4,331,073 + -0.3 4,331,078 - -0.7 4,331,124 - ABZR88_RS17715 0.12 -0.6 4,331,135 + ABZR88_RS17715 0.13 +0.4 4,331,142 + ABZR88_RS17715 0.14 -0.3 4,331,143 - ABZR88_RS17715 0.14 -0.5 4,331,198 + ABZR88_RS17715 0.18 +0.2 4,331,272 - ABZR88_RS17715 0.24 +0.0 4,331,290 + ABZR88_RS17715 0.25 +0.2 4,331,291 - ABZR88_RS17715 0.26 -0.3 4,331,472 + ABZR88_RS17715 0.40 +0.0 4,331,473 - ABZR88_RS17715 0.40 +0.5 4,331,528 - ABZR88_RS17715 0.44 +1.0 4,331,594 - ABZR88_RS17715 0.50 +0.1 4,331,670 - ABZR88_RS17715 0.56 +0.0 4,331,760 - ABZR88_RS17715 0.63 +0.4 4,331,849 + ABZR88_RS17715 0.70 -0.1 4,331,850 - ABZR88_RS17715 0.70 -0.3 4,331,850 - ABZR88_RS17715 0.70 -0.3 4,331,872 + ABZR88_RS17715 0.72 +0.5 4,332,048 - ABZR88_RS17715 0.86 +0.2 4,332,144 - -0.3 4,332,284 + -0.2 4,332,354 + -0.2 4,332,600 - ABZR88_RS17720 0.61 +1.0 4,332,620 - ABZR88_RS17720 0.65 +1.2 4,332,713 + ABZR88_RS17720 0.86 -0.3 4,332,716 - ABZR88_RS17720 0.87 -0.1 4,332,727 + ABZR88_RS17720 0.89 +0.2 4,332,802 + -0.1 4,332,803 - -0.1 4,332,823 - -0.4 4,332,825 + -0.2 4,332,837 - -0.0 4,332,852 - +1.4
Or see this region's nucleotide sequence